Next Generation Sequencing

DNA for Illumina®

In general, library preparation of fragmented DNA and ChIP-Seq DNA for use with the Illumina® sequencing platform involves repair of 3’ and 5’ ends to form blunt-ended, phosphorylated molecules, and the addition of a non-templated dA-tail before ligation to an adaptor. The final step in both workflows is amplification via polymerase chain reaction (PCR). For more information, choose the workflow tab below.

Libraries prepared in this manner are suitable for cluster generation, the next step in the Illumina sequencing workflow.

NEBNext® reagents are available for each step in the DNA and ChIP-Seq library preparation workflows for the Illumina series of instruments. Reagents from NEB are available in a set, master mix or module format. Each of these reagents has been functionally validated by preparation of a genomic DNA or ChIP-Seq DNA library, followed by Illumina sequencing.

Illumina® is a registered trademark of Illumina, Inc.
NEBNext® is a registered trademark of New England Biolabs, Inc.


  1. Optimization of NGS Library Preparation: Low Inputs and Fast, Streamlined Workflows

    Learn how NEBNext® Ultra™ is enabling library prep for multiple applications, with lower input amounts and fast, streamlined workflows. In this GenomeWeb webinar Cynthia Hendrickson from HudsonAlpha Institute of Biotechnology, Momo Vuyisich from Los Alamos National Laboratory and Daniela Munafo from New England Biolabs describe use of NEBNext Ultra in exome sequencing, bacterial genome resequencing and assembly, and strand-specific RNA-Seq.

Featured Products

FAQs for DNA for Illumina®

Protocols for DNA for Illumina®

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

DNA Library Preparation Workflow for Illumina

ChIP DNA Library Preparation Workflow for Illumina

Ultra II DNA Library Preparation for Illumina

DNA Product Details for Illumina