: Extraction-Free or Direct PCR describes amplification from blood, soil or other complex samples without an initial purification step. The presence of inhibitors in these sample types can reduce successful amplification. Optimized polymerase formulations can enable robust Extraction-Free PCR experiments. For example, Hemo KlenTaq can tolerate up to 20% whole blood added directly to a PCR mixture.
Next Generation Sequencing Library Construction
: Minimizing bias is important for even amplification of NGS libraries including GC-rich and AT-rich amplicons. NEBNext® High-Fidelity 2X PCR Master Mix (M0541) combines high fidelity and robust, even amplification making it an ideal choice for your NGS library construction.
: Multiplex PCR involves the simultaneous amplification of two or more primer sets in a single reaction. Specialized enzyme formulations can also increase multiplex performance and reduce the need for troubleshooting. NEB offers both Taq-based and Q5-based formulations
that are appropriate for use in multiplex PCR.