DNA Modification

DNA Ligation

Ligation of DNA is a central step in many modern molecular biology workflows. DNA ligases catalyze the formation of a phosphodiester bond between the 3′ hydroxyl and 5′ phosphate of adjacent DNA residues. In living organisms, ligases are essential enzymes with critical roles in DNA replication and repair. In the lab, this reaction is used to join dsDNA fragments with blunt or cohesive ends to form recombinant DNA plasmids, to add bar-coded adapters to fragmented DNA during next-generation sequencing, and to join DNA probes for SNP detection using the ligase chain reaction (LCR).

Protocols for DNA Ligation

Common Applications of Exonucleases and Endonucleases

NEB provides a list of common applications for our exonucleases and endonucleases.

Properties of Exonucleases and Endonucleases

NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. DNA Ligase

    DNA Ligation

    Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about ligation with our quick animation.