While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. Race to NEB for the highest quality products that save valuable time!
Other Tools and Resources
For products, protocols and tips for each step of your traditional cloning experiment
For help with scientific calculations and conversions for DNA and RNA
To identify the restriction sites within your DNA sequence
BROCHURES & TECHNICAL GUIDES
Get help with product selection, protocols, tips for optimization and troubleshooting.
Learn about NEB's recommended products for cloning.
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Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more about the benefits and disadvantages of Traditional Cloning.
In this webinar, we will explore some of the latest applications for NEBuilder HiFi DNA Assembly. View full Q & A summary.
Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments.
Primer Design and Fragment Assembly Using NEBuilder® HiFi DNA Assembly or Gibson Assembly® E5510 E5520
Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit.
How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.
The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how.
Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.
Follow these tips to get superior results.
NEB continues to develop and produce the most extensive commercially available selection of high-quality, and performance-optimized DNA ligases and ligase master mixes for your ligation needs.
Not sure why Q5® is your best choice for high-fidelity amplification of GC-rich targets? NEB's scientists will show you why we call Q5 an "ultra-high fidelity polymerase".
Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).