Cloning & Synthetic Biology
Choose Product:
- Cloning & Synthetic Biology
- DNA Analysis
- DNA Assembly and Cloning
- DNA End Modification
- DNA Ligation
- DNA Preparation
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB
- High-throughput cloning and automation solutions
- Nucleic Acid Purification
- Site Directed Mutagenesis
- Transformation
- USER® Cloning
Cloning & Synthetic Biology
DNA Analysis
1 kb DNA Ladder
1 kb Plus DNA Ladder
1 kb Plus DNA Ladder for Safe Stains
100 bp DNA Ladder
50 bp DNA Ladder
5-methyl-dCTP
Acyclonucleotide Set
AleI-v2
BsaI-HF®v2
BsiWI-HF®
BsmBI-v2
BstZ17I-HF®
Deoxynucleotide (dNTP) Solution Mix
Deoxynucleotide (dNTP) Solution Set
Diluent A (with rAlbumin)
Diluent B (with rAlbumin)
Diluent C (with rAlbumin)
DNA Polymerase I, Large (Klenow) Fragment
Fast DNA Ladder
Hot Start Taq DNA Polymerase
Klenow Fragment (3'→5' exo-)
Lambda PFG Ladder
LongAmp® Taq DNA Polymerase
Low Molecular Weight DNA Ladder
MidRange PFG Marker
NEBNext® dA-Tailing Module
NEBNext® End Repair Module
NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™
NEBNext® Fast DNA Library Prep Set for Ion Torrent™
pBR322 DNA-BstNI Digest
pBR322 DNA-MspI Digest
PCR Marker
PsiI-v2
Quick-Load® 1 kb DNA Ladder
Quick-Load® 1 kb Extend DNA Ladder
Quick-Load® 1 kb Plus DNA Ladder
Quick-Load® 100 bp DNA Ladder
Quick-Load® Purple 1 kb DNA Ladder
Quick-Load® Purple 1 kb Plus DNA Ladder
Quick-Load® Purple 100 bp DNA Ladder
Quick-Load® Purple 50 bp DNA Ladder
Quick-Load® Purple Low Molecular Weight DNA Ladder
Recombinant Albumin, Molecular Biology Grade
Standard Taq (Mg-free) Reaction Buffer Pack
Standard Taq Reaction Buffer Pack
Supercoiled DNA Ladder
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
Taq DNA Polymerase with Standard Taq Buffer
Taq DNA Polymerase with ThermoPol® Buffer
ThermoPol® Reaction Buffer
TriDye™ 1 kb DNA Ladder
TriDye™ 1 kb Plus DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ Ultra Low Range DNA Ladder
λ DNA-BstEII Digest
λ DNA-HindIII Digest
λ DNA-Mono Cut Mix
ΦX174 DNA-HaeIII Digest
DNA Assembly and Cloning
BbsI
BbsI-HF®
BsaI-HF®v2
BsmBI-v2
BspQI
EcoRI-HF®
Esp3I
Gibson Assembly® Cloning Kit
Gibson Assembly® Master Mix
Hi-T4™ DNA Ligase
NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2)
NEBridge® Golden Gate Assembly Kit (BsmBI-v2)
NEBridge® Ligase Master Mix
NEBuilder® HiFi DNA Assembly Bundle for Large Fragments
NEBuilder® HiFi DNA Assembly Cloning Kit
NEBuilder® HiFi DNA Assembly Master Mix
PaqCI®
Phusion® High-Fidelity DNA Polymerase
PluTI
PstI
Q5® High-Fidelity DNA Polymerase
Salt-T4® DNA Ligase
SapI
SpeI-HF®
T4 DNA Ligase
T5 Exonuclease
T7 DNA Ligase
Taq DNA Ligase
Thermolabile USER® II Enzyme
Thermostable USER® III Enzyme
USER® Enzyme
XbaI
DNA End Modification
5-hydroxymethyluridine DNA Kinase
Antarctic Phosphatase
Antarctic Phosphatase Reaction Buffer
Deoxynucleotide (dNTP) Solution Set
DNA Polymerase I, Large (Klenow) Fragment
Klenow Fragment (3'→5' exo-)
Mung Bean Nuclease
NEBNext® dA-Tailing Module
NEBNext® dA-Tailing Reaction Buffer
Quick Blunting™ and Quick Ligation™ Kits
Quick Blunting™ Kit
Quick CIP
Shrimp Alkaline Phosphatase (rSAP)
Standard Taq (Mg-free) Reaction Buffer Pack
Standard Taq Reaction Buffer Pack
T4 DNA Ligase Reaction Buffer
T4 DNA Polymerase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (3' phosphatase minus)
T4 Polynucleotide Kinase Reaction Buffer
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
Taq DNA Polymerase with Standard Taq Buffer
Taq DNA Polymerase with ThermoPol® Buffer
Terminal Transferase
ThermoPol® Reaction Buffer
DNA Ligation
9°N™ DNA Ligase
Blunt/TA Ligase Master Mix
dGTP Solution
E. coli DNA Ligase
ElectroLigase®
HiFi Taq DNA Ligase
Hi-T4™ DNA Ligase
Immobilized T4 DNA Ligase
Instant Sticky-end Ligase Master Mix
NEBNext® Quick Ligation Module
NEBuffer™ Set (EcoRI/SspI, DpnII)
Quick Blunting™ and Quick Ligation™ Kits
Quick Ligation™ Kit
Salt-T4® DNA Ligase
StickTogether™ DNA Ligase Buffer
T3 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase Reaction Buffer
T7 DNA Ligase
Taq DNA Ligase
Taq DNA Ligase Reaction Buffer
Thermostable 5´ App DNA/RNA Ligase
DNA Preparation
3´-Biotin-GTP
3´-Desthiobiotin-GTP
5-methyl-dCTP
7-deaza-dGTP
AatII
Acc65I
AccI
AciI
AclI
AcuI
Acyclonucleotide Set
Adenosine 5'-Triphosphate (ATP)
AfeI
AflII
AflIII
AgeI-HF®
AhdI
AleI-v2
AluI
AlwI
AlwNI
AMV Reverse Transcriptase
ApaI
ApaLI
ApeKI
ApoI-HF®
AscI
AseI
AsiSI
AvaI
AvaII
AvrII
BaeGI
BaeI
BamHI
BamHI-HF®
BanI
BanII
BbsI
BbsI-HF®
BbvCI
BbvI
BccI
BceAI
BcgI
BciVI
BclI
BclI-HF
BcoDI
BfaI
BfuAI
BglI
BglII
BlpI
BmgBI
BmrI
BmtI-HF®
BpmI
Bpu10I
BpuEI
BsaAI
BsaBI
BsaHI
BsaI-HF®v2
BsaJI
BsaWI
BsaXI
BseRI
BseYI
BsgI
BsiEI
BsiHKAI
BsiWI
BslI
BsmAI
BsmBI-v2
BsmFI
BsmI
BsoBI
Bsp1286I
BspCNI
BspDI
BspEI
BspHI
BspMI
BspQI
BsrBI
BsrDI
BsrFI-v2
BsrI
BssHII
BssSI-v2
BstAPI
BstBI
BstEII-HF®
BstNI
BstUI
BstYI
Bsu DNA Polymerase, Large Fragment
Bsu36I
BtgI
BtgZI
BtsCI
BtsIMutI
BtsI-v2
Cac8I
ClaI
CspCI
CviKI-1
CviQI
dATP Solution
DdeI
Deep Vent® (exo-) DNA Polymerase
Deep Vent® DNA Polymerase
Deoxynucleotide (dNTP) Solution Mix
Deoxynucleotide (dNTP) Solution Set
DNA Polymerase I (E. coli)
DNA Polymerase I, Large (Klenow) Fragment
DpnI
DpnII
DraI
DraIII-HF®
DrdI
dUTP Solution
E. coli Poly(A) Polymerase
EaeI
EagI-HF®
EarI
EciI
Eco53kI
EcoNI
EcoO109I
EcoP15I
EcoRI
EcoRI-HF®
EcoRV
EcoRV-HF®
EpiMark® Hot Start Taq DNA Polymerase
FatI
FauI
Fnu4HI
FokI
FseI
FspI
HaeII
HaeIII
Hemo KlenTaq®
HgaI
HhaI
HincII
HindIII
HindIII-HF®
HinfI
HinP1I
Hot Start Taq 2X Master Mix
Hot Start Taq DNA Polymerase
HpaI
HpaII
HphI
Hpy166II
Hpy188I
Hpy188III
Hpy99I
HpyCH4III
HpyCH4IV
HpyCH4V
I-CeuI
Induro® Reverse Transcriptase
I-SceI
Isothermal Amplification Buffer II Pack
Isothermal Amplification Buffer Pack
KasI
Klenow Fragment (3'→5' exo-)
KpnI-HF®
LongAmp® Hot Start Taq 2X Master Mix
LongAmp® Hot Start Taq DNA Polymerase
LongAmp® Taq 2X Master Mix
LongAmp® Taq DNA Polymerase
LongAmp® Taq PCR Kit
LunaScript® RT Master Mix Kit (Primer-free)
LunaScript® RT SuperMix
LunaScript® RT SuperMix Kit
M13KO7 Helper Phage
Magnesium Chloride (MgCl2) Solution
Magnesium Sulfate (MgSO4) Solution
MboI
MboII
MfeI-HF®
MluCI
MluI-HF®
MlyI
MmeI
M-MuLV Reverse Transcriptase
MnlI
MscI
MseI
MslI
MspA1I
MspI
MspJI
Multiplex PCR 5X Master Mix
MwoI
NaeI
NarI
NciI
NcoI
NcoI-HF®
NdeI
NEBNext® High-Fidelity 2X PCR Master Mix
NEBNext® Q5® Hot Start HiFi PCR Master Mix
NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module
NEBNext® Ultra™ II Q5® Master Mix
NEBuffer™ 1
NEBuffer™ 2
NEBuffer™ 3
NEBuffer™ 4
NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)
NgoMIV
NheI-HF®
NlaIII
NlaIV
NmeAIII
NotI
NotI-HF®
NruI-HF®
NsiI
NsiI-HF®
NspI
Oligo d(T)18 mRNA Primer
Oligo d(T)23 VN
OneTaq® 2X Master Mix with Standard Buffer
OneTaq® DNA Polymerase
OneTaq® Hot Start 2X Master Mix with GC Buffer
OneTaq® Hot Start 2X Master Mix with Standard Buffer
OneTaq® Hot Start DNA Polymerase
OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer
OneTaq® One-Step RT-PCR Kit
OneTaq® RT-PCR Kit
OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
PacI
PaeR7I
PciI
PflFI
PflMI
Phusion® GC Buffer Pack
Phusion® HF Buffer Pack
Phusion® High-Fidelity DNA Polymerase
Phusion® High-Fidelity PCR Master Mix with GC Buffer
Phusion® High-Fidelity PCR Master Mix with HF Buffer
Phusion® Hot Start Flex 2X Master Mix
Phusion® Hot Start Flex DNA Polymerase
Phusion® High-Fidelity PCR Kit
PI-PspI
PI-SceI
PleI
PmeI
PmlI
Poly(U) Polymerase
PpuMI
PreCR® Repair Mix
ProtoScript® First Strand cDNA Synthesis Kit
ProtoScript® II First Strand cDNA Synthesis Kit
ProtoScript® II Reverse Transcriptase
PshAI
PsiI-v2
PspGI
PspOMI
PspXI
PstI-HF®
PvuI-HF®
PvuII
PvuII-HF®
Q5® High-Fidelity 2X Master Mix
Q5® High-Fidelity DNA Polymerase
Q5® High-Fidelity PCR Kit
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Hot Start High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
Quick Blunting™ Kit
Quick-Load® Taq 2X Master Mix
Random Primer 9
rCutSmart™ Buffer
Recombinant Albumin, Molecular Biology Grade
Ribonucleotide Solution Set
Ribonucleotide Solution Mix
RNase H
RNase Inhibitor, Murine
RsaI
RsrII
SacI-HF®
SacII
S-adenosylmethionine (SAM)
SalI
SalI-HF®
SapI
Sau3AI
Sau96I
SbfI-HF®
ScaI-HF®
ScrFI
SexAI
SfaNI
SfcI
SfiI
SfoI
SgrAI
SmaI
SmlI
SnaBI
SP6 RNA Polymerase
SpeI-HF®
SphI
SphI-HF®
SrfI
SspI-HF®
Standard Taq (Mg-free) Reaction Buffer Pack
Standard Taq Reaction Buffer Pack
StuI
StyD4I
StyI-HF®
Sulfolobus DNA Polymerase IV
SwaI
T3 RNA Polymerase
T4 DNA Polymerase
T4 RNA Ligase 2, truncated KQ
T7 DNA Polymerase (unmodified)
Taq 2X Master Mix
Taq 5X Master Mix
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
Taq DNA Polymerase with Standard Taq Buffer
Taq DNA Polymerase with ThermoPol® Buffer
Taq PCR Kit
TaqI-v2
Template Switching RT Enzyme Mix
Terminal Transferase
TfiI
ThermoPol® Reaction Buffer
Thermostable RNase H
TseI
Tsp45I
TspMI
TspRI
Tth111I
Vent® (exo-) DNA Polymerase
Vent® DNA Polymerase
WarmStart® RTx Reverse Transcriptase
XbaI
XcmI
XhoI
XmaI
XmnI
ZraI
β-Nicotinamide adenine dinucleotide (NAD+)
Fast Cloning: Accelerate your cloning workflows with reagents from NEB
AatII
Acc65I
AccI
AciI
AclI
AcuI
AflII
AgeI-HF®
AluI
AlwNI
ApaI
ApaLI
ApeKI
ApoI-HF®
AscI
AseI
AvaI
AvaII
AvrII
BaeGI
BaeI
BamHI
BbsI
BbvI
BclI-HF
BcoDI
BfuAI
BglI
BglII
BmgBI
BmtI-HF®
BpmI
BpuEI
BsaHI
BsaI-HF®v2
BsaWI
BsaXI
BseRI
BsgI
BsiWI
BsiWI-HF®
BslI
BsmAI
BsmI
BsoBI
Bsp1286I
BspCNI
BspHI
BspQI
BsrBI
BsrDI
BsrI
BssHII
BstNI
BstUI
BstXI
BstYI
BstZ17I-HF®
Bsu36I
BtgI
BtsCI
Cac8I
ClaI
CviQI
DdeI
DpnI
DpnII
DraI
DraIII-HF®
DrdI
EagI-HF®
EcoNI
EcoO109I
EcoP15I
EcoRI
EcoRV
EcoRV-HF®
Esp3I
Fnu4HI
FseI
HaeIII
HincII
HindIII-HF®
HinfI
HpaII
HphI
HpyAV
HpyCH4IV
HpyCH4V
Instant Sticky-end Ligase Master Mix
MluCI
MlyI
MnlI
MseI
MslI
MspA1I
MspI
NciI
NcoI
NcoI-HF®
NdeI
NEB® Turbo Competent E. coli (High Efficiency)
NgoMIV
NheI-HF®
NlaIII
NotI-HF®
NsiI
NspI
PacI
PaeR7I
PflMI
PshAI
PstI
PstI-HF®
PvuI-HF®
PvuII
PvuII-HF®
Q5® High-Fidelity 2X Master Mix
Q5® High-Fidelity DNA Polymerase
Q5® High-Fidelity PCR Kit
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Hot Start High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
Quick Blunting™ and Quick Ligation™ Kits
Quick Blunting™ Kit
Quick CIP
Quick Ligation™ Kit
RsaI
SacI-HF®
SacII
SalI
SbfI-HF®
ScaI-HF®
SfoI
SmaI
SpeI-HF®
SphI-HF®
SspI-HF®
StuI
StyD4I
StyI-HF®
SwaI
TaqI-v2
TfiI
TseI
TspMI
TspRI
Tth111I
XbaI
XmaI
High-throughput cloning and automation solutions
96-Well Microtiter Plate Magnetic Separation Rack
Amylose Magnetic Beads
Anti-MBP Magnetic Beads
BbsI-HF®
BsmBI-v2
BspQI
BtgZI
Chitin Magnetic Beads
dam–/dcm– Competent E. coli
Esp3I
KLD Enzyme Mix
NEB® 10-beta Competent E. coli (High Efficiency)
NEB® 5-alpha Competent E. coli (High Efficiency)
NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)
NEB® Stable Competent E. coli (High Efficiency)
NEB® Turbo Competent E. coli (High Efficiency)
NEBExpress® Cell-free E. coli Protein Synthesis System
NEBExpress® E. coli Lysis Reagent
NEBExpress® Ni-NTA Magnetic Beads
NEBridge® Ligase Master Mix
NEBuilder® HiFi DNA Assembly Master Mix
PaqCI®
Protein A Magnetic Beads
Protein G Magnetic Beads
PURExpress® In Vitro Protein Synthesis Kit
Q5® Hot Start High-Fidelity 2X Master Mix
SapI
Nucleic Acid Purification
Monarch® DNA Cleanup Binding Buffer
Monarch® DNA Cleanup Columns (5 μg)
Monarch® DNA Elution Buffer
Monarch® DNA Gel Extraction Kit
Monarch® DNA Wash Buffer
Monarch® gDNA Binding Buffer
Monarch® gDNA Blood Lysis Buffer
Monarch® gDNA Cell Lysis Buffer
Monarch® gDNA Elution Buffer
Monarch® gDNA Purification Columns
Monarch® gDNA Tissue Lysis Buffer
Monarch® gDNA Wash Buffer
Monarch® Gel Dissolving Buffer
Monarch® Genomic DNA Purification Kit
Monarch® PCR & DNA Cleanup Kit (5 μg)
Monarch® Plasmid Lysis Buffer (B2)
Monarch® Plasmid Miniprep Columns
Monarch® Plasmid Miniprep Kit
Monarch® Plasmid Neutralization Buffer (B3)
Monarch® Plasmid Resuspension Buffer (B1)
Monarch® Plasmid Wash Buffer 1
Monarch® Plasmid Wash Buffer 2
Monarch® RNase A
β-Agarase I
Site Directed Mutagenesis
DpnI
KLD Enzyme Mix
NEB® 10-beta Competent E. coli (High Efficiency)
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Site-Directed Mutagenesis Kit
Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)
SOC Outgrowth Medium
Transformation
dam–/dcm– Competent E. coli
NEB Cloning Competent E. coli Sampler
NEB® 10-beta Competent E. coli (High Efficiency)
NEB® 10-beta Electrocompetent E. coli
NEB® 5-alpha Competent E. coli (High Efficiency)
NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)
NEB® Stable Competent E. coli (High Efficiency)
NEB® Turbo Competent E. coli (High Efficiency)
SOC Outgrowth Medium
USER® Cloning
dUTP Solution
NEB® 10-beta Competent E. coli (High Efficiency)
NEB® 5-alpha Competent E. coli (High Efficiency)
NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)
NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Q5U® Hot Start High-Fidelity DNA Polymerase
Thermolabile USER® II Enzyme
USER® Enzyme
DNA Analysis
1 kb DNA Ladder
1 kb Plus DNA Ladder
1 kb Plus DNA Ladder for Safe Stains
100 bp DNA Ladder
50 bp DNA Ladder
BsmBI-v2
Fast DNA Ladder
Lambda PFG Ladder
Low Molecular Weight DNA Ladder
MidRange PFG Marker
pBR322 DNA-BstNI Digest
pBR322 DNA-MspI Digest
PCR Marker
PsiI-v2
Quick-Load® 1 kb DNA Ladder
Quick-Load® 1 kb Extend DNA Ladder
Quick-Load® 1 kb Plus DNA Ladder
Quick-Load® 100 bp DNA Ladder
Quick-Load® Purple 1 kb DNA Ladder
Quick-Load® Purple 1 kb Plus DNA Ladder
Quick-Load® Purple 100 bp DNA Ladder
Quick-Load® Purple 50 bp DNA Ladder
Quick-Load® Purple Low Molecular Weight DNA Ladder
Supercoiled DNA Ladder
TriDye™ 1 kb DNA Ladder
TriDye™ 1 kb Plus DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ Ultra Low Range DNA Ladder
λ DNA-BstEII Digest
λ DNA-HindIII Digest
λ DNA-Mono Cut Mix
ΦX174 DNA-HaeIII Digest
Colony PCR
Hot Start Taq DNA Polymerase
LongAmp® Taq DNA Polymerase
Standard Taq (Mg-free) Reaction Buffer Pack
Standard Taq Reaction Buffer Pack
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
Taq DNA Polymerase with Standard Taq Buffer
Taq DNA Polymerase with ThermoPol® Buffer
ThermoPol® Reaction Buffer
DNA Sequencing
5-methyl-dCTP
Acyclonucleotide Set
Deoxynucleotide (dNTP) Solution Mix
Deoxynucleotide (dNTP) Solution Set
DNA Polymerase I, Large (Klenow) Fragment
Klenow Fragment (3'→5' exo-)
NEBNext® dA-Tailing Module
NEBNext® End Repair Module
NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™
NEBNext® Fast DNA Library Prep Set for Ion Torrent™
Restriction Enzyme Digestion
AleI-v2
BsaI-HF®v2
BsiWI-HF®
BsmBI-v2
BstZ17I-HF®
Diluent A (with rAlbumin)
Diluent B (with rAlbumin)
Diluent C (with rAlbumin)
PsiI-v2
Recombinant Albumin, Molecular Biology Grade
DNA Assembly and Cloning
NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2)
NEBridge® Golden Gate Assembly Kit (BsmBI-v2)
Thermolabile USER® II Enzyme
Thermostable USER® III Enzyme
USER® Enzyme
NEBuilder® HiFi DNA Assembly
NEBuilder® HiFi DNA Assembly Bundle for Large Fragments
NEBuilder® HiFi DNA Assembly Cloning Kit
NEBuilder® HiFi DNA Assembly Master Mix
NEBridge® Golden Gate Assembly
BbsI
BbsI-HF®
BsaI-HF®v2
BsmBI-v2
BspQI
Esp3I
Hi-T4™ DNA Ligase
NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2)
NEBridge® Golden Gate Assembly Kit (BsmBI-v2)
NEBridge® Ligase Master Mix
PaqCI®
PluTI
Salt-T4® DNA Ligase
SapI
T4 DNA Ligase
T7 DNA Ligase
Gibson Assembly®
Gibson Assembly® Cloning Kit
Gibson Assembly® Master Mix
Phusion® High-Fidelity DNA Polymerase
Q5® High-Fidelity DNA Polymerase
T5 Exonuclease
Taq DNA Ligase
BioBrick® Assembly
EcoRI-HF®
Hi-T4™ DNA Ligase
PstI
Salt-T4® DNA Ligase
SpeI-HF®
T4 DNA Ligase
XbaI
DNA End Modification
5-hydroxymethyluridine DNA Kinase
Dephosphorylation
Antarctic Phosphatase
Antarctic Phosphatase Reaction Buffer
Quick CIP
Shrimp Alkaline Phosphatase (rSAP)
Phosphorylation (Kinase)
5-hydroxymethyluridine DNA Kinase
Quick Blunting™ and Quick Ligation™ Kits
Quick Blunting™ Kit
T4 DNA Ligase Reaction Buffer
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (3' phosphatase minus)
T4 Polynucleotide Kinase Reaction Buffer
A-tailing
Deoxynucleotide (dNTP) Solution Set
Klenow Fragment (3'→5' exo-)
NEBNext® dA-Tailing Module
NEBNext® dA-Tailing Reaction Buffer
Standard Taq (Mg-free) Reaction Buffer Pack
Standard Taq Reaction Buffer Pack
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
Taq DNA Polymerase with Standard Taq Buffer
Taq DNA Polymerase with ThermoPol® Buffer
Terminal Transferase
ThermoPol® Reaction Buffer
Blunting
DNA Polymerase I, Large (Klenow) Fragment
Mung Bean Nuclease
Quick Blunting™ and Quick Ligation™ Kits
Quick Blunting™ Kit
T4 DNA Polymerase
DNA Ligation
HiFi Taq DNA Ligase
Immobilized T4 DNA Ligase
Non-Cloning Ligation
E. coli DNA Ligase
HiFi Taq DNA Ligase
Immobilized T4 DNA Ligase
NEBNext® Quick Ligation Module
Thermostable 5´ App DNA/RNA Ligase
Cloning Ligation
9°N™ DNA Ligase
Blunt/TA Ligase Master Mix
dGTP Solution
ElectroLigase®
Hi-T4™ DNA Ligase
Immobilized T4 DNA Ligase
Instant Sticky-end Ligase Master Mix
NEBuffer™ Set (EcoRI/SspI, DpnII)
Quick Blunting™ and Quick Ligation™ Kits
Quick Ligation™ Kit
Salt-T4® DNA Ligase
StickTogether™ DNA Ligase Buffer
T3 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase Reaction Buffer
T7 DNA Ligase
Taq DNA Ligase
Taq DNA Ligase Reaction Buffer
DNA Preparation
AleI-v2
BsmBI-v2
PsiI-v2
Reverse Transcription (cDNA Synthesis)
AMV Reverse Transcriptase
Induro® Reverse Transcriptase
LunaScript® RT Master Mix Kit (Primer-free)
LunaScript® RT SuperMix
LunaScript® RT SuperMix Kit
M-MuLV Reverse Transcriptase
ProtoScript® First Strand cDNA Synthesis Kit
ProtoScript® II First Strand cDNA Synthesis Kit
ProtoScript® II Reverse Transcriptase
Template Switching RT Enzyme Mix
WarmStart® RTx Reverse Transcriptase
Restriction Enzyme Digestion
AatII
Acc65I
AccI
AciI
AclI
AcuI
AfeI
AflII
AflIII
AgeI-HF®
AhdI
AleI-v2
AluI
AlwI
AlwNI
ApaI
ApaLI
ApeKI
ApoI-HF®
AscI
AseI
AsiSI
AvaI
AvaII
AvrII
BaeGI
BaeI
BamHI
BamHI-HF®
BanI
BanII
BbsI
BbsI-HF®
BbvCI
BbvI
BccI
BceAI
BcgI
BciVI
BclI
BclI-HF
BcoDI
BfaI
BfuAI
BglI
BglII
BlpI
BmgBI
BmrI
BmtI-HF®
BpmI
Bpu10I
BpuEI
BsaAI
BsaBI
BsaHI
BsaI-HF®v2
BsaJI
BsaWI
BsaXI
BseRI
BseYI
BsgI
BsiEI
BsiHKAI
BsiWI
BslI
BsmAI
BsmBI-v2
BsmFI
BsmI
BsoBI
Bsp1286I
BspCNI
BspDI
BspEI
BspHI
BspMI
BspQI
BsrBI
BsrDI
BsrFI-v2
BsrI
BssHII
BssSI-v2
BstAPI
BstBI
BstEII-HF®
BstNI
BstUI
BstYI
Bsu36I
BtgI
BtgZI
BtsCI
BtsIMutI
BtsI-v2
Cac8I
ClaI
CspCI
CviKI-1
CviQI
DdeI
DpnI
DpnII
DraI
DraIII-HF®
DrdI
EaeI
EagI-HF®
EarI
EciI
Eco53kI
EcoNI
EcoO109I
EcoP15I
EcoRI
EcoRI-HF®
EcoRV
EcoRV-HF®
FatI
FauI
Fnu4HI
FokI
FseI
FspI
HaeII
HaeIII
HgaI
HhaI
HincII
HindIII
HindIII-HF®
HinfI
HinP1I
HpaI
HpaII
HphI
Hpy166II
Hpy188I
Hpy188III
Hpy99I
HpyCH4III
HpyCH4IV
HpyCH4V
I-CeuI
I-SceI
KasI
KpnI-HF®
MboI
MboII
MfeI-HF®
MluCI
MluI-HF®
MlyI
MmeI
MnlI
MscI
MseI
MslI
MspA1I
MspI
MspJI
MwoI
NaeI
NarI
NciI
NcoI
NcoI-HF®
NdeI
NEBuffer™ 1
NEBuffer™ 2
NEBuffer™ 3
NEBuffer™ 4
NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)
NgoMIV
NheI-HF®
NlaIII
NlaIV
NmeAIII
NotI
NotI-HF®
NruI-HF®
NsiI
NsiI-HF®
NspI
PacI
PaeR7I
PciI
PflFI
PflMI
PI-PspI
PI-SceI
PleI
PmeI
PmlI
PpuMI
PshAI
PspGI
PspOMI
PspXI
PstI-HF®
PvuI-HF®
PvuII
PvuII-HF®
rCutSmart™ Buffer
Recombinant Albumin, Molecular Biology Grade
RsaI
RsrII
SacI-HF®
SacII
S-adenosylmethionine (SAM)
SalI
SalI-HF®
SapI
Sau3AI
Sau96I
SbfI-HF®
ScaI-HF®
ScrFI
SexAI
SfaNI
SfcI
SfiI
SfoI
SgrAI
SmaI
SmlI
SnaBI
SpeI-HF®
SphI
SphI-HF®
SrfI
SspI-HF®
StuI
StyD4I
StyI-HF®
SwaI
TaqI-v2
TfiI
TseI
Tsp45I
TspMI
TspRI
Tth111I
XbaI
XcmI
XhoI
XmaI
XmnI
ZraI
PCR
3´-Biotin-GTP
3´-Desthiobiotin-GTP
5-methyl-dCTP
7-deaza-dGTP
Acyclonucleotide Set
Adenosine 5'-Triphosphate (ATP)
AMV Reverse Transcriptase
Bsu DNA Polymerase, Large Fragment
dATP Solution
Deep Vent® (exo-) DNA Polymerase
Deep Vent® DNA Polymerase
Deoxynucleotide (dNTP) Solution Mix
Deoxynucleotide (dNTP) Solution Set
DNA Polymerase I (E. coli)
DNA Polymerase I, Large (Klenow) Fragment
dUTP Solution
E. coli Poly(A) Polymerase
EpiMark® Hot Start Taq DNA Polymerase
Hemo KlenTaq®
Hot Start Taq 2X Master Mix
Hot Start Taq DNA Polymerase
Isothermal Amplification Buffer II Pack
Isothermal Amplification Buffer Pack
Klenow Fragment (3'→5' exo-)
LongAmp® Hot Start Taq 2X Master Mix
LongAmp® Hot Start Taq DNA Polymerase
LongAmp® Taq 2X Master Mix
LongAmp® Taq DNA Polymerase
LongAmp® Taq PCR Kit
M13KO7 Helper Phage
Magnesium Chloride (MgCl2) Solution
Magnesium Sulfate (MgSO4) Solution
M-MuLV Reverse Transcriptase
Multiplex PCR 5X Master Mix
NEBNext® High-Fidelity 2X PCR Master Mix
NEBNext® Q5® Hot Start HiFi PCR Master Mix
NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module
NEBNext® Ultra™ II Q5® Master Mix
Oligo d(T)18 mRNA Primer
Oligo d(T)23 VN
OneTaq® 2X Master Mix with Standard Buffer
OneTaq® DNA Polymerase
OneTaq® Hot Start 2X Master Mix with GC Buffer
OneTaq® Hot Start 2X Master Mix with Standard Buffer
OneTaq® Hot Start DNA Polymerase
OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer
OneTaq® One-Step RT-PCR Kit
OneTaq® RT-PCR Kit
OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
Phusion® GC Buffer Pack
Phusion® HF Buffer Pack
Phusion® High-Fidelity DNA Polymerase
Phusion® High-Fidelity PCR Master Mix with GC Buffer
Phusion® High-Fidelity PCR Master Mix with HF Buffer
Phusion® Hot Start Flex 2X Master Mix
Phusion® Hot Start Flex DNA Polymerase
Phusion® High-Fidelity PCR Kit
Poly(U) Polymerase
PreCR® Repair Mix
ProtoScript® First Strand cDNA Synthesis Kit
ProtoScript® II First Strand cDNA Synthesis Kit
ProtoScript® II Reverse Transcriptase
Q5® High-Fidelity 2X Master Mix
Q5® High-Fidelity DNA Polymerase
Q5® High-Fidelity PCR Kit
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Hot Start High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
Quick Blunting™ Kit
Quick-Load® Taq 2X Master Mix
Random Primer 9
Ribonucleotide Solution Set
Ribonucleotide Solution Mix
RNase H
RNase Inhibitor, Murine
SP6 RNA Polymerase
Standard Taq (Mg-free) Reaction Buffer Pack
Standard Taq Reaction Buffer Pack
Sulfolobus DNA Polymerase IV
T3 RNA Polymerase
T4 DNA Polymerase
T4 RNA Ligase 2, truncated KQ
T7 DNA Polymerase (unmodified)
Taq 2X Master Mix
Taq 5X Master Mix
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
Taq DNA Polymerase with Standard Taq Buffer
Taq DNA Polymerase with ThermoPol® Buffer
Taq PCR Kit
Terminal Transferase
ThermoPol® Reaction Buffer
Thermostable RNase H
Vent® (exo-) DNA Polymerase
Vent® DNA Polymerase
WarmStart® RTx Reverse Transcriptase
β-Nicotinamide adenine dinucleotide (NAD+)
Fast Cloning: Accelerate your cloning workflows with reagents from NEB
AatII
Acc65I
AccI
AciI
AclI
AcuI
AflII
AgeI-HF®
AluI
AlwNI
ApaI
ApaLI
ApeKI
ApoI-HF®
AscI
AseI
AvaI
AvaII
AvrII
BaeGI
BaeI
BamHI
BbsI
BbvI
BclI-HF
BcoDI
BfuAI
BglI
BglII
BmgBI
BmtI-HF®
BpmI
BpuEI
BsaHI
BsaI-HF®v2
BsaWI
BsaXI
BseRI
BsgI
BsiWI
BsiWI-HF®
BslI
BsmAI
BsmI
BsoBI
Bsp1286I
BspCNI
BspHI
BspQI
BsrBI
BsrDI
BsrI
BssHII
BstNI
BstUI
BstXI
BstYI
BstZ17I-HF®
Bsu36I
BtgI
BtsCI
Cac8I
ClaI
CviQI
DdeI
DpnI
DpnII
DraI
DraIII-HF®
DrdI
EagI-HF®
EcoNI
EcoO109I
EcoP15I
EcoRI
EcoRV
EcoRV-HF®
Esp3I
Fnu4HI
FseI
HaeIII
HincII
HindIII-HF®
HinfI
HpaII
HphI
HpyAV
HpyCH4IV
HpyCH4V
Instant Sticky-end Ligase Master Mix
MluCI
MlyI
MnlI
MseI
MslI
MspA1I
MspI
NciI
NcoI
NcoI-HF®
NdeI
NEB® Turbo Competent E. coli (High Efficiency)
NgoMIV
NheI-HF®
NlaIII
NotI-HF®
NsiI
NspI
PacI
PaeR7I
PflMI
PshAI
PstI
PstI-HF®
PvuI-HF®
PvuII
PvuII-HF®
Q5® High-Fidelity 2X Master Mix
Q5® High-Fidelity DNA Polymerase
Q5® High-Fidelity PCR Kit
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Hot Start High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
Quick Blunting™ and Quick Ligation™ Kits
Quick Blunting™ Kit
Quick CIP
Quick Ligation™ Kit
RsaI
SacI-HF®
SacII
SalI
SbfI-HF®
ScaI-HF®
SfoI
SmaI
SpeI-HF®
SphI-HF®
SspI-HF®
StuI
StyD4I
StyI-HF®
SwaI
TaqI-v2
TfiI
TseI
TspMI
TspRI
Tth111I
XbaI
XmaI
High-throughput cloning and automation solutions
96-Well Microtiter Plate Magnetic Separation Rack
Amylose Magnetic Beads
Anti-MBP Magnetic Beads
BbsI-HF®
BsmBI-v2
BspQI
BtgZI
Chitin Magnetic Beads
dam–/dcm– Competent E. coli
Esp3I
KLD Enzyme Mix
NEB® 10-beta Competent E. coli (High Efficiency)
NEB® 5-alpha Competent E. coli (High Efficiency)
NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)
NEB® Stable Competent E. coli (High Efficiency)
NEB® Turbo Competent E. coli (High Efficiency)
NEBExpress® Cell-free E. coli Protein Synthesis System
NEBExpress® E. coli Lysis Reagent
NEBExpress® Ni-NTA Magnetic Beads
NEBridge® Ligase Master Mix
NEBuilder® HiFi DNA Assembly Master Mix
PaqCI®
Protein A Magnetic Beads
Protein G Magnetic Beads
PURExpress® In Vitro Protein Synthesis Kit
Q5® Hot Start High-Fidelity 2X Master Mix
SapI
Nucleic Acid Purification
Monarch® DNA Cleanup Binding Buffer
Monarch® DNA Cleanup Columns (5 μg)
Monarch® DNA Elution Buffer
Monarch® DNA Gel Extraction Kit
Monarch® DNA Wash Buffer
Monarch® gDNA Binding Buffer
Monarch® gDNA Blood Lysis Buffer
Monarch® gDNA Cell Lysis Buffer
Monarch® gDNA Elution Buffer
Monarch® gDNA Purification Columns
Monarch® gDNA Tissue Lysis Buffer
Monarch® gDNA Wash Buffer
Monarch® Gel Dissolving Buffer
Monarch® Genomic DNA Purification Kit
Monarch® PCR & DNA Cleanup Kit (5 μg)
Monarch® Plasmid Lysis Buffer (B2)
Monarch® Plasmid Miniprep Columns
Monarch® Plasmid Miniprep Kit
Monarch® Plasmid Neutralization Buffer (B3)
Monarch® Plasmid Resuspension Buffer (B1)
Monarch® Plasmid Wash Buffer 1
Monarch® Plasmid Wash Buffer 2
Monarch® RNase A
β-Agarase I
Site Directed Mutagenesis
DpnI
KLD Enzyme Mix
NEB® 10-beta Competent E. coli (High Efficiency)
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Site-Directed Mutagenesis Kit
Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)
SOC Outgrowth Medium
Transformation
dam–/dcm– Competent E. coli
NEB Cloning Competent E. coli Sampler
NEB® 10-beta Competent E. coli (High Efficiency)
NEB® 10-beta Electrocompetent E. coli
NEB® 5-alpha Competent E. coli (High Efficiency)
NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)
NEB® Stable Competent E. coli (High Efficiency)
NEB® Turbo Competent E. coli (High Efficiency)
SOC Outgrowth Medium
USER® Cloning
dUTP Solution
NEB® 10-beta Competent E. coli (High Efficiency)
NEB® 5-alpha Competent E. coli (High Efficiency)
Q5U® Hot Start High-Fidelity DNA Polymerase
Thermolabile USER® II Enzyme
USER® Enzyme
Applications of USER® and Thermolabile USER II Enzymes
NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)
NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Q5U® Hot Start High-Fidelity DNA Polymerase
Thermolabile USER® II Enzyme
USER® Enzyme
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