Next Generation Sequencing
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  • DNA for Illumina®

    In general, library preparation of fragmented DNA and ChIP-Seq DNA for use with the Illumina® sequencing platform involves repair of 3’ and 5’ ends to form blunt-ended, phosphorylated molecules, and the addition of a non-templated dA-tail before ligation to an adaptor. The final step in both workflows is amplification via polymerase chain reaction (PCR). For more information, choose the workflow tab below.

    Libraries prepared in this manner are suitable for cluster generation, the next step in the Illumina sequencing workflow.

    NEBNext® reagents are available for each step in the DNA and ChIP-Seq library preparation workflows for the Illumina series of instruments. Reagents from NEB are available in a set, master mix or module format. Each of these reagents has been functionally validated by preparation of a genomic DNA or ChIP-Seq DNA library, followed by Illumina sequencing.

    Illumina® is a registered trademark of Illumina, Inc.
    NEBNext® is a registered trademark of New England Biolabs, Inc.


    • Optimization of NGS Library Preparation: Low Inputs and Fast, Streamlined Workflows

      Learn how NEBNext® Ultra is enabling library prep for multiple applications, with lower input amounts and fast, streamlined workflows. In this GenomeWeb webinar Cynthia Hendrickson from HudsonAlpha Institute of Biotechnology, Momo Vuyisich from Los Alamos National Laboratory and Daniela Munafo from New England Biolabs describe use of NEBNext Ultra in exome sequencing, bacterial genome resequencing and assembly, and strand-specific RNA-Seq.

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    FAQs for DNA for Illumina®

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      Brochures

    • NEBNext® for Illumina® Brochure

      The NEBNext Brochure for Illumina provides an overview of library preparation for DNA, ChIP-Seq, RNA and Small RNA for the Illumina platform, as well as tech tips and select peer-reviewed citations.

    DNA Library Preparation Workflow for Illumina

    ChIP DNA Library Preparation Workflow for Illumina

    Ultra DNA Library Preparation for Illumina

    DNA Product Details for Illumina