Next Generation Sequencing
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  • Ion Torrent™ DNA Library Preparation

    In general, library preparation of fragmented DNA, for use with the Ion Torrent™ sequencing platform, involves repair of 3’ and 5’ ends before ligation to an adaptor. After ligation, adaptor-DNA constructs are purified and size selected, and amplified via polymerase chain reaction (PCR). For more information about DNA Library Construction, choose the workflow tabs below.

    Libraries prepared in this manner are suitable for template preparation, the next step in the Ion Torrent sequencing workflow.

    NEBNext® reagents are available for each step in the DNA library preparation workflow for the Ion Torrent platform. For DNA library preparation, reagents from NEB are available with or without reagents for enzyme-based fragmentation. Each of these reagents has been functionally validated by preparation of a genomic DNA library followed by Ion Torrent sequencing. For representative results of both library construction using NEBNext and Ion Torrent run results, choose the tabs below.

    Ion Torrent™ is a trademark owned by Life Technologies, Inc.
    NEBNext® is a registered trademark of New England Biolabs, Inc.
    1. Which DNA Library Prep Kit for Ion Torrent Should I Choose?

      Having trouble deciding which NEBNext Fast DNA Library Prep Set for Ion Torrent™ to choose? Let Christine explain which set is right for your DNA.

    2. NEBNext for Core Lab Facilities

      Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs.

    3. Why Choose NEBNext Reagents for NGS Library Prep?

      NEBNext products are a great choice for your NGS library preparation needs. In this video, Fiona shares a few reasons why.

    4. 12 Quick Tips for NGS Library Preparation

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    5. Tips for Bead-based Clean ups and Size Selection

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    6. Tips for Optimizing DNA Inputs in NGS Library Construction

      Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

    Featured Products

    Ion Torrent™ DNA Library Preparation includes these areas of focus:

    DNA for Ion Torrent™

    Protocols for Ion Torrent™ DNA Library Preparation

    DNA Library Construction Workflow for Ion Torrent

    DNA Preparation Workflow for Ion Torrent

    Representative Results from Libraries Constructed Using NEBNext for Ion Torrent

    Representative Ion Torrent Run Report