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CLIP Surface

Membrane proteins are challenging to study given their hydrophobic nature, generally low native abundance and intrinsic instability(1,2).  Regardless, half of all protein drug targets are membrane proteins.  For imaging, most fluorescent proteins (i.e. GFP) cannot specifically visualize cell surface subpopulations.  However, CLIP-tag, SNAP-tag and cell surface-specific ACP/MCP-tag systems can specifically label subpopulations of target protein expressed on the cell surface using non-cell permeable substrates (3).  This approach permits discrimination of different populations of a cell surface protein: those properly translocated to the plasma membrane from those retained in the secretory pathway or already internalized (e.g. upon ligand binding).

References

  1. Lacapère J-J, Pebay-Peyroula E, Neumann J-M, Etchebest C. (2007) Trends Biochem Sci. 32, 259–270. PMID: 17481903
  2. von Heijne G. (2007) J Intern Med. 261, 543–557. PMID: 17547710
  3. Keppler, A., Pick, H., Arrivoli, C. et al. (2004) Proc. Natl. Acad. Sci. USA, 101, 9955. PMID: 15226507

CLIP-tag™ is a trademark of New England Biolabs, Inc.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.

FAQs for CLIP Surface

Protocols for CLIP Surface

Features

  • Clone and express once, then use with a variety of substrates
  • Non-toxic to living cells
  • Wide selection of fluorescent substrates
  • Highly specific covalent labeling
  • Simultaneous dual labeling

Applications

  • Simultaneous dual protein labeling on the surface of live cells
  • Protein localization and translocation
  • Pulse-chase experiments
  • Receptor internalization studies
  • Selective cell surface labeling
  • Protein detection in SDS-PAGE
  • Flow cytometry
  • High throughput binding assays in microtiter plates
  • Biosensor interaction experiments
  • FRET-based binding assays
  • Single molecule labeling
  • Super-resolution microscopy

Protein Labeling with SNAP-tag and CLIP-tag

The SNAP- (gold) or CLIP-tag (purple) is fused to the protein of interest (blue). Labeling occurs through covalent attachment to the tag, releasing either a guanine or a cytosine moiety.

SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart

NEB offers a large selection of fluorescent labels (substrates) for SNAP-, CLIP-, ACP- and MCP-tag fusion proteins.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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