Routine PCR
Choose Type:
FAQs for Routine PCR
Protocols for Routine PCR
- Protocol for OneTaq 2X Master Mix with GC Buffer (M0483)
- Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (M0488)
- OneTaq® Quick-Load® 2X Master Mix with GC Buffer (M0487)
- PCR Protocol Phusion® DNA Polymerase
- Protocol for a Routine Deep Vent® PCR
- Protocol for a Routine Taq PCR
- Taq DNA Polymerase Guidelines for PCR Optimization
- PCR Protocol for Taq DNA Polymerase
- PCR Protocol for Taq DNA Polymerase with Standard Taq (Mg-free) Buffer (M0320)
- PCR Protocol for Taq DNA Polymerase with ThermoPol Buffer (M0267)
- PCR Protocol for LongAmp® Hot Start Taq DNA Polymerase (M0534)
- Protocol for LongAmp™ Taq PCR Kit
- Protocol for Phusion® Hot Start Flex 2X Master Mix
- PCR Using NEBNext® High-Fidelity 2X PCR Master Mix (M0541)
- PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493)
- Protocol for Q5® Hot Start High-Fidelity 2X Master Mix
- Protocol for OneTaq Hot Start DNA Polymerase (M0481)
- Protocol for LongAmp™ Taq 2X Master Mix
- Guidelines for PCR Optimization for Deep Vent® DNA Polymerase
- Guidelines for PCR Optimization for Vent DNA Polymerase
- Protocol for a Routine Deep Vent® (exo-) PCR
- Protocol for a Routine Vent PCR
- Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)
- Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer
- PCR Protocol for Phusion® Hot Start Flex DNA Polymerase (M0535)
- Protocol Phusion® High-Fidelity PCR Master Mix with HF Buffer
- Protocol for OneTaq Hot Start 2X Master Mix with GC Buffer (M0485)
- Protocol for OneTaq Hot Start 2X Master Mix with Standard Buffer (M0484)
- PCR Optimization with Phusion® High-Fidelity PCR Kit
- Protocol for a Routine PCR with Phusion® High-Fidelity PCR Kit
- Protocol for a Routine Vent (exo-) PCR
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Protocol for OneTaq® Quick-Load 2X Master Mix with Standard Buffer (M0486)
- PCR Optimization of the Control Template using Phusion® High-Fidelity PCR Kit
- Protocol for Phusion® High-Fidelity PCR Master Mix with GC Buffer
- Protocol for Quick-Load® Taq 2X Master Mix
- Protocol for Taq 2X Master Mix (M0270)
- Protocol for Taq 5X Master Mix (M0285)
- EpiMark® Hot Start Taq DNA Polymerase Guidelines for PCR (M0490)
- Protocol for a PCR reaction using Hot Start Taq 2X Master Mix (M0496)
- PCR Using Hot Start Taq DNA Polymerase (M0495)
- PCR Protocol for OneTaq® DNA Polymerase (M0480)
- PCR Protocol for LongAmp® Taq DNA Polymerase (M0323)
- Protocol for LongAmp™ Hot Start Taq 2X Master Mix
- Protocol for Q5® High-Fidelity 2X Master Mix
- PCR Optimization (E0555)
- Protocol for a Routine PCR (E0555)
- A-Tailing with Taq Polymerase
- PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
- Loop-mediated Isothermal Amplification (LAMP)
- Protocol for a PCR reaction using NEBNext® Q5® Hot Start HiFi PCR Master Mix (M0543)
-
Understanding Variability in DNA Amplification Reactions
-
Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
-
Polymerase Fidelity: What is it, and what does it mean for your PCR?
- PCR Reagents Brochure
- PCR Selector
- Polbase
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Tools & Resources
Feature Articles
Brochures
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Vladimir Potapov, Jennifer L. Ong. (2017) Examining Sources of Error in PCR by Single-Molecule Sequencing. PLOS One; PubMedID: 28683110
- Megan S Thoemmes, Daniel J Fergus, Julie Urban, Michelle Trautwein, Robert R Dunn (2014) Ubiquity and diversity of human-associated demodex mites. PLoS One; 9, e106265. PubMedID: 25162399, DOI: 10.1371/journal.pone.0106265
Publications related to Routine PCR
Legal Information
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
