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    DNA End Treatment

    Modification of the termini of double-stranded DNA is often necessary to prepare the molecule for cloning. DNA ligases require a 5' monophosphate for adenylation of the donor end, while the acceptor end requires a 3' hydroxyl group. Additionally, the sequences to be joined need to be compatible: either a blunt end being joined to another blunt end, or a cohesive end with a complementary overhang to another cohesive end. End modifications are performed to improve the efficiency of the cloning process, ensure the ends to be joined are compatible, and to optimize the positioning of regulatory and translated sequences.

    Learn more about the various ways to modify DNA ends: Dephosphorylation, Blunting, Phosphorylation, A-tailing.

    Featured Products

    DNA End Treatment includes these areas of focus:

    Dephosphorylation
    Blunting
    Phosphorylation (Kinase)
    A-tailing

    FAQs for DNA End Treatment

    Common Applications of Exonucleases and Endonucleases

    NEB provides a list of common applications for our exonucleases and endonucleases.

    Properties of Exonucleases and Endonucleases

    NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.