DNA Amplification

Long Range PCR

Long Range PCR refers to the amplification of DNA lengths that cannot typically be amplified using routine PCR methods or reagents. For simple DNA templates, polymerases optimized for Long Range PCR can amplify up to 30 kb and beyond. For complex, genomic templates, 20 kb is a typical target.

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Protocols for Long Range PCR
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    Tips for Amplifying Large Amplicons

    Do you need to optimize your PCR of large amplicons? Becky shares some tips for choosing the right polymerase and cycler conditions, as well as optimal handing methods, to get the most out of those long templates!