Non-Cloning Ligation

The ability to ligate two or more pieces of DNA together enzymatically has found utility in many applications in the life sciences. Library preparation for Next Generation Sequencing (NGS) typically incorporates a ligation step to add bar-coded adapters to fragmented DNA, a critical step in this popular workflow. Many novel detection methodologies incorporate the ligation of DNA probes followed by a polymerase-based amplification step. For example, single nucleotide polymorphism (SNP) detection using the Ligase Chain Reaction (LCR) is a well-known application using a non-cloning thermostable ligase. Many other detection methods capitalizing on the ligation of DNA are being employed by basic and applied research groups, as well as many molecular diagnostics (MDx) companies.

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Protocols for Non-Cloning Ligation
Application Notes Non-Cloning Ligation
    Publications related to Non-Cloning Ligation
  1. Anton, B.P., Morgan, R.D., Ezraty, B., Manta, B., Barras, F., Berkmen, M. 2019. Complete genome sequence of Escherichia coli BE104, an MC4100 drivative lacking the methionine reductive pathway Microbiol Resour Announc. 8 (29), PubMedID: 31296691, DOI: 10.1128/MRA.00721-19
  2. Potapov, V., Ong, J.L., Kucera, R.B., Langhorst, B.W., Bilotti, K., Pryor, J.M., Cantor, E.J., Canton, B., Knight, T.F., Evans, T.C., Lohman, G.J.S. 2018. Comprehensive profiling of four base overhang ligation fidelity by T4 DNA ligase and application to DNA assembly ACS Synthetic Biology. 7 (11), PubMedID: 30335370, DOI: 10.1021/acssynbio.8b00333
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