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NEBNext® Sample Preparation for Next Generation Sequencing

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for next generation sequencing, and target enrichment of DNA. Available for a wide range of sample types, NEBNext products are provided in user-friendly formats including kits and modules, with bulk or customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

Use the following tool to determine the best NEBNext products for you:

NEBNext® Sample Preparation for Next Generation Sequencing

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Order NEBNext Direct® BRCA1/BRCA2 Panel

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Order NEBNext Direct® Cancer HotSpot Panel

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Order NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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Order NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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Order NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra™ II Ligation Module 

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Order NEBNext® Ultra™ II Q5® Master Mix

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Order NEBNext® Ultra™ II End Repair/dA-Tailing Module

Illumina® Library Preparation

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Order NEBNext Direct® BRCA1/BRCA2 Panel

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Order NEBNext Direct® Cancer HotSpot Panel

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Order NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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Order NEBNext® dA-Tailing Module

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Order NEBNext® End Repair Module

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Order NEBNext® FFPE DNA Repair Mix

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Order NEBNext® Library Dilution Buffer

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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Order NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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Order NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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Order NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order NEBNext® Quick Ligation Module

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Order NEBNext® RNA First Strand Synthesis Module

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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Order NEBNext® Singleplex Oligos for Illumina®

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Order NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra™ End Repair/dA-Tailing Module

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Order NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Module

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Order NEBNext® Ultra™ II Ligation Module 

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Order NEBNext® Ultra™ II Q5® Master Mix

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Order NEBNext® Ultra™ II End Repair/dA-Tailing Module

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Order NEBNext® Ultra™ Ligation Module

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Order NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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Order NEBNext® DNA Library Prep Master Mix Set for Illumina®

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Order NEBNext® Library Quant Kit for Illumina®

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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Order NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® RNA First Strand Synthesis Module

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Order NEBNext® Singleplex Oligos for Illumina®

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Order NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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Order NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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Order NEBNext® dA-Tailing Module

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Order NEBNext® End Repair Module

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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Order NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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Order NEBNext® Quick Ligation Module

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Order NEBNext® Ultra™ End Repair/dA-Tailing Module

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Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Module

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Order NEBNext® Ultra™ Ligation Module

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Order NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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Order NEBNext® DNA Library Prep Master Mix Set for Illumina®

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Order NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra™ II Ligation Module 

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Order NEBNext® Ultra™ II Q5® Master Mix

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Order NEBNext® Ultra™ II End Repair/dA-Tailing Module

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra II RNA First Strand Synthesis Module

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Order NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Ion Torrent™ DNA Library Preparation

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Order NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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Order NEBNext® Fast DNA Library Prep Set for Ion Torrent™

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Order NEBNext® FFPE DNA Repair Mix

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

454™ Library Preparation

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Order NEBNext® End Repair Module

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® Quick Ligation Module

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Order NEBNext® DNA Library Prep Master Mix Set for 454™

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Order NEBNext® End Repair Module

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Order NEBNext® Quick Ligation Module

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Order NEBNext® DNA Library Prep Master Mix Set for 454™

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

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Order NEBNext Direct® BRCA1/BRCA2 Panel

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Order NEBNext Direct® Cancer HotSpot Panel

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Order NEBNext® dA-Tailing Reaction Buffer

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Order NEBNext® End Repair Reaction Buffer

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Order NEBNext® FFPE DNA Repair Mix

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Order NEBNext® High-Fidelity 2X PCR Master Mix

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Order NEBNext® Library Dilution Buffer

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Order NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order NEBNext® Quick Ligation Reaction Buffer

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Order NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Module

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Order NEBNext® Ultra™ II Ligation Module 

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Order NEBNext® Ultra™ II Q5® Master Mix

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Order NEBNext® Ultra™ II End Repair/dA-Tailing Module

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Order NEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

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Order 5´ DNA Adenylation Kit

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Order NEBNext® Library Dilution Buffer

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Order NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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Order NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

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Order NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II RNA First Strand Synthesis Module

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Order NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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Order NEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

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Order NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Module

FFPE DNA Repair

NEBNext® Automation

Illumina® Library Preparation

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Order NEBNext Direct® BRCA1/BRCA2 Panel

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Order NEBNext Direct® Cancer HotSpot Panel

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Order NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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Order NEBNext® dA-Tailing Module

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Order NEBNext® End Repair Module

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Order NEBNext® FFPE DNA Repair Mix

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Order NEBNext® Library Dilution Buffer

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Close

Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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Order NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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Order NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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Order NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order NEBNext® Quick Ligation Module

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Order NEBNext® RNA First Strand Synthesis Module

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

Close

Order NEBNext® Singleplex Oligos for Illumina®

Close

Order NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Close

Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Close

Order NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Close

Order NEBNext® Ultra™ End Repair/dA-Tailing Module

Close

Order NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Module

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Order NEBNext® Ultra™ II Ligation Module 

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Order NEBNext® Ultra™ II Q5® Master Mix

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Order NEBNext® Ultra™ II End Repair/dA-Tailing Module

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Order NEBNext® Ultra™ Ligation Module

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Order NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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Order NEBNext® DNA Library Prep Master Mix Set for Illumina®

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Order NEBNext® Library Quant Kit for Illumina®

RNA for Illumina®

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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Order NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

Close

Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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Order NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® RNA First Strand Synthesis Module

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Order NEBNext® Singleplex Oligos for Illumina®

Close

Order NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Close

Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Close

Order NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Close

Order NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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Order NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

DNA for Illumina®

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Order NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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Order NEBNext® dA-Tailing Module

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Order NEBNext® End Repair Module

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Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

Close

Order NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

Close

Order NEBNext® Quick Ligation Module

Close

Order NEBNext® Ultra™ End Repair/dA-Tailing Module

Close

Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

Close

Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra II FS DNA Module

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Order NEBNext® Ultra™ Ligation Module

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Order NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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Order NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® Ultra™ II DNA Library Prep

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Order NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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Order NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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Order NEBNext® Ultra™ II Ligation Module 

Close

Order NEBNext® Ultra™ II Q5® Master Mix

Close

Order NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Ultra™ II RNA Library Prep

Close

Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Close

Order NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Close

Order NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

Close

Order NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

Close

Order NEBNext® Ultra II RNA First Strand Synthesis Module

Close

Order NEBNext® Ultra II RNA Library Prep Kit for Illumina®

Close

Order NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Ion Torrent™ DNA Library Preparation

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Order NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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Order NEBNext® Fast DNA Library Prep Set for Ion Torrent™

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Order NEBNext® FFPE DNA Repair Mix

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

DNA for Ion Torrent™

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Order NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

Close

Order NEBNext® Fast DNA Library Prep Set for Ion Torrent™

Close

Order NEBNext® FFPE DNA Repair Mix

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

454™ Library Preparation

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Order NEBNext® End Repair Module

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® Quick Ligation Module

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Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Order NEBNext® DNA Library Prep Master Mix Set for 454™

DNA for 454™

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Order NEBNext® End Repair Module

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Order NEBNext® Quick Ligation Module

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Order NEBNext® DNA Library Prep Master Mix Set for 454™

RNA for 454™

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Order NEBNext® Magnesium RNA Fragmentation Module

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Order NEBNext® Poly(A) mRNA Magnetic Isolation Module

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

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Order NEBNext Direct® BRCA1/BRCA2 Panel

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Order NEBNext Direct® Cancer HotSpot Panel

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Order NEBNext® dA-Tailing Reaction Buffer

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Order NEBNext® End Repair Reaction Buffer

Close

Order NEBNext® FFPE DNA Repair Mix

Close

Order NEBNext® High-Fidelity 2X PCR Master Mix

Close

Order NEBNext® Library Dilution Buffer

Close

Order NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Close

Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

Close

Order NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

Close

Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

Order NEBNext® Quick Ligation Reaction Buffer

Close

Order NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Close

Order NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

Close

Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

Close

Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

Close

Order NEBNext® Ultra II FS DNA Module

Close

Order NEBNext® Ultra™ II Ligation Module 

Close

Order NEBNext® Ultra™ II Q5® Master Mix

Close

Order NEBNext® Ultra™ II End Repair/dA-Tailing Module

Close

Order NEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

Close

Order 5´ DNA Adenylation Kit

Close

Order NEBNext® Library Dilution Buffer

Close

Order NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Close

Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

Close

Order NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

Close

Order NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

Close

Order NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Close

Order NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

Close

Order NEBNext® Ultra II RNA First Strand Synthesis Module

Close

Order NEBNext® Ultra II RNA Library Prep Kit for Illumina®

Close

Order NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Close

Order NEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

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Order NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

Close

Order NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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Order NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

Close

Order NEBNext® Ultra II FS DNA Module

FFPE DNA Repair

NEBNext FFPE DNA Repair Mix

NEBNext® Automation