ChIP-Seq DNA Library Preparation

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Preparation methods for Chomatin-immunoprecipitated (ChIP-Seq) DNA libraries are similar to those for standard DNA. However, the amount of input DNA for ChIP-Seq libraries is lower than for standard DNA library construction, and library preparation requires the use of optimized reagents and protocols. Choose the ChIP-Seq Library Construction Workflow tab below to see specific workflows for the preparation of ChIP-Seq libraries for both Illumina and SOLiD sequencing. The isolated ChIP-Seq DNA is treated to remove overhangs and add 5’ phosphates and 3’ hydroxyls (Figure 1). Libraries to be used with the SOLiD™ 4 platform can be directly ligated to adaptor after end repair and size selection. For Illumina® and Quick 454™ libraries, there is an addition of a dA-tail before ligation to an adaptor. Libraries are then amplified by PCR.

Figure 1: ChIP-Seq Library Construction Workflow

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