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Fast Cloning: Accelerate your cloning workflows with reagents from NEB

Fast Cloning: Accelerate your cloning workflows with reagents from NEB

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Tools & Resources

Other Tools and Resources

ONLINE TOOLS

NEBcloner^®

For products, protocols and tips for each step of your traditional cloning experiment

NEBioCalculator^®

For help with scientific calculations and conversions for DNA and RNA

NEBcutter^®

To identify the restriction sites within your DNA sequence

BROCHURES & TECHNICAL GUIDES

Molecular Cloning Technical Guide

Get help with product selection, protocols, tips for optimization and troubleshooting.

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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Videos

Overview of Traditional Cloning

Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly into vectors and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more in this tutorial about the benefits and disadvantages of Traditional Cloning.
NEBUILDER^® HiFi: the next generation of DNA Assembly

In this webinar, we will explore some of the latest applications for NEBuilder HiFi DNA Assembly. View full Q & A summary.
Golden Gate Assembly Workflow

Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments.
Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly^® or Gibson Assembly^®

Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit.
TIME-SAVER™ Qualified Restriction Enzymes

How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.
DNA Blunting Tutorial

The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how.
Cloning With Restriction Enzymes

Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.
Tips for Successful Transformations with NEB^® Competent Cells

Follow these tips to get superior results.
Why Choose Q5^® High-fidelity Polymerase?

Not sure why Q5® is your best choice for high-fidelity amplification of GC-rich targets? NEB's scientists will show you why we call Q5 an "ultra-high fidelity polymerase".
Monarch^® PCR & DNA Cleanup Kit Protocol

Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).
Fast transformation plating with glass beads

In this video, see how transformation plating can be fast and easy using glass beads.

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