
DNA Preparation
Choose Type:
- Affinity Purification and On-column Cleavage (E6901)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Construction of the Fusion Plasmid (E6901)
- Fusion Protein Expression (E6901)
- Preparation of Media and Solutions (E6901)
- Primer Design for Restriction Enzyme Cloning (E6901)
- Simplified Expression and Purification Protocol (E6901)
- Optimizing Restriction Endonuclease Reactions
- Fusion Constructs (E6901)
- Protocol for Cre Recombinase (M0298)
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using MspJI, FspEI or LpnPI
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A Modern Day Gene Genie Sir Richard Roberts on Rebase
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Why Choose the K. lactis Protein Expression Kit?
Review the advantages of the K. lactis Protein Expression Kit for rapid, high yield protein expression in yeast.
- Molecular Cloning Technical Guide
- Cleavage Of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Recleavable Blunt Ends
- Recleavable Filled-in 5' Overhangs
- Why Choose Recombinant Enzymes?
- Troubleshooting Guide for Cloning
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Site Preferences
- Star Activity
- Traditional Cloning Quick Guide
- cDNA/Reverse Transcriptase Tips
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Shah, S., Sanchez, J., Stewart, A., et al. 2015. Probing the Run-On Oligomer of Activated SgrAI Bound to DNA PLoS One. 10(4), PubMedID: 25880668, DOI: 10.1371/journal.pone.0124783.
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