Heparin and heparan sulfate (HS) glycosaminoglycans are linear sulfated polysaccharides located on cell-surface membranes and in extracellular matrices in virtually all animal tissues. Heparin and HS have been implicated in cell-biological processes, cell adhesion and regulation of enzymatic catalysis (1). HS chains have been shown to interact with a variety of growth factors, chemokines, extracellular matrix proteins, and enzymes, including antithrombin, fibroblast growth factors, and vascular endothelial growth factor (2) as shown by choosing the tab below. Heparin has been widely used as an anticoagulant drug (3,4), and it has been shown to regulate cellular process by binding, stabilizing and activating various growth factors (5).
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Depolymerization of Heparin/HS includes these areas of focus:
HS chain interactions
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Learn more about the structural elucidation of complex and diverse heparin oligosaccharides using Bacteroides Heparinase I, II and III in combination with downstream mass spec analysis.
Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video. Analysis of these glycans can be accomplished with the use of deglycosylation enzymes, which can provide complete sugar removal with no protein degradation.
Learn how glycosidases are used to analyze multiple glycoprotein samples. Here, the model glycoprotein, hCGβ, which carries both N- and O-glycans, is demonstrated.