Applications of Deglycosylation

Return to Removal of N-Linked & O-Linked Glycans from Glycoproteins

There are a number of reasons to deglycosylate a glycoprotein, including:

  • To remove heterogeneity in glycoproteins for X-ray crystallographic analysis
  • To remove carbohydrate epitopes from antigens
  • To enhance or reduce blood clearance rates of glycoprotein therapeutics
  • To investigate the role of carbohydrates in enzyme activity and solubility
  • To investigate ligand binding
  • For quality control of glycoprotein pharmaceuticals
  • To simplify analysis of the peptide portion of the glycoprotein
  • To simplify the analysis of the glycan component via glycan sequencing