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  • Applications of Deglycosylation

    Return to Removal of N-Linked & O-Linked Glycans from Glycoproteins

    There are a number of reasons to deglycosylate a glycoprotein, including:

    • To remove heterogeneity in glycoproteins for X-ray crystallographic analysis
    • To remove carbohydrate epitopes from antigens
    • To enhance or reduce blood clearance rates of glycoprotein therapeutics
    • To investigate the role of carbohydrates in enzyme activity and solubility
    • To investigate ligand binding
    • For quality control of glycoprotein pharmaceuticals
    • To simplify analysis of the peptide portion of the glycoprotein
    • To simplify the analysis of the glycan component via glycan sequencing