NEBNext® dsDNA Fragmentase® is an enzyme-based solution used for the fragmentation of DNA. dsDNA Fragmentase generates 100-800bp fragments in a time dependent manner. The solution contains two enzymes; one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl groups. This nicking activity has been shown to be random. Comparison of sequencing data from libraries prepared with DNA sheared mechanically or by dsDNA Fragmentase (Figure 1) demonstrates that the dsDNA Fragmentase does not introduce any detectable bias. In addition, no difference in sequence coverage is observed between these methods. dsDNA Fragmentase can be used with genomic DNA, PCR products and whole genome amplified DNA, of any degree of GC content.
NEBNext® and Fragmentase® are registered trademarks of New England Biolabs, Inc.