Membrane proteins are challenging to study given their hydrophobic nature, generally low native abundance and intrinsic instability(1,2). Regardless, half of all protein drug targets are membrane proteins. For imaging, most fluorescent proteins (i.e. GFP) cannot specifically visualize cell surface subpopulations. However, CLIP-tag, SNAP-tag and cell surface-specific ACP/MCP-tag systems can specifically label subpopulations of target protein expressed on the cell surface using non-cell permeable substrates (3). This approach permits discrimination of different populations of a cell surface protein: those properly translocated to the plasma membrane from those retained in the secretory pathway or already internalized (e.g. upon ligand binding).
- Lacapère J-J, Pebay-Peyroula E, Neumann J-M, Etchebest C. (2007) Trends Biochem Sci. 32, 259–270. PMID: 17481903
- von Heijne G. (2007) J Intern Med. 261, 543–557. PMID: 17547710
- Keppler, A., Pick, H., Arrivoli, C. et al. (2004) Proc. Natl. Acad. Sci. USA, 101, 9955. PMID: 15226507
CLIP-tag™ is a trademark of New England Biolabs, Inc.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
- Cellular Labeling (E9230)
- Cellular Labeling (S9217)
- Cellular Labeling (S9219)
- Cellular Labeling (S9232)
- Cellular Labeling (S9233)
- Cellular Labeling (S9234)
- Cloning of CLIP-tag Fusions in pCLIPf (N9215)
- Expression of CLIP-tag Fusions (N9215)
- Labeling of Proteins in vitro (S9217)
- Labeling of Proteins in vitro (S9219)
- Labeling of Proteins in vitro (S9220)
- Labeling of Proteins in vitro (S9232)
- Labeling of Proteins in vitro (S9233)
- Labeling of Proteins in vitro (S9234)
- Labeling of Proteins in Solution (E9230)
- Use of CLIP-Cell Block with CLIP-Cell Substrates (E9230)
- Use with CLIP-tag substrates (S9220)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
- Cellular Imaging & Analysis Brochure
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- SNAP-tag® and CLIP-tag™ Substrate Selection Chart
- Labeling with SNAP-tag® Technology Troubleshooting Guide
- Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A (2019) A ligand based system for receptor specific delivery of proteins Sci Rep; 9(1), 19214.. PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
- Simultaneous dual protein labeling on the surface of live cells
- Protein localization and translocation
- Pulse-chase experiments
- Receptor internalization studies
- Selective cell surface labeling
- Protein detection in SDS-PAGE
- Flow cytometry
- High throughput binding assays in microtiter plates
- Biosensor interaction experiments
- FRET-based binding assays
- Single molecule labeling
- Super-resolution microscopy
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.