Next Generation Sequencing

DNA for SOLiD™

In general, library preparation of fragmented DNA and ChIP-Seq DNA for use with the SOLiD™ 4 sequencing platform involves repair of 3’ and 5’ ends before ligation to an adaptor. The final step in both workflows is amplification via polymerase chain reaction (PCR). For more information, choose the workflow tab below.

Libraries prepared in this manner are suitable for templated bead preparation, the next step in the SOLiD sequencing workflow.

NEBNext® reagents are available for each step in DNA and ChIP-Seq DNA library preparation workflows for the SOLiD platform. Reagents from NEB are available in a master mix format. Each of these reagents has been functionally validated by preparation of a genomic DNA and ChIP-Seq DNA followed by SOLiD sequencing.

SOLiD™ is a trademark owned by Life Technologies, Inc.
NEBNext® is a registered trademark of New England Biolabs, Inc.

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FAQs for DNA for SOLiD™

Protocols for DNA for SOLiD™

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

DNA Library Construction Workflow for SOLiD