The preparation of DNA for molecular applications often requires sample processing beyond restriction enzyme digestion, fragmentation or PCR amplification. Modifying enzymes can be used to manipulate or repair DNA for downstream applications, such as:
- Recombinant DNA assembly for subsequent cloning
- PCR amplification
- DNA labeling or probing
While there are many modifying enzymes that act on DNA to prepare it for a variety of different downstream applications, there are also modifying enzymes that are used to study genomic DNA methylation as a method of epigenetic change.