In general, library preparation of fragmented DNA and ChIP-Seq DNA for use with the Illumina® sequencing platform involves repair of 3’ and 5’ ends to form blunt-ended, phosphorylated molecules, and the addition of a non-templated dA-tail before ligation to an adaptor. The final step in both workflows is amplification via polymerase chain reaction (PCR). For more information, choose the workflow tab below.
Libraries prepared in this manner are suitable for cluster generation, the next step in the Illumina sequencing workflow.
NEBNext® reagents are available for each step in the DNA and ChIP-Seq library preparation workflows for the Illumina series of instruments. Reagents from NEB are available in a set, master mix or module format. Each of these reagents has been functionally validated by preparation of a genomic DNA or ChIP-Seq DNA library, followed by Illumina sequencing.
Illumina® is a registered trademark of Illumina, Inc.
NEBNext® is a registered trademark of New England Biolabs, Inc.