DNA Modification

DNA Modification

The preparation of DNA for molecular applications often requires sample processing beyond restriction enzyme digestion, fragmentation or PCR amplification. Modifying enzymes can be used to manipulate or repair DNA for downstream applications, such as:

  • Recombinant DNA assembly for subsequent cloning
  • PCR amplification
  • DNA labeling or probing
  • Sequencing

While there are many modifying enzymes that act on DNA to prepare it for a variety of different downstream applications, there are also modifying enzymes that are used to study genomic DNA methylation as a method of epigenetic change.

Featured Products

DNA Modification includes these areas of focus:

DNA Nicking
DNA End Modification
DNA Blunting
DNA Phosphorylation
DNA Dephosphorylation
DNA Repair
DNA & Protein Methylation
DNA Ligation

Protocols for DNA Modification

Common Applications of Exonucleases and Endonucleases

NEB provides a list of common applications for our exonucleases and endonucleases.

Properties of Exonucleases and Endonucleases

NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.