NEBuilder HiFi DNA Assembly offers error-free assembly that can be used for a wide range of reaction types. Furthermore, there are no licensing fee requirements from NEB for NEBuilder HiFi DNA Assembly products. See how it compares to NEB Gibson Assembly® and In-Fusion® HD.
| |
NEBuilder HiFi DNA Assembly |
NEB Gibson Assembly |
In-Fusion HD |
| Assembly reaction types |
Assembly efficiency |
Covalently sealed?* |
Assembly efficiency |
Covalently sealed?* |
Assembly efficiency |
Covalently sealed?* |
| 2-fragment assembly |
| No mismatch |
+++ |
Yes |
++ |
Yes |
++ |
No |
| 3´- & 5´-end mismatch |
+++ |
Yes |
++ |
Yes |
X |
No |
| 4-fragment assembly |
15-bp overlap
& no mismatch |
+++ |
Yes |
++ |
Yes |
++ |
No |
25-bp overlap
& no mismatch |
+++ |
Yes |
++ |
Yes |
++ |
No |
| Oligo assembly |
| 3´- and 5´-overhang |
+++ |
Yes |
++ |
Yes |
X |
No |
Blunt end
& no mismatch |
+++ |
Yes |
++ |
Yes |
X |
No |
| ssOligo & vector |
+++ |
Yes |
NP |
Yes |
X |
No |
* Assembled products are treated with T5 exonuclease followed by PCR. Only covalently sealed products resistant to T5 exonuclease digestion can serve as templates for PCR and yield PCR product.
+++ Performs best; recommended
++ Performs well; but other product(s) perform better
+ Performs, but not recommended
X Does not perform
NP Experiment not performed
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
IN-FUSION® is a registered trademark of ClonTech Laboratories, Inc.
GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.
