- Molecular Cloning Technical Guide
- DNA Ligase Selection Chart
- Troubleshooting Guide for End Modification
- Troubleshooting Guide for Phosphorylation
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
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Phosphorylation is the process by which phosphate groups are added to a molecule by a kinase. The phosphorylation status of a fragment of DNA can influence its ability to proceed in reactions. Learn more about phosphorylation and kinases.
NEBioCalculator® - Using the ds: mass -> ends module to plan a phosphorylation reaction using T4 Polynucleotide Kinase (T4 PNK)
This tutorial describes the use of the NEBioCalculator web tool to convert linear dsDNA mass to moles of DNA ends for a phosphorylation reaction using T4 PNK