Restriction Enzyme Digestion Products

Restriction enzymes, first described in 1971, are bacterially derived enzymes that cleave DNA. Evolutionarily, restriction enzymes arose as a bacterial self-defense mechanism; the genomes of invading organisms would be degraded, leading to an inability to replicate. Type II restriction enzymes generally recognize an inverted repeat palindrome. This recognition site structure leads to a symmetrical cleavage of both DNA strands and results in either blunt- or sticky-ends of the digested DNA. Blunt ends are universally compatible with other blunt-ended DNA and possess a 5’ phosphate group to promote ligation. Sticky ends, on the other hand, are stretches of single-stranded DNA that is capable of self-ligation or ligation with a complementary region of DNA from another molecule or organism.

Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. 


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Restriction Enzyme Digestion
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AatII

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AbaSI

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Acc65I

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AccI

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AciI

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AclI

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AcuI

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AfeI

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AflII

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AflIII

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AgeI

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AgeI-HF®

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AhdI

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AleI-v2

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AluI

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AlwI

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AlwNI

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ApaI

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ApaLI

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ApeKI

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ApoI

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ApoI-HF®

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AscI

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AseI

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AsiSI

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AvaI

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AvaII

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AvrII

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BaeGI

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BaeI

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BamHI

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BamHI-HF®

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BanI

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BanII

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BbsI

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BbsI-HF®

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BbvCI

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BbvI

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BccI

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BceAI

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BcgI

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BciVI

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BclI

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BclI-HF

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BcoDI

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BfaI

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BfuAI

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BglI

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BglII

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BlpI

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BmgBI

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BmrI

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BmtI

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BmtI-HF®

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BpmI

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Bpu10I

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BpuEI

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BSA, Molecular Biology Grade

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BsaAI

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BsaBI

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BsaHI

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BsaI-HF®v2

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BsaJI

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BsaWI

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BsaXI

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BseRI

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BseYI

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BsgI

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BsiEI

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BsiHKAI

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BsiWI

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BslI

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BsmAI

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BsmBI-v2

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BsmFI

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BsmI

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BsoBI

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Bsp1286I

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BspCNI

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BspDI

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BspEI

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BspHI

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BspMI

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BspQI

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BsrBI

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BsrDI

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BsrFI-v2

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BsrGI

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BsrI

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BssHII

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BssSI-v2

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BstAPI

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BstBI

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BstEII

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BstEII-HF®

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BstNI

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BstUI

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BstYI

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Bsu36I

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BtgI

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BtgZI

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BtsCI

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BtsIMutI

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BtsI-v2

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Cac8I

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ClaI

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CspCI

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CutSmart® Buffer

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CviAII

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CviKI-1

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CviQI

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DdeI

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Diluent A

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Diluent B

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Diluent C

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DpnI

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DpnII

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DraI

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DraIII-HF®

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DrdI

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EaeI

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EagI-HF®

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EarI

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EciI

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Eco53kI

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EcoNI

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EcoO109I

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EcoP15I

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EcoRI

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EcoRI-HF®

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EcoRV

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EcoRV-HF®

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FatI

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FauI

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Fnu4HI

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FokI

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FseI

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FspEI

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FspI

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HaeII

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HaeIII

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HgaI

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HhaI

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HincII

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HindIII

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HindIII-HF®

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HinfI

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HinP1I

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HpaI

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HpaII

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HphI

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Hpy166II

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Hpy188I

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Hpy188III

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Hpy99I

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HpyCH4III

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HpyCH4IV

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HpyCH4V

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I-CeuI

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I-SceI

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KasI

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KpnI

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KpnI-HF®

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LpnPI

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MboI

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MboII

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McrBC

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MfeI

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MfeI-HF®

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MluCI

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MluI

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MluI-HF®

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MlyI

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MmeI

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MnlI

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MscI

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MseI

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MslI

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MspA1I

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MspI

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MspJI

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MwoI

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NaeI

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NarI

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NciI

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NcoI

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NcoI-HF®

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NdeI

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NEBuffer™ 1

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NEBuffer™ 1.1

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NEBuffer™ 2

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NEBuffer™ 2.1

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NEBuffer™ 3

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NEBuffer™ 3.1

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NEBuffer™ 4

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NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

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NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)

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NgoMIV

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NheI-HF®

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NlaIII

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NlaIV

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NmeAIII

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NotI

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NotI-HF®

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NruI

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NruI-HF®

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NsiI

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NsiI-HF®

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NspI

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PacI

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PaeR7I

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PciI

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PflFI

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PflMI

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PI-PspI

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PI-SceI

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PleI

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PmeI

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PmlI

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PpuMI

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PshAI

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PspGI

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PspOMI

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PspXI

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PstI-HF®

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PvuI

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PvuI-HF®

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PvuII

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PvuII-HF®

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rCutSmart™ Buffer

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Recombinant Albumin, Molecular Biology Grade

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RsaI

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RsrII

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SacI

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SacI-HF®

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SacII

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S-adenosylmethionine (SAM)

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SalI

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SalI-HF®

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SapI

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Sau3AI

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Sau96I

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SbfI

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SbfI-HF®

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ScaI-HF®

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ScrFI

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SexAI

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SfaNI

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SfcI

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SfiI

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SfoI

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SgrAI

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SmaI

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SmlI

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SnaBI

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SpeI

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SpeI-HF®

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SphI

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SphI-HF®

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SrfI

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SspI

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SspI-HF®

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StuI

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StyD4I

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StyI-HF®

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SwaI

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TaqI-v2

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TfiI

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TseI

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Tsp45I

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TspMI

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TspRI

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Tth111I

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XbaI

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XcmI

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XhoI

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XmaI

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XmnI

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ZraI