DNA Amplification
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  • High-Fidelity PCR

    The fidelity of a polymerase refers to its ability to insert the correct base during PCR. Conversely, the rate of misincorporation is known as a polymerase’s error rate. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. NEB scientists were the first to identify and commercialize a high-fidelity DNA polymerase suitable for PCR, namely VentR® 

    DNA Polymerase.Since its discovery, several other high fidelity polymerases have been discovered and engineered – all with differing processivity, speed and levels of accuracy..

    When do you need a high-fidelity DNA polymerase?

    1. How Can I Improve Amplification of GC-rich Regions

      The secondary structures that form within GC-rich regions can make efficient DNA amplification difficult. Nicole’s suggestions can help!

    2. Choose the Right DNA Polymerase for PCR

      Make sure you're using the optimal polymerase for your DNA amplifications. Get tips on choosing the right DNA Polymerase for your application.

    3. DNA Replication with a Proofreading Polymerase

      Learn how proofreading polymerases recognize and correct mismatched bases.

    4. How to Amplify GC-rich DNA

      Looking for tips on dealing with GC-bias in DNA amplification? NEB scientists have the expertise you need!

    5. Important Tips for Q5 Polymerase

      Here are some quick tips for getting the most out of NEB's Q5® High-Fidelity DNA Polymerase.

    6. Why Choose Q5 High-fidelity Polymerase?

      Not sure why Q5® is your best choice for high-fidelity amplification of GC-rich targets? NEB's scientists will show you why we call Q5 an "ultra-high fidelity polymerase".

    FAQs for High-Fidelity PCR

    Protocols for High-Fidelity PCR

      Publications related to High-Fidelity PCR:

    1. Yonghe Zhang, Huiming Huang, Shanshan Xu, Bo Wang, Jianhua Ju, Huarong Tan, Wenli Li (2015). Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact. 14, 64. PubMedID: 25927229, DOI: 10.1186/s12934-015-0244-2
    2. Binyamin D Berkovits, Christine Mayr (2015). Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature. , PubMedID: 25896326, DOI: 10.1038/nature14321
    3. Jun Wu, Daiji Okamura, Mo Li, Keiichiro Suzuki, Chongyuan Luo, Li Ma, Yupeng He, Zhongwei Li, Chris Benner, Isao Tamura, Marie N Krause, Joseph R Nery, Tingting Du, Zhuzhu Zhang, Tomoaki Hishida, Yuta Takahashi, Emi Aizawa, Na Young Kim, Jeronimo Lajara, Pedro Guillen, Josep M Campistol, Concepcion Rodriguez Esteban, Pablo J Ross, Alan Saghatelian, Bing Ren, Joseph R Ecker, Juan Carlos Izpisua Belmonte (2015). An alternative pluripotent state confers interspecies chimaeric competency. Nature. , PubMedID: 25945737, DOI: 10.1038/nature14413
    4. Longhai Dai, Can Liu, Yueming Zhu, Jiangsheng Zhang, Yan Men, Zeng Yan, Yuanxia Sun (2015). Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii. Plant Cell Physiol. , PubMedID: 25759326, DOI: 10.1093/pcp/pcv043
    5. Amin Zargar, David N Quan, Milad Emamian, Chen Yu Tsao, Hsuan-Chen Wu, Chelsea R Virgile, William E Bentley (2015). Rational design of 'controller cells' to manipulate protein and phenotype expression. Metab Eng. , PubMedID: 25908186, DOI: 10.1016/j.ymben.2015.04.001
    6. Christine Henke, Pamela L Strissel, Maria-Theresa Schubert, Megan Mitchell, Claus C Stolt, Florian Faschingbauer, Matthias W Beckmann, Reiner Strick (2015). Selective expression of sense and antisense transcripts of the sushi-ichi-related retrotransposon - derived family during mouse placentogenesis. Retrovirology. 12, 9. PubMedID: 25888968, DOI: 10.1186/s12977-015-0138-8
    7. Harish Nag Kankipati, Marta Rubio-Texeira, Dries Castermans, George Diallinas, Johan M Thevelein (2015). Sul1 and Sul2 Sulfate Transceptors Signal to Protein Kinase A upon Exit of Sulfur Starvation. J Biol Chem. 290, 10430-46. PubMedID: 25724649, DOI: 10.1074/jbc.M114.629022
    8. Yuan Xue, Jossef Osborn, Anand Panchal, Jay L Mellies (2015). The RpoE Stress Response Pathway Mediates Reduction of the Virulence of Enteropathogenic Escherichia coli by Zinc. Appl Environ Microbiol. 81, 3766-74. PubMedID: 25819956, DOI: 10.1128/AEM.00507-15
    9. Yafeng Li, Delu Song, Ying Song, Liangliang Zhao, Natalie Wolkow, John W Tobias, Wenchao Song, Joshua L Dunaief (2015). Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-β Signaling in the Retinal Pigment Epithelium. J Biol Chem. 290, 11918-34. PubMedID: 25802332, DOI: 10.1074/jbc.M115.645903
    10. Bert De Rybel, Milad Adibi, Alice S. Breda, Jos R. Wendrich, Margot E. Smit, Ondej Novk, Nobutoshi Yamaguchi, Saiko Yoshida, Gert Van Isterdael, Joakim Palovaara, Bart Nijsse, Mark V. Boekschoten, Guido Hooiveld, Tom Beeckman, Doris Wagner, Karin Ljung, Christian Fleck, Dolf Weijers (2014). Integration of growth and patterning during vascular tissue formation in Arabidopsis Science. 345, 1255215. PubMedID: 25104393, DOI: 10.1126/science.1255215
    11. Vidhyadhar Nandana, Sushant Singh, Abhay Narayan Singh, Vikash Kumar Dubey (2014). Procerain B, a cysteine protease from Calotropis procera, requires N-terminus pro-region for activity: cDNA cloning and expression with pro-sequence. Protein Expr Purif. 103C, 16-22. PubMedID: 25173974, DOI: 10.1016/j.pep.2014.08.003
    12. Martin Kostovcik, Craig C Bateman, Miroslav Kolarik, Lukasz L Stelinski, Bjarte H Jordal, Jiri Hulcr (2014). The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing. ISME J. , PubMedID: 25083930, DOI: 10.1038/ismej.2014.115
    13. Xin Duan, Arjun Krishnaswamy, Irina De la Huerta, Joshua R Sanes (2014). Type II Cadherins Guide Assembly of a Direction-Selective Retinal Circuit. Cell. 158, 793-807. PubMedID: 25126785, DOI: 10.1016/j.cell.2014.06.047