DNA for Illumina®

Library preparation for the Illumina sequencing platform requires fragmentation of DNA followed by repair of 3’ and 5’ ends to form blunt-ended, phosphorylated molecules, and the addition of a non-templated dA-tail before ligation to an adaptor. If necessary to achieve sufficient yields, the final step is PCR amplification of the library. For more detail, select a workflow tab below.

NEBNext® kits are available for sample preparation of genomic DNA, ChIP DNA and FFPE DNA. Our most recent NEBNext Ultra II FS DNA kits include a new fragmentation reagent, which is also combined with end repair and dA-tailing reagents, enabling these steps to be performed in the same tube, without any clean-ups (and therefore without any sample loss). The same fragmentation protocol is used for any input amount from 100 pg to 500 ng, and for any GC content DNA. The high-efficiency Ultra II ligation reagents and Q5 Ultra II PCR master mix complete the library prep workflow. The result is a fast, high-yield, high-quality library prep workflow that is completely scalable. 

FAQs for DNA for Illumina®

Protocols for DNA for Illumina®

Ultra II FS DNA Library Preparation Workflow

ChIP DNA Library Preparation Workflow for Illumina

Ultra II DNA Library Preparation for Illumina

DNA Product Details for Illumina

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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