The non-overlapping substrate specificity of SNAP-tag® and CLIP-tag™ enables the orthogonal and complementary labeling of two proteins simultaneously in the same cell. Furthermore, SNAP-tag and CLIP-tag based pulse-chase experiments allow for visualization of different generations of proteins and analysis of protein turnover in living cells (1-3). Additionally, there are a numbers of companion products designed to enable a wide variety of other applications including: Biotin substrates, SDS-PAGE specific SNAP-tag and CLIP-Vista labels, non-fluorescent blocking agents, resin and magnetic beads for pull-down applications, building blocks for the generation of custom substrates, and a SNAP-tag specific purified protein and antibody.
- Jansen, L. et al (2007) J.Cell. Biol. 176, 795-805. PMID: 17339380
- Farr, G.A. (2009) J. Cell. Biol. 186, 269-282. PMID: 19620635
- Milenkovic, L. (2010) J. Cell. Biol. 187, 365-374. PMID: 19948480
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag™ is a trademark of New England Biolabs, inc.
FAQs for SNAP Companion
Protocols for SNAP Companion
- Labeling Mammalian Cell Lysates (S9147)
- Labeling Proteins in vitro (S9147)
- Labeling SNAP-tag Purified Protein In Vitro (P9312)
- Protocol for Anti-SNAP-tag® Antibody (Polyclonal) (P9310)
- Use SNAP-Capture Magnetic Beads (S9145)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag®
- Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Simultaneous Labeling of Two Proteins in Live Cells
- SNAPf based pulse labeling for analysis of protein turnover in living cells
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
SNAP-tag® Technologies: Novel Tools to Study Protein Function
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
- Simultaneous dual protein labeling inside live cells
- Protein localization and translocation
- Pulse-chase experiments
- Receptor internalization studies
- Selective cell surface labeling
- Protein pull-down assays
- Protein detection in SDS-PAGE
- Flow cytometry
- High throughput binding assays in microtiter plates
- Biosensor interaction experiments
- FRET-based binding assays
- Single molecule labeling
- Super-resolution microscopy
Protein labeling with SNAP-tag and CLIP-tag
SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.