Next Generation Sequencing
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  • SOLiD™ Library Preparation

    Library preparation of fragmented DNA, ChIP DNA, and cDNA prepared from mRNA, for use with the SOLiD™ 4 sequencing platform, involves repair of 3’ and 5’ ends before ligation with an adaptor. Small RNA libraries are constructed by a different workflow, in which adaptors are ligated directly to the small RNA molecules, followed by reverse transcription and PCR amplification. Libraries prepared from DNA and mRNA are size selected after adaptor ligation and amplified via polymerase chain reaction (PCR). For more information, choose the workflow tabs below.

    Libraries prepared in this manner are suitable for templated bead preparation, the next step in the SOLiD sequencing workflow.

    NEBNext® reagents are available for each step in the library preparation workflow for the SOLiD platform. For DNA, ChIP, mRNA and small RNA library preparation, reagents from NEB are available in a master mix format. Each of these reagents has been functionally validated by preparation of a genomic DNA, ChIP-Seq DNA, mRNA or small RNA library followed by SOLiD sequencing.

    SOLiD™ is a trademark owned by Life Technologies, Inc.
    NEBNext® is a registered trademark of New England Biolabs, Inc.
    1. Tips for Optimizing RNA Inputs in NGS Library Construction

      Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.

    2. Tips for Optimizing DNA Inputs in NGS Library Construction

      Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

    3. 12 Quick Tips for NGS Library Preparation

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    4. Why Choose NEBNext Reagents for NGS Library Prep?

      NEBNext products are a great choice for your NGS library preparation needs. In this video, Fiona shares a few reasons why.

    5. Tips for Bead-based Clean ups and Size Selection

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    6. NEBNext for Core Lab Facilities

      Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs.

    7. A Breakthrough Method of RNA Sample Preparation

      The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

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    SOLiD™ Library Preparation includes these areas of focus:

    DNA for SOLiD™
    RNA for SOLiD™

    FAQs for SOLiD™ Library Preparation

    Protocols for SOLiD™ Library Preparation

    DNA Library Construction Workflow for SOLiD

    mRNA Library Construction Workflow for SOLiD

    Small RNA Library Construction Workflow for SOLiD