Comparison of DNA Assembly Reaction Types
Return to NEBuilder® HiFi DNA AssemblyNEBuilder HiFi DNA Assembly offers error-free assembly that can be used for a wide range of reaction types. Furthermore, there are no licensing fee requirements from NEB for NEBuilder HiFi DNA Assembly products. See how it compares to NEB Gibson Assembly® and In-Fusion® HD.
| NEBuilder HiFi DNA Assembly | NEB Gibson Assembly | In-Fusion HD | ||||
|---|---|---|---|---|---|---|
| Assembly reaction types | Assembly efficiency | Covalently sealed?* | Assembly efficiency | Covalently sealed?* | Assembly efficiency | Covalently sealed?* |
| 2-fragment assembly | ||||||
| No mismatch | +++ | Yes | ++ | Yes | ++ | No |
| 3´- & 5´-end mismatch | +++ | Yes | ++ | Yes | X | No |
| 4-fragment assembly | ||||||
| 15-bp overlap & no mismatch |
+++ | Yes | ++ | Yes | ++ | No |
| 25-bp overlap & no mismatch |
+++ | Yes | ++ | Yes | ++ | No |
| Oligo assembly | ||||||
| 3´- and 5´-overhang | +++ | Yes | ++ | Yes | X | No |
| Blunt end & no mismatch |
+++ | Yes | ++ | Yes | X | No |
| ssOligo & vector | +++ | Yes | NP | Yes | X | No |
* Assembled products are treated with T5 exonuclease followed by PCR. Only covalently sealed products resistant to T5 exonuclease digestion can serve as templates for PCR and yield PCR product.
+++ Performs best; recommended
++ Performs well; but other product(s) perform better
+ Performs, but not recommended
X Does not perform
NP Experiment not performed
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
IN-FUSION® is a registered trademark of ClonTech Laboratories, Inc.
GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.
