Studies of protein expression, interactions, and structure, often use reporter systems to introduce and select for gene targets in cells. Reporter genes confer drug resistance, bioluminescence or fluorescence properties into the cells they are introduced. Typical reporter studies link reporter genes directly to a promoter region of interest, the function of which can be monitored by the reporter activity. Protein fusion tagging is used to detect subcellular localization, degradation, protein-protein interactions, etc. Typical fusion tags are fluorescent proteins (e.g eGFP) or small protein epitopes (e.g FLAG, Myc HA) which can be detected by fluorescence FACS or western blots. New generations of reporter gene systems expand the range of applications and enhance experimental possibilities.
- Luciferase reporter genes with the enhanced properties of greater stability, robust secretory signal, smaller size, high sensitivity and no dependence on ATP are applicable for reporter gene studies, siRNA potency screening, live cell monitoring and BRET applications in vivo.
- Enhanced GFP and GFP color derivatives are useful for imaging mammalian cells, single fluorescent protein-based biosensors, FRET and producing transgenic model organisms. However GFP can be toxic to cells at high expression levels.
- Multimodality fusion reporter technologies employ vectors containing multiple reporter genes. These systems enable optimal analysis by multiple methods, such as fluorescence for single cell imaging, bioluminescence for small animal imaging, or short-lived radioactive tracers in living subjects for PET.
- Split reporter systems enable protein-protein interaction assays by cloning target genes into separate vectors, each linked to complementary portions of a reporter, such as luciferases or GFP.
- Cellular Labeling (E9100)
- Cellular Labeling (E9230)
- Labeling of Proteins in Solution (E9230)
- Use of SNAP-Cell Block with SNAP-Cell Substrates (E9100)
- Use of CLIP-Cell Block with CLIP-Cell Substrates (E9230)
- Western Analysis (E8023)
- Cellular Labeling (E9120)
- Instructions for Cellular Labeling (E9200)
- Labeling Proteins in vitro (E9120)
- Labeling Proteins in vitro (E9200)
- Cloning of SNAP-tag Fusions in pSNAPf (N9183)
- Expression of SNAPf Fusions (N9183)
- Labeling of Proteins in vitro (E9100)
Gaussia and Cypridina Luciferases - Ultrasensitive secreted reporters and their use in dual assays
- Reuter, W.H., Masuch, T., Ke, N., Lenon, M., Radzinski, M., Van Loi, V., Ren, G., Riggs, P., Antelmann, H., Reichmann, D., Leichert, L.I., Berkmen, M 2019. Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote Redox Biol. 26, PubMedID: 31450103, DOI: 10.1016/j.redox.2019.101280
- Carpinone, E.M., Li, Z., Mills, M.K., Foltz, C., Brannon, E.R., Carlow, C.K.S., Starai, V.J. 2018. Identification of putative effectors of the Type IV secretion system from the Wolbachia endosymbiont of Brugia malayi PLoS One. 13 (9), PubMedID: 30261054, DOI: 10.1371/journal.pone.0204736
|Does not require and
not affected by ATP
Comparison of the features of commercially available reporter systems highlights the advantages of using Gaussia or Cypridina Luciferase from NEB.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.