The non-overlapping substrate specificity of SNAP-tag® and CLIP-tag™ enables the orthogonal and complementary labeling of two proteins simultaneously in the same cell. Furthermore, SNAP-tag and CLIP-tag based pulse-chase experiments allow for visualization of different generations of proteins and analysis of protein turnover in living cells (1-3). Additionally, there are a numbers of companion products designed to enable a wide variety of other applications including: Biotin substrates, SDS-PAGE specific SNAP-tag and CLIP-Vista labels, non-fluorescent blocking agents, resin and magnetic beads for pull-down applications, building blocks for the generation of custom substrates, and a SNAP-tag specific purified protein and antibody.
- Jansen, L. et al (2007) J.Cell. Biol. 176, 795-805. PMID: 17339380
- Farr, G.A. (2009) J. Cell. Biol. 186, 269-282. PMID: 19620635
- Milenkovic, L. (2010) J. Cell. Biol. 187, 365-374. PMID: 19948480
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag™ is a trademark of New England Biolabs, inc.
FAQs for SNAP Companion
Protocols for SNAP Companion
- Labeling Mammalian Cell Lysates (S9147)
- Labeling Proteins in vitro (S9147)
- Labeling SNAP-tag Purified Protein In Vitro (P9312)
- Protocol for Anti-SNAP-tag® Antibody (Polyclonal) (P9310)
- Use SNAP-Capture Magnetic Beads (S9145)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
Application Notes SNAP Companion
- Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag®
- Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Simultaneous Labeling of Two Proteins in Live Cells
- SNAPf based pulse labeling for analysis of protein turnover in living cells
SNAP-tag® Technologies: Novel Tools to Study Protein Function
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
- Simultaneous dual protein labeling inside live cells
- Protein localization and translocation
- Pulse-chase experiments
- Receptor internalization studies
- Selective cell surface labeling
- Protein pull-down assays
- Protein detection in SDS-PAGE
- Flow cytometry
- High throughput binding assays in microtiter plates
- Biosensor interaction experiments
- FRET-based binding assays
- Single molecule labeling
- Super-resolution microscopy
Protein labeling with SNAP-tag and CLIP-tag
SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.