Fast Cloning: Accelerate your cloning workflows with reagents from NEB

While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. Race to NEB for the highest quality products that save valuable time!

Through May 31, 2018: Ask for your free Fast Sample Pack which includes a sample of Instant Sticky-end Ligase Master Mix and Quick Dephosphorylation Kit.










Other Tools and Resources



For products, protocols and tips for each step of your traditional cloning experiment


For help with scientific calculations and conversions for DNA and RNA


To identify the restriction sites within your DNA sequence



Molecular Cloning Technical Guide

Get help with product selection, protocols, tips for optimization and troubleshooting.

Reagents & Tools for Molecular Cloning Brochure

Learn about NEB's recommended products for cloning.


Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. OverviewOfTraditionalCloning_720

    Overview of Traditional Cloning

    Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more about the benefits and disadvantages of Traditional Cloning.

  2. NEBTVwebinar_NEBuilderHiFi_720

    NEBuilder HiFi: the next generation of DNA Assembly

    In this webinar, we will explore some of the latest applications for NEBuilder HiFi DNA Assembly. View full Q & A summary.

  3. GoldenGateAssemblyWorkflow_720

    Golden Gate Assembly Workflow

    Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments.

  4. PrimerDesignFragmentAssembly_720

    Primer Design and Fragment Assembly Using NEBuilder® HiFi DNA Assembly or Gibson Assembly® E5510 E5520

    Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit.

  5. TimeSaverREs_video_thumb

    Time-Saver Qualified Restriction Enzymes

    How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.

  6. DNABluntingVideo_thumb

    DNA Blunting Tutorial

    The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how.

  7. CloningWithREsVideo_thumb

    Cloning With Restriction Enzymes

    Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.

  8. TransformationTips_VideoThumb_282x210_0616

    Tips for Successful Transformations with NEB Competent Cells

    Follow these tips to get superior results.

  9. WhichLigase_thumb

    How do I choose the best DNA Ligase?

    NEB continues to develop and produce the most extensive commercially available selection of high-quality, and performance-optimized DNA ligases and ligase master mixes for your ligation needs.

  10. Why_Choose_Q5_TN

    Why Choose Q5 High-fidelity Polymerase?

    Not sure why Q5® is your best choice for high-fidelity amplification of GC-rich targets? NEB's scientists will show you why we call Q5 an "ultra-high fidelity polymerase".

  11. Monarch_PCRCleanupProtocol_VideoThumb

    Monarch PCR & DNA Cleanup Kit protocol

    Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).