Next Generation Sequencing
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  • NEBNext® Sample Preparation for Next Generation Sequencing

    NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for next generation sequencing, and target enrichment of DNA. Available for a wide range of sample types, NEBNext products are provided in user-friendly formats including kits and modules, with bulk or customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

    Use the following tool to determine the best NEBNext products for you:




    NEBNext® Sample Preparation for Next Generation Sequencing

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    OrderNEBNext Direct Cancer HotSpot Panel

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    OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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    OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ II Ligation Module 

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    OrderNEBNext® Ultra™ II Q5® Master Mix

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    OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

    Illumina® Library Preparation

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    OrderNEBNext Direct Cancer HotSpot Panel

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    OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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    OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

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    OrderNEBNext® dA-Tailing Module

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    OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® DNA Library Prep Reagent Set for Illumina®

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    OrderNEBNext® End Repair Module

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    OrderNEBNext® FFPE DNA Repair Mix

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    OrderNEBNext® mRNA Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® mRNA Library Prep Reagent Set for Illumina®

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    OrderNEBNext® mRNA Second Strand Synthesis Module

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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    OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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    OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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    OrderNEBNext® Quick Ligation Module

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    OrderNEBNext® RNA First Strand Synthesis Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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    OrderNEBNext® Singleplex Oligos for Illumina®

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    OrderNEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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    OrderNEBNext® Ultra™ Directional RNA Second Strand Synthesis Module

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    OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

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    OrderNEBNext® Ultra™ Ligation Module

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    OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ II Ligation Module 

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    OrderNEBNext® Ultra™ II Q5® Master Mix

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    OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

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    OrderNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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    OrderNEBNext® Library Dilution Buffer

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    OrderNEBNext® Library Quant Kit for Illumina®

    Ion Torrent™ DNA Library Preparation

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    OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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    OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

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    OrderNEBNext® FFPE DNA Repair Mix

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

    454™ Library Preparation

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    OrderNEBNext® DNA Library Prep Master Mix Set for 454™

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    OrderNEBNext® End Repair Module

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    OrderNEBNext® mRNA Second Strand Synthesis Module

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® Quick Ligation Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

    SOLiD™ Library Preparation

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1)

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

    DNA Library Preparation

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    OrderNEBNext® High-Fidelity 2X PCR Master Mix

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    OrderNEBNext Direct Cancer HotSpot Panel

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    OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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    OrderNEBNext® dA-Tailing Reaction Buffer

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    OrderNEBNext® End Repair Reaction Buffer

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    OrderNEBNext® FFPE DNA Repair Mix

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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    OrderNEBNext® Quick Ligation Reaction Buffer

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    OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ II Ligation Module 

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    OrderNEBNext® Ultra™ II Q5® Master Mix

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    OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

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    OrderNEBNext® Library Dilution Buffer

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    OrderNEBNext® Library Quant Kit for Illumina®

    RNA Library Preparation

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    Order5´ DNA Adenylation Kit

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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    OrderNEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

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    OrderNEBNext® Library Dilution Buffer

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    OrderNEBNext® Library Quant Kit for Illumina®

    DNA Fragmentation

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    OrderNEBNext® dsDNA Fragmentase® Reaction Buffer

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    OrderNEBNext® dsDNA Fragmentase®

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    OrderNEBNext® dsDNA Fragmentase® Reaction Buffer v2

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    OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

    FFPE DNA Repair

    NEBNext® Automation

    Illumina® Library Preparation

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    OrderNEBNext Direct Cancer HotSpot Panel

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    OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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    OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

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    OrderNEBNext® dA-Tailing Module

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    OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® DNA Library Prep Reagent Set for Illumina®

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    OrderNEBNext® End Repair Module

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    OrderNEBNext® FFPE DNA Repair Mix

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    OrderNEBNext® mRNA Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® mRNA Library Prep Reagent Set for Illumina®

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    OrderNEBNext® mRNA Second Strand Synthesis Module

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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    OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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    OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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    OrderNEBNext® Quick Ligation Module

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    OrderNEBNext® RNA First Strand Synthesis Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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    OrderNEBNext® Singleplex Oligos for Illumina®

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    OrderNEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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    OrderNEBNext® Ultra™ Directional RNA Second Strand Synthesis Module

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    OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

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    OrderNEBNext® Ultra™ Ligation Module

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    OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ II Ligation Module 

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    OrderNEBNext® Ultra™ II Q5® Master Mix

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    OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

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    OrderNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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    OrderNEBNext® Library Dilution Buffer

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    OrderNEBNext® Library Quant Kit for Illumina®

    NEBNext® Ultra™ II DNA Library Prep

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    OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ II Ligation Module 

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    OrderNEBNext® Ultra™ II Q5® Master Mix

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    OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

    DNA for Illumina®

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    OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

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    OrderNEBNext® dA-Tailing Module

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    OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® DNA Library Prep Reagent Set for Illumina®

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    OrderNEBNext® End Repair Module

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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    OrderNEBNext® Quick Ligation Module

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    OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

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    OrderNEBNext® Ultra™ Ligation Module

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    OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

    RNA for Illumina®

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    OrderNEBNext® mRNA Library Prep Master Mix Set for Illumina®

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    OrderNEBNext® mRNA Library Prep Reagent Set for Illumina®

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    OrderNEBNext® mRNA Second Strand Synthesis Module

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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    OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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    OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® RNA First Strand Synthesis Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

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    OrderNEBNext® Singleplex Oligos for Illumina®

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    OrderNEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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    OrderNEBNext® Ultra™ Directional RNA Second Strand Synthesis Module

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    OrderNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ RNA Library Prep Kit for Illumina®

    Ion Torrent™ DNA Library Preparation

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    OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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    OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

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    OrderNEBNext® FFPE DNA Repair Mix

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

    DNA for Ion Torrent™

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    OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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    OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

    454™ Library Preparation

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    OrderNEBNext® DNA Library Prep Master Mix Set for 454™

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    OrderNEBNext® End Repair Module

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    OrderNEBNext® mRNA Second Strand Synthesis Module

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® Quick Ligation Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

    DNA for 454™

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    OrderNEBNext® DNA Library Prep Master Mix Set for 454™

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    OrderNEBNext® End Repair Module

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    OrderNEBNext® Quick Ligation Module

    RNA for 454™

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    OrderNEBNext® mRNA Second Strand Synthesis Module

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

    SOLiD™ Library Preparation

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1)

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

    DNA for SOLiD™

    RNA for SOLiD™

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    OrderNEBNext® Magnesium RNA Fragmentation Module

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    NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1)

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    OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

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    OrderNEBNext® RNase III RNA Fragmentation Module

    DNA Library Preparation

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    OrderNEBNext® High-Fidelity 2X PCR Master Mix

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    OrderNEBNext Direct Cancer HotSpot Panel

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    OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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    OrderNEBNext® dA-Tailing Reaction Buffer

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    OrderNEBNext® End Repair Reaction Buffer

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    OrderNEBNext® FFPE DNA Repair Mix

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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    OrderNEBNext® Quick Ligation Reaction Buffer

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    OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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    OrderNEBNext® Ultra™ II Ligation Module 

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    OrderNEBNext® Ultra™ II Q5® Master Mix

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    OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

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    OrderNEBNext® Library Dilution Buffer

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    OrderNEBNext® Library Quant Kit for Illumina®

    RNA Library Preparation

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    Order5´ DNA Adenylation Kit

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    OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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    OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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    OrderNEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

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    OrderNEBNext® Library Dilution Buffer

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    OrderNEBNext® Library Quant Kit for Illumina®

    DNA Fragmentation

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    OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

    NEBNext® dsDNA Fragmentase®

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    OrderNEBNext® dsDNA Fragmentase® Reaction Buffer

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    OrderNEBNext® dsDNA Fragmentase®

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    OrderNEBNext® dsDNA Fragmentase® Reaction Buffer v2

    FFPE DNA Repair

    NEBNext FFPE DNA Repair Mix

    NEBNext® Automation

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