DNA End Modification
Modification of the termini of double-stranded DNA is often necessary to prepare the molecule for cloning. DNA ligases require a 5' monophosphate for adenylation of the donor end, while the acceptor end requires a 3' hydroxyl group. Additionally, the sequences to be joined need to be compatible: either a blunt end being joined to another blunt end, or a cohesive end with a complementary overhang to another cohesive end. End modifications are performed to improve the efficiency of the cloning process, ensure the ends to be joined are compatible, and to optimize the positioning of regulatory and translated sequences.
Learn more about the various ways to modify DNA ends: Dephosphorylation, Blunting, Phosphorylation, A-tailing.
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DNA End Modification includes these areas of focus:
FAQs for DNA End Modification
Protocols for DNA End Modification
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Molecular Cloning Technical Guide
Download the latest Molecular Cloning Technical Guide for help with product selection, protocols, tips for optimization and trouble-shooting.
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Reagents & Tools for Molecular Cloning brochure
Learn about recommended products for cloning in our Reagents and Tools for Molecular Cloning Brochure.
- A-tailing Selection Chart
- Blunting Selection Chart
- DNA Ligase Selection Chart
- Phosphatase Selection Chart
- Troubleshooting Guide for Cloning
- Troubleshooting Guide for End Modification
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
Other Tools & Resources
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
Common Applications of Exonucleases and Endonucleases
Properties of Exonucleases and Endonucleases
Legal Information
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
