DNA Methylation Analysis

Methods to analyze DNA methylation, DNA demethylation, and their functional effects are critical to epigenetics researchers. Methylation profiles of epigenome are used for disease identification and for research and therapeutic development. Analysis can be gene-specific or global depending on downstream applications (1).

Downstream methylation detection methods include end-point PCR, real-time PCR, primer extension, single-stranded conformational polymorphism assays, blotting, microarray analysis and sequencing. Choosing which method(s) to use largely depends on the experimental sample size and the goals of the experiments.

 
  1. Tao Zuo et al. 2009, Epigenomics  1(2) 331-345. PMID: 20526417

DNA Methylation Analysis includes these areas of focus:

5-hmC Detection & Analysis
Methylated DNA Analysis
Bisulfite Conversion

FAQs for DNA Methylation Analysis

Protocols for DNA Methylation Analysis

    Publications related to DNA Methylation Analysis

  1. Hughes J.R., Roberts N., McGowan S., Hay D., Giannoulatou E., Lynch M., De Gobbi M., Taylor S., Gibbons R., Higgs D.R. 2014. Analysis of hundreds of cis-regulatory landscapes at high resolution in a single, high-throughput experiment Nat Genet. 46 (2), PubMedID: 24413732, DOI: doi:10.1038/ng.2871
  2. Sexton T, Kurukuti S, Mitchell JA, Umlauf D, Nagano T, Fraser P 2012. Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip Nat Protoc. 7(7), PubMedID: 22722369, DOI: 10.1038/nprot.2012.071

Methods of DNA Methylation Analysis

  • Bisulfite conversion, which provides single nucleotide, high resolution data for whole genomes or single locus methylation studies.
  • Differential enzymatic cleavage, which uses methylation, or dually 5-mC and 5-hmC dependent enzymes, paired with non-sensitive isoschizomers to asses DNA methylation or DNA hydroxymethylation.
  • High-Performance Liquid Chromatography (HPLC)
  • Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry(MALDI-TOF MS)
  • Methylated DNA-ELISA
  • Affinity-based methods, which use methylated DNA binding proteins or antibodies for enrichment.
  • Enzymatic DNA methyltransferase assay

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  1. What is Epigenetics?

    What Is Epigenetics?

    If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.