DNA Modification

DNA Phosphorylation

Single- or double-stranded DNA with a 5'-hydroxyl terminus has to be phosphorylated prior to ligation as 5’ phosphate groups are required for ligation. Phosporylation of DNA ends with phosphate radioisotopes can also be used to label DNA in preparation of DNA for subsequent detection, isolation and sequencing applications. A number of polynucleotide kinases, including T4 PNK (NEB #M0201) and T4 PNK (3' phosphatase minus) (NEB #M0236), can be used to transfer the γ-phosphate of ATP to a 5’ terminus of DNA.

  1. The Mechanism of DNA Phosphorylation

    Phosphorylation is the process by which phosphate groups are added to a molecule by a kinase. The phosphorylation status of a fragment of DNA can influence its ability to proceed in reactions. Learn more about phosphorylation and kinases.

Featured Products

Protocols for DNA Phosphorylation

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Common Applications of Exonucleases and Endonucleases

NEB provides a list of common applications for our exonucleases and endonucleases.

Properties of Exonucleases and Endonucleases

NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.