LOCUS pSV40-GLuc 4178 bp DNA circular 24-SEP-2012 DEFINITION Cloning vector pSV40-GLuc, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pSV40-GLuc ORGANISM Cloning vector pSV40-GLuc Unclassified. REFERENCE 1 (bases 1 to 4178) AUTHORS Tzertzinis,G. TITLE Direct Submission JOURNAL Submitted (24-SEP-2012) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4178 /organism="Cloning vector pSV40-GLuc" /mol_type="other DNA" misc_feature 1..51 /note="multiple cloning site (BamHI-HindIII)" promoter 52..247 /gene="GLuc" /note="SV40 promoter (clockwise)" gene 306..863 /gene="GLuc" CDS 306..863 /gene="GLuc" /codon_start=1 /product="Gaussia princeps luciferase" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADR GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKE SAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKW LPQRCATFASKIQGQVDKIKGAGGD" sig_peptide 306..356 /gene="GLuc" /note="secretory signal sequence" polyA_signal 889..1110 /gene="GLuc" /note="SV40 late polyA" enhancer 1117..1363 /gene="GLuc" /note="SV40 enhancer" rep_origin complement(1681..2269) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(2440..3300) /gene="bla" CDS complement(2440..3300) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3432..3877 /note="f1 origin of replication (+ -)" BASE COUNT 1023 a 1066 c 1041 g 1048 t ORIGIN 1 ggtaccaccg atcgatcgct agctgcagag ctcgaattcg atatcaagct tgcatctcaa 61 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 121 ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag aggccgaggc 181 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 241 ttgcaactcg agattccggt actgttggta aactaagctt ggtaccgagc tcggatccag 301 ccaccatggg agtcaaagtt ctgtttgccc tgatctgcat cgctgtggcc gaggccaagc 361 ccaccgagaa caacgaagac ttcaacatcg tggccgtggc cagcaacttc gcgaccacgg 421 atctcgatgc tgaccgcggg aagttgcccg gcaagaagct gccgctggag gtgctcaaag 481 agatggaagc caatgcccgg aaagctggct gcaccagggg ctgtctgatc tgcctgtccc 541 acatcaagtg cacgcccaag atgaagaagt tcatcccagg acgctgccac acctacgaag 601 gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat cgtcgacatt cctgagattc 661 ctgggttcaa ggacttggag cccatggagc agttcatcgc acaggtcgat ctgtgtgtgg 721 actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttctgac ctgctcaaga 781 agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag gtggacaaga 841 tcaagggggc cggtggtgac taagcggccg ctcgagcatg catctagaca gacatgataa 901 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 961 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 1021 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 1081 aaagcaagta aaacctctac aaatgtggta ggatccgata aggatctgaa cgatggagcg 1141 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta 1201 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg 1261 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 1321 gggagcctgg ggactttcca caccctaact gacacacatt ccaggatccg tcgaccgatg 1381 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc 1441 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 1501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 1561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 1621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 1741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 1801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 1861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 1921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 1981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 2161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 2461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 2521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 2581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 2641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 2701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 2761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 2821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 2881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 2941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 3181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 3241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 3301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 3361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 3421 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 3481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 3541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 3601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 3661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 3721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 3781 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 3841 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt 3901 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc 3961 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat 4021 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta 4081 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc 4141 agtgcaagtg caggtgccag aacatttctc tatcgata //