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Cloning & Synthetic Biology


Cloning & Synthetic Biology

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Order Cas9 Nuclease, S. pyogenes

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Order Endoglycoceramidase I (EGCase I)

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Order EnGen® Cas9 NLS, S. pyogenes

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Order EnGen® Spy Cas9 Nickase

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Order EnGen® Spy dCas9 (SNAP-tag®)

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Order NEB® 10-beta/Stable Outgrowth Medium

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Order NEB® PCR Cloning Kit

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Order NEB® PCR Cloning Kit (Without Competent Cells)

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Order NEBuilder® HiFi DNA Assembly Bundle for Large Fragments

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Order NEBuilder® HiFi DNA Assembly Cloning Kit

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Order NEBuilder® HiFi DNA Assembly Master Mix

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Order Nuclease-free Water

DNA Preparation

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Order AMV Reverse Transcriptase

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Order M-MuLV Reverse Transcriptase

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Order ProtoScript® First Strand cDNA Synthesis Kit

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Order ProtoScript® II First Strand cDNA Synthesis Kit

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Order ProtoScript® II Reverse Transcriptase

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Order WarmStart® RTx Reverse Transcriptase

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Order AatII

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Order AbaSI

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Order Acc65I

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Order AccI

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Order AciI

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Order AclI

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Order AcuI

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Order AfeI

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Order AflII

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Order AflIII

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Order AgeI

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Order AgeI-HF®

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Order AhdI

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Order AleI

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Order AluI

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Order AlwNI

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Order ApaI

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Order ApaLI

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Order ApeKI

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Order ApoI-HF®

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Order AscI

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Order AseI

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Order AsiSI

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Order AvaI

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Order AvaII

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Order AvrII

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Order BaeGI

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Order BaeI

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Order BamHI

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Order BamHI-HF®

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Order BanI

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Order BanII

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Order BbsI

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Order BbsI-HF®

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Order BbvCI

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Order BbvI

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Order BccI

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Order BceAI

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Order BcgI

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Order BciVI

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Order BclI

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Order BclI-HF

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Order BcoDI

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Order BfaI

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Order BfuAI

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Order BglI

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Order BglII

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Order BlpI

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Order BmgBI

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Order BmrI

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Order BmtI

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Order BmtI-HF®

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Order BpmI

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Order Bpu10I

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Order BpuEI

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Order BSA, Molecular Biology Grade

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Order BsaAI

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Order BsaBI

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Order BsaHI

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Order BsaI

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Order BsaI-HF®

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Order BsaJI

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Order BsaWI

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Order BsaXI

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Order BseRI

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Order BseYI

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Order BsgI

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Order BsiEI

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Order BsiHKAI

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Order BsiWI

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Order BslI

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Order BsmAI

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Order BsmBI

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Order BsmFI

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Order BsmI

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Order BsoBI

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Order Bsp1286I

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Order BspCNI

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Order BspDI

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Order BspEI

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Order BspHI

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Order BspMI

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Order BspQI

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Order BsrBI

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Order BsrDI

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Order BsrFαI

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Order BsrGI

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Order BsrI

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Order BssHII

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Order BssSαI

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Order BstAPI

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Order BstBI

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Order BstEII

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Order BstEII-HF®

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Order BstNI

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Order BstUI

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Order BstYI

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Order Bsu36I

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Order BtgI

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Order BtgZI

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Order BtsCI

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Order BtsIMutI

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Order BtsαI

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Order Cac8I

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Order ClaI

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Order CspCI

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Order CutSmart® Buffer

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Order CviAII

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Order CviKI-1

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Order CviQI

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Order DdeI

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Order Diluent A

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Order Diluent B

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Order Diluent C

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Order DpnI

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Order DpnII

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Order DraI

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Order DraIII-HF®

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Order DrdI

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Order EaeI

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Order EagI

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Order EagI-HF®

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Order EarI

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Order EciI

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Order Eco53kI

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Order EcoNI

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Order EcoO109I

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Order EcoP15I

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Order EcoRI

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Order EcoRI-HF®

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Order EcoRV

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Order EcoRV-HF®

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Order FatI

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Order FauI

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Order Fnu4HI

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Order FokI

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Order FseI

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Order FspEI

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Order FspI

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Order HaeII

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Order HaeIII

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Order HgaI

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Order HhaI

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Order HincII

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Order HindIII

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Order HindIII-HF®

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Order HinfI

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Order HinP1I

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Order HpaI

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Order HpaII

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Order HphI

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Order Hpy166II

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Order Hpy188III

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Order Hpy99I

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Order HpyCH4III

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Order HpyCH4IV

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Order HpyCH4V

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Order I-CeuI

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Order I-SceI

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Order KasI

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Order KpnI

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Order KpnI-HF®

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Order LpnPI

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Order MboI

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Order MboII

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Order McrBC

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Order MfeI

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Order MfeI-HF®

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Order MluCI

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Order MluI

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Order MluI-HF®

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Order MlyI

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Order MmeI

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Order MnlI

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Order MscI

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Order MseI

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Order MslI

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Order MspA1I

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Order MspI

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Order MspJI

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Order MwoI

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Order NaeI

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Order NarI

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Order NciI

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Order NcoI

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Order NcoI-HF®

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Order NdeI

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Order NEBuffer™ 1

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Order NEBuffer™ 1.1

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Order NEBuffer™ 2

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Order NEBuffer™ 2.1

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Order NEBuffer™ 3

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Order NEBuffer™ 3.1

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Order NEBuffer™ 4

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Order NEBuffer™ EcoRI

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Order NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

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Order NgoMIV

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Order NheI

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Order NheI-HF®

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Order NlaIII

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Order NlaIV

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Order NmeAIII

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Order NotI

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Order NotI-HF®

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Order NruI

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Order NruI-HF®

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Order NsiI

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Order NsiI-HF®

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Order NspI

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Order PacI

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Order PaeR7I

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Order PciI

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Order PflFI

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Order PflMI

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Order PI-PspI

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Order PI-SceI

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Order PleI

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Order PmeI

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Order PpuMI

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Order PshAI

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Order PsiI

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Order PspGI

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Order PspOMI

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Order PspXI

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Order PstI-HF®

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Order PvuI

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Order PvuI-HF®

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Order PvuII

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Order PvuII-HF®

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Order RsaI

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Order RsrII

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Order SacI

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Order SacI-HF®

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Order SacII

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Order S-adenosylmethionine (SAM)

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Order SalI

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Order SalI-HF®

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Order SapI

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Order Sau3AI

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Order Sau96I

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Order SbfI

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Order SbfI-HF®

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Order ScaI-HF®

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Order ScrFI

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Order SexAI

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Order SfaNI

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Order SfcI

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Order SfiI

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Order SfoI

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Order SgrAI

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Order SmaI

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Order SmlI

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Order SnaBI

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Order SpeI

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Order SpeI-HF®

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Order SphI

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Order SphI-HF®

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Order SrfI

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Order SspI

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Order SspI-HF®

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Order StuI

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Order StyD4I

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Order StyI

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Order StyI-HF®

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Order SwaI

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Order TaqαI

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Order TfiI

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Order TseI

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Order Tsp45I

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Order TspMI

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Order TspRI

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Order Tth111I

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Order XbaI

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Order XcmI

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Order XhoI

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Order XmaI

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Order XmnI

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Order ZraI

Nucleic Acid Purification

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Order Monarch® DNA Cleanup Binding Buffer

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Order Monarch® DNA Cleanup Columns (5 μg)

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Order Monarch® DNA Elution Buffer

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Order Monarch® DNA Gel Extraction Kit

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Order Monarch® DNA Wash Buffer

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Order Monarch® Gel Dissolving Buffer

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Order Monarch® PCR & DNA Cleanup Kit (5 μg)

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Order Monarch® Plasmid Lysis Buffer (B2)

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Order Monarch® Plasmid Miniprep Columns

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Order Monarch® Plasmid Miniprep Kit 

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Order Monarch® Plasmid Resuspension Buffer (B1)

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Order Monarch® Plasmid Wash Buffer 1

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Order Monarch® Plasmid Wash Buffer 2

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Order Monarch® Plasmid Neutralization Buffer (B3)

DNA End Modification

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Order Alkaline Phosphatase, Calf Intestinal (CIP)

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Order Antarctic Phosphatase

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Order Antarctic Phosphatase Reaction Buffer

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Order Quick Dephosphorylation Kit

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Order Shrimp Alkaline Phosphatase (rSAP)

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Order Quick Blunting™ and Quick Ligation™ Kits

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Order Quick Blunting™ Kit

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Order T4 DNA Ligase Reaction Buffer

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Order T4 Polynucleotide Kinase

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Order T4 Polynucleotide Kinase (3' phosphatase minus)

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Order T4 Polynucleotide Kinase Reaction Buffer

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Order Deoxynucleotide (dNTP) Solution Set

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Order Klenow Fragment (3'→5' exo-)

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Order NEBNext® dA-Tailing Module

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Order NEBNext® dA-Tailing Reaction Buffer

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Order Standard Taq (Mg-free) Reaction Buffer Pack

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Order Standard Taq Reaction Buffer Pack

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Order Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

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Order Taq DNA Polymerase with Standard Taq Buffer

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Order Taq DNA Polymerase with ThermoPol® Buffer

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Order Terminal Transferase

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Order ThermoPol® II (Mg-free) Reaction Buffer Pack

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Order ThermoPol® Reaction Buffer Pack

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Order DNA Polymerase I, Large (Klenow) Fragment

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Order Mung Bean Nuclease

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Order Quick Blunting™ and Quick Ligation™ Kits

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Order Quick Blunting™ Kit

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Order T4 DNA Polymerase

DNA Assembly and Cloning

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Order NEB® Golden Gate Assembly Mix

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Order Thermolabile USER® II Enzyme

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Order USER® Enzyme

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Order NEBuilder® HiFi DNA Assembly Bundle for Large Fragments

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Order NEBuilder® HiFi DNA Assembly Cloning Kit

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Order NEBuilder® HiFi DNA Assembly Master Mix

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Order Gibson Assembly® Cloning Kit

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Order Gibson Assembly® Master Mix

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Order Phusion® High-Fidelity DNA Polymerase

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Order Q5® High-Fidelity DNA Polymerase

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Order T5 Exonuclease

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Order Taq DNA Ligase

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Order BioBrick® Assembly Kit

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Order EcoRI-HF®

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Order NEBuffer™ 2.1

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Order PstI

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Order SpeI

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Order SpeI-HF®

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Order T4 DNA Ligase

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Order XbaI

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Order BbsI

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Order BbsI-HF®

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Order BsaI

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Order BsaI-HF®

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Order BsmBI

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Order Exonuclease V (RecBCD)

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Order NEB® Golden Gate Assembly Mix

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Order Quick Ligation™ Kit

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Order T4 DNA Ligase

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Order T7 DNA Ligase

DNA Ligation

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Order HiFi Taq DNA Ligase

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Order E. coli DNA Ligase

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Order HiFi Taq DNA Ligase

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Order NEBNext® Quick Ligation Module

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Order Thermostable 5´ AppDNA/RNA Ligase

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Order 9°N™ DNA Ligase

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Order Blunt/TA Ligase Master Mix

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Order ElectroLigase®

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Order Instant Sticky-end Ligase Master Mix

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Order Quick Blunting™ and Quick Ligation™ Kits

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Order Quick Ligation™ Kit

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Order T3 DNA Ligase

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Order T4 DNA Ligase

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Order T4 DNA Ligase Reaction Buffer

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Order T7 DNA Ligase

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Order Taq DNA Ligase

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Order Taq DNA Ligase Reaction Buffer

USER® Cloning

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Order NEB® 10-beta Competent E. coli (High Efficiency)

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Order NEB® 5-alpha Competent E. coli (High Efficiency)

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Order Thermolabile USER® II Enzyme

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Order USER® Enzyme

Transformation

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Order dam-/dcm- Competent E. coli

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Order NEB® 10-beta Competent E. coli (High Efficiency)

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Order NEB® 10-beta Electrocompetent E. coli

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Order NEB® 5-alpha Competent E. coli (High Efficiency)

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Order NEB® 5-alpha Competent E. coli (Subcloning Efficiency)

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Order NEB® 5-alpha Electrocompetent E. coli

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Order NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

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Order NEB® Stable Competent E. coli (High Efficiency)

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Order NEB® Turbo Competent E. coli (High Efficiency)

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Order NEB® Turbo Electrocompetent E. coli

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Order SOC Outgrowth Medium

DNA Isolation

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Order β-Agarase I

DNA Analysis

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Order 1 kb DNA Ladder

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Order 100 bp DNA Ladder

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Order 2-Log DNA Ladder (0.1-10.0 kb)

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Order 50 bp DNA Ladder

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Order Fast DNA Ladder

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Order Lambda PFG Ladder

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Order Low Molecular Weight DNA Ladder

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Order MidRange PFG Marker

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Order pBR322 DNA-BstNI Digest

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Order pBR322 DNA-MspI Digest

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Order PCR Marker

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Order Quick-Load® 1 kb DNA Ladder

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Order Quick-Load® 1 kb Extend DNA Ladder

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Order Quick-Load® 100 bp DNA Ladder

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Order Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)

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Order Quick-Load® Purple 1 kb DNA Ladder

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Order Quick-Load® Purple 100 bp DNA Ladder

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Order Quick-Load® Purple 2-Log DNA Ladder (0.1 - 10.0 kb)

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Order Quick-Load® Purple 50 bp DNA Ladder

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Order Quick-Load® Purple Low Molecular Weight DNA Ladder

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Order Supercoiled DNA Ladder

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Order TriDye™ 1 kb DNA Ladder

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Order TriDye™ 100 bp DNA Ladder

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Order TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

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Order λ DNA-BstEII Digest

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Order λ DNA-HindIII Digest

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Order λ DNA-Mono Cut Mix

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Order ΦX174 DNA-Hae III Digest

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Order BsaI-HF®

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Order BsiWI-HF®

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Order BstZ17I-HF®

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Order Hot Start Taq DNA Polymerase

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Order LongAmp® Taq DNA Polymerase

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Order Standard Taq (Mg-free) Reaction Buffer Pack

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Order Standard Taq Reaction Buffer Pack

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Order Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

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Order Taq DNA Polymerase with Standard Taq Buffer

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Order Taq DNA Polymerase with ThermoPol® Buffer

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Order ThermoPol® II (Mg-free) Reaction Buffer Pack

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Order ThermoPol® Reaction Buffer Pack

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Order 5-methyl-dCTP

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Order Acyclonucleotide Set

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Order Deoxynucleotide (dNTP) Solution Mix

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Order Deoxynucleotide (dNTP) Solution Set

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Order DNA Polymerase I, Large (Klenow) Fragment

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Order Klenow Fragment (3'→5' exo-)

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Order NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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Order NEBNext® dA-Tailing Module

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Order NEBNext® End Repair Module

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Order NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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Order NEBNext® Fast DNA Library Prep Set for Ion Torrent™

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Order NEBNext® DNA Library Prep Master Mix Set for 454™

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Order NEBNext® DNA Library Prep Master Mix Set for Illumina®

Site Directed Mutagenesis

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Order DpnI

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Order KLD Enzyme Mix

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Order NEB® 10-beta Competent E. coli (High Efficiency)

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Order Q5® Hot Start High-Fidelity 2X Master Mix

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Order Q5® Site-Directed Mutagenesis Kit

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Order Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)

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Order SOC Outgrowth Medium

DNA Preparation

Reverse Transcription (cDNA Synthesis)

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Order AMV Reverse Transcriptase

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Order M-MuLV Reverse Transcriptase

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Order ProtoScript® First Strand cDNA Synthesis Kit

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Order ProtoScript® II First Strand cDNA Synthesis Kit

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Order ProtoScript® II Reverse Transcriptase

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Order WarmStart® RTx Reverse Transcriptase

Restriction Enzyme Digestion

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Order AatII

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Order AbaSI

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Order Acc65I

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Order AccI

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Order AciI

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Order AclI

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Order AcuI

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Order AfeI

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Order AflII

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Order AflIII

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Order AgeI

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Order AgeI-HF®

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Order AhdI

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Order AleI

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Order AluI

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Order AlwNI

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Order ApaI

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Order ApaLI

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Order ApeKI

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Order ApoI-HF®

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Order AscI

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Order AseI

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Order AsiSI

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Order AvaI

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Order AvaII

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Order AvrII

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Order BaeGI

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Order BaeI

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Order BamHI

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Order BamHI-HF®

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Order BanI

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Order BanII

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Order BbsI

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Order BbsI-HF®

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Order BbvCI

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Order BbvI

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Order BccI

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Order BceAI

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Order BcgI

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Order BciVI

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Order BclI

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Order BclI-HF

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Order BcoDI

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Order BfaI

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Order BfuAI

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Order BglI

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Order BglII

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Order BlpI

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Order BmgBI

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Order BmrI

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Order BmtI

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Order BmtI-HF®

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Order BpmI

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Order Bpu10I

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Order BpuEI

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Order BSA, Molecular Biology Grade

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Order BsaAI

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Order BsaBI

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Order BsaHI

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Order BsaI

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Order BsaI-HF®

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Order BsaJI

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Order BsaWI

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Order BsaXI

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Order BseRI

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Order BseYI

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Order BsgI

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Order BsiEI

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Order BsiHKAI

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Order BsiWI

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Order BslI

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Order BsmAI

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Order BsmBI

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Order BsmFI

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Order BsmI

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Order BsoBI

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Order Bsp1286I

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Order BspCNI

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Order BspDI

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Order BspEI

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Order BspHI

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Order BspMI

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Order BspQI

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Order BsrBI

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Order BsrDI

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Order BsrFαI

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Order BsrGI

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Order BsrI

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Order BssHII

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Order BssSαI

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Order BstAPI

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Order BstBI

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Order BstEII

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Order BstEII-HF®

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Order BstNI

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Order BstUI

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Order BstYI

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Order Bsu36I

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Order BtgI

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Order BtgZI

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Order BtsCI

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Order BtsIMutI

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Order BtsαI

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Order Cac8I

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Order ClaI

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Order CspCI

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Order CutSmart® Buffer

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Order CviAII

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Order CviKI-1

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Order CviQI

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Order DdeI

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Order Diluent A

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Order Diluent B

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Order Diluent C

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Order DpnI

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Order DpnII

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Order DraI

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Order DraIII-HF®

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Order DrdI

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Order EaeI

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Order EagI

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Order EagI-HF®

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Order EarI

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Order EciI

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Order Eco53kI

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Order EcoNI

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Order EcoO109I

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Order EcoP15I

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Order EcoRI

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Order EcoRI-HF®

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Order EcoRV

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Order EcoRV-HF®

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Order FatI

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Order FauI

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Order Fnu4HI

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Order FokI

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Order FseI

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Order FspEI

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Order FspI

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Order HaeII

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Order HaeIII

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Order HgaI

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Order HhaI

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Order HincII

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Order HindIII

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Order HindIII-HF®

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Order HinfI

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Order HinP1I

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Order HpaI

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Order HpaII

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Order HphI

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Order Hpy166II

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Order Hpy188III

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Order Hpy99I

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Order HpyCH4III

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Order HpyCH4IV

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Order HpyCH4V

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Order I-CeuI

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Order I-SceI

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Order KasI

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Order KpnI

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Order KpnI-HF®

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Order LpnPI

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Order MboI

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Order MboII

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Order McrBC

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Order MfeI

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Order MfeI-HF®

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Order MluCI

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Order MluI

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Order MluI-HF®

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Order MlyI

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Order MmeI

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Order MnlI

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Order MscI

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Order MseI

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Order MslI

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Order MspA1I

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Order MspI

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Order MspJI

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Order MwoI

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Order NaeI

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Order NarI

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Order NciI

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Order NcoI

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Order NcoI-HF®

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Order NdeI

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Order NEBuffer™ 1

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Order NEBuffer™ 1.1

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Order NEBuffer™ 2

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Order NEBuffer™ 2.1

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Order NEBuffer™ 3

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Order NEBuffer™ 3.1

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Order NEBuffer™ 4

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Order NEBuffer™ EcoRI

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Order NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

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Order NgoMIV

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Order NheI

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Order NheI-HF®

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Order NlaIII

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Order NlaIV

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Order NmeAIII

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Order NotI

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Order NotI-HF®

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Order NruI

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Order NruI-HF®

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Order NsiI

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Order NsiI-HF®

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Order NspI

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Order PacI

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Order PaeR7I

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Order PciI

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Order PflFI

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Order PflMI

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Order PI-PspI

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Order PI-SceI

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Order PleI

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Order PmeI

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Order PpuMI

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Order PshAI

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Order PsiI

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Order PspGI

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Order PspOMI

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Order PspXI

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Order PstI-HF®

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Order PvuI

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Order PvuI-HF®

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Order PvuII

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Order PvuII-HF®

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Order RsaI

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Order RsrII

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Order SacI

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Order SacI-HF®

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Order SacII

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Order S-adenosylmethionine (SAM)

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Order SalI

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Order SalI-HF®

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Order SapI

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Order Sau3AI

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Order Sau96I

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Order SbfI

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Order SbfI-HF®

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Order ScaI-HF®

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Order ScrFI

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Order SexAI

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Order SfaNI

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Order SfcI

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Order SfiI

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Order SfoI

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Order SgrAI

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Order SmaI

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Order SmlI

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Order SnaBI

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Order SpeI

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Order SpeI-HF®

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Order SphI

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Order SphI-HF®

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Order SrfI

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Order SspI

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Order SspI-HF®

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Order StuI

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Order StyD4I

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Order StyI

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Order StyI-HF®

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Order SwaI

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Order TaqαI

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Order TfiI

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PCR

Nucleic Acid Purification

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DNA End Modification

Dephosphorylation

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Phosphorylation (Kinase)

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A-tailing

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Blunting

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DNA Assembly and Cloning

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NEBuilder® HiFi DNA Assembly

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Gibson Assembly®

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BioBrick® Assembly

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Golden Gate Assembly

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DNA Ligation

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Non-Cloning Ligation

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Cloning Ligation

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USER® Cloning

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Order Thermolabile USER® II Enzyme

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Transformation

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DNA Isolation

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DNA Analysis

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Order Quick-Load® Purple 1 kb DNA Ladder

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Order Quick-Load® Purple Low Molecular Weight DNA Ladder

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Order Supercoiled DNA Ladder

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Order TriDye™ 1 kb DNA Ladder

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Order TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

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Order λ DNA-BstEII Digest

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Order λ DNA-Mono Cut Mix

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Restriction Enzyme Digestion

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Colony PCR

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Order Standard Taq (Mg-free) Reaction Buffer Pack

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Order Taq DNA Polymerase with Standard Taq Buffer

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Order Taq DNA Polymerase with ThermoPol® Buffer

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Order ThermoPol® II (Mg-free) Reaction Buffer Pack

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Order ThermoPol® Reaction Buffer Pack

DNA Sequencing

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Order 5-methyl-dCTP

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Order Acyclonucleotide Set

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Order Deoxynucleotide (dNTP) Solution Mix

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Order DNA Polymerase I, Large (Klenow) Fragment

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Order Klenow Fragment (3'→5' exo-)

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Order NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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Order NEBNext® Fast DNA Library Prep Set for Ion Torrent™

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Order NEBNext® DNA Library Prep Master Mix Set for 454™

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Order NEBNext® DNA Library Prep Master Mix Set for Illumina®

Site Directed Mutagenesis

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Order DpnI

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Order NEB® 10-beta Competent E. coli (High Efficiency)

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Order Q5® Hot Start High-Fidelity 2X Master Mix

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Order Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)

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Order SOC Outgrowth Medium

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