Library preparation of fragmented DNA, ChIP DNA, and cDNA prepared from mRNA, for use with the SOLiD™ 4 sequencing platform, involves repair of 3’ and 5’ ends before ligation with an adaptor. Small RNA libraries are constructed by a different workflow, in which adaptors are ligated directly to the small RNA molecules, followed by reverse transcription and PCR amplification. Libraries prepared from DNA and mRNA are size selected after adaptor ligation and amplified via polymerase chain reaction (PCR). For more information, choose the workflow tabs below.
Libraries prepared in this manner are suitable for templated bead preparation, the next step in the SOLiD sequencing workflow.
NEBNext® reagents are available for each step in the library preparation workflow for the SOLiD platform. For DNA, ChIP, mRNA and small RNA library preparation, reagents from NEB are available in a master mix format. Each of these reagents has been functionally validated by preparation of a genomic DNA, ChIP-Seq DNA, mRNA or small RNA library followed by SOLiD sequencing.
SOLiD™ is a trademark owned by Life Technologies, Inc.
NEBNext® is a registered trademark of New England Biolabs, Inc.