Genome Editing Glossary

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    Like any new technique, genome editing with CRISPR/Cas9 comes with a list of acronyms and abbreviations. This list should help you to familiarize yourself with the lingo and language of CRISPR/Cas9 studies.
    Cas= CRISPR-associated genes
    Cas9, Csn1 = a CRISPR-associated protein containing two nuclease domains, that is programmed by small RNAs to cleave DNA
    crDNA = CRISPR RNA
    dCAS9 = nuclease-deficent Cas9
    DSB = Double-Stranded Break
    gRNA = guide RNA
    HDR = Homology-Directed Repair
    HNH = an endonuclease domain named for characteristic histidine and asparagine residues
    Indel = insertion and/or deletion
    NHEJ = Non-Homologous End Joining
    PAM = Protospacer-Adjacent Motif
    RuvC = an endonuclease domain named for an E. coli protein involved in DNA repair
    sgRNA = single guide RNA
    tracrRNA, trRNA = trans-activating crRNA
    TALEN = Transcription-Activator Like Effector Nuclease
    ZFN = Zinc-Finger Nuclease