The ability to synthesize DNA from an RNA template, via reverse transcription, enables researchers to study RNA with the same molecular approaches used for DNA investigations. cDNA generated by reverse transcription can be amplified using polymerase chain reaction (PCR). The combination of reverse transcription and PCR (RT-PCR) permits sensitive detection of low abundance RNAs in a sample. The resultant PCR Products can be used in many downstream applications, such as cloning into plasmid vectors and sequencing using next generation sequencing platforms.

In order to learn more about our RT-PCR products, please visit our product overview.

Protocols for RT-PCR

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.