Ion Torrent™ DNA Library Preparation

In general, library preparation of fragmented DNA, for use with the Ion Torrent™ sequencing platform, involves repair of 3′ and 5′ ends before ligation to an adaptor. After ligation, adaptor-DNA constructs are purified and size selected, and amplified via polymerase chain reaction (PCR). For more information about DNA Library Construction, choose the workflow tabs below.

Libraries prepared in this manner are suitable for template preparation, the next step in the Ion Torrent sequencing workflow.

NEBNext® reagents are available for each step in the DNA library preparation workflow for the Ion Torrent platform. For DNA library preparation, reagents from NEB are available with or without reagents for enzyme-based fragmentation. Each of these reagents has been functionally validated by preparation of a genomic DNA library followed by Ion Torrent sequencing. For representative results of both library construction using NEBNext and Ion Torrent run results, choose the tabs below.

Ion Torrent™ is a trademark owned by Life Technologies, Inc.

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Ion Torrent™ DNA Library Preparation includes these areas of focus:

DNA for Ion Torrent™

Protocols for Ion Torrent™ DNA Library Preparation

    Other Tools & Resources


  • NEBNext for Ion Torrent™ Brochure

    The NEBNext Brochure for Ion Torrent provides an overview of library preparation for DNA for the Ion Torrent platform, as well as useful technical tips.

  • The NEBNext Sample Preparation Workflow Poster

    The NEBNext Sample Preparation Workflow Overviews & Products poster gives you at-a-glance workflow details for our DNA & RNA library prep products for Illumina & Ion Torrent, as well as our latest featured products.

DNA Preparation Workflow for Ion Torrent

Representative Results from Libraries Constructed Using NEBNext for Ion Torrent

Representative Ion Torrent Run Report