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NEBuilder HiFi DNA Assembly offers error-free assembly that can be used for a wide range of reaction types. Furthermore, there are no licensing fee requirements from NEB for NEBuilder HiFi DNA Assembly products. See how it compares to NEB Gibson Assembly® and In-Fusion® HD.
NEBuilder HiFi DNA Assembly
NEB Gibson Assembly
In-Fusion HD
Assembly reactiontypes
Assemblyefficiency
Covalentlysealed?*
Assemblyefficiency
Covalentlysealed?*
Assemblyefficiency
Covalentlysealed?*
2-fragmentassembly
No mismatch
+++
Yes
++
Yes
++
No
3´- & 5´-end mismatch
+++
Yes
++
Yes
X
No
4-fragmentassembly
15-bp overlap
& no mismatch
+++
Yes
++
Yes
++
No
25-bp overlap
& no mismatch
+++
Yes
++
Yes
++
No
Oligoassembly
3´- and 5´-overhang
+++
Yes
++
Yes
X
No
Blunt end
& no mismatch
+++
Yes
++
Yes
X
No
ssOligo & vector
+++
Yes
NP
Yes
X
No
* Assembled products are treated with T5 exonuclease followed by PCR. Only covalently sealed products resistant to T5 exonuclease digestion can serve as templates for PCR and yield PCR product.
+++ Performs best; recommended
++ Performs well; but other product(s) perform better
+ Performs, but not recommended
X Does not perform
NP Experiment not performed
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
IN-FUSION® is a registered trademark of ClonTech Laboratories, Inc.
GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.
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