LOCUS pNEBR-R1Puro 9337 bp DNA circular 07-AUG-2008 DEFINITION Cloning vector pNEBR-R1Puro, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pNEBR-R1Puro ORGANISM Cloning vector pNEBR-R1Puro other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9337) AUTHORS Maina,C.V. TITLE Direct Submission JOURNAL Submitted (07-AUG-2008) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..9337 /organism="Cloning vector pNEBR-R1Puro" /mol_type="other DNA" gene complement(9..3622) /gene="RheoReceptor" polyA_signal complement(9..520) /gene="RheoReceptor" /note="TK polyA (counter-clockwise)" CDS complement(551..2017) /gene="RheoReceptor" /codon_start=1 /product="RheoReceptor" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRPECVVP ETQCAMKRKEKKAQKEKDKLPVSTTTVDDHMPPIMQCEPPPPEAARIHEVVPRFLSDK LLVTNRQKNIPQLTANQQFLIARLIWYQDGYEQPSDEDLKRITQTWQQADDENEESDT PFRQITEMTILTVQLIVEFAKGLPGFAKISQPDQITLLKACSSEVMMLRVARRYDAAS DSILFANNQAYTRDNYRKAGMAEVIEDLLHFCRCMYSMALDNIHYALLTAVVIFSDRP GLEQPQLVEEIQRYYLNTLRIYILNQLSGSARSSVIYGKILSILSELRTLGMQNSNMC ISLKLKNRKLPPFLEEIWDVADMSHTQPPPILESPTNL" promoter complement(2033..3622) /gene="RheoReceptor" /note="UbB promoter (counter-clockwise)" gene 3631..6081 /gene="RheoActivator" promoter 3631..4844 /gene="RheoActivator" /note="UbC promoter (clockwise)" CDS 4863..5837 /gene="RheoActivator" /codon_start=1 /product="RheoActivator" /translation="MGPKKKRKVAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDML GDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGGEFEMPVDRILEAELA VEQKSDQGVEGPGGTGGSGSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSSLPLDDQ VILLRAGWNELLIASFSHRSIDVRDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVS KMRDMRMDKTELGCLRAIILFNPEVRGLKSAQEVELLREKVYAALEEYTRTTHPDEPG RFAKLLLRLPSLRSIGLKCLEHLFFFRLIGDVPIDTFLMEMLESPSDS" polyA_signal 5844..6081 /gene="RheoActivator" /note="SV40 late polyA (clockwise)" gene 6088..7149 /gene="pac" promoter 6088..6453 /gene="pac" /note="SV40 enhancer/early promoter (clockwise)" CDS 6506..7105 /gene="pac" /note="purR (confers resistance to puromycin)" /codon_start=1 /product="puromycin acetyltransferase from Streptomyces alboniger" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 7106..7149 /gene="pac" /note="synthetic polyA (clockwise)" gene 7437..8297 /gene="bla" CDS 7437..8297 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 8368..9056 /note="pUC19 origin of replication (RNAII -35 to RNA/DNA switch point)" BASE COUNT 2065 a 2517 c 2509 g 2246 t ORIGIN 1 atttaaatcg ccagaaatcc gcgcggtggt ttttgggggt cgggggtgtt tggcagccac 61 agacgcccgg tgttcgtgtc gcgccagtac atgcggtcca tgcccaggcc atccaaaaac 121 catgggtctg tctgctcagt ccagtcgtgg acctgacccc acgcaacgcc caaaataata 181 acccccacga accataaacc attccccatg ggggaccccg tccctaaccc acggggccag 241 tggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac 301 ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg 361 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc 421 caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt 481 attgtctcct tccgtgtttc agttagcctc ccccatctcc ctgcagcccg ggggatcgaa 541 aggcggccgc ctagagattc gtgggggact cgaggatagg cggcggttgg gtgtgcgaca 601 tgtccgccac atcccagatc tcctcgagga aaggcggcag ctttctgttc ttgagcttga 661 gggagatgca catgttggag ttttgcatgc cgagcgtgcg tagctcagag aggattgaga 721 ggatcttgcc gtatatgacg gacgaacgcg ccgacccgct cagctggttc aggatataga 781 tgcggagcgt attcaggtag taccgctgga tctcttccac cagttgcggc tgctccaacc 841 ctggccggtc agaaaagatg acgacagccg tgagcagcgc gtaatggatg ttgtccaacg 901 ccatagagta catgcaccgg cagaagtgca gtagatcctc gatgacctcg gccatgccag 961 ccttgcggta gttgtcgcga gtgtacgctt ggttgttcgc gaacagaata ctgtctgagg 1021 ccgcatcgta tcgtcgcgcg actcggagca tcattacctc acttgagcaa gccttaagca 1081 gcgtaatttg atcaggctgc gagatcttgg cgaaccctgg caatcccttc gcgaactcca 1141 cgataagttg gaccgtgagg atagtcatct ctgtgatctg gcggaaggga gtgtccgact 1201 cttcgttttc atcgtccgct tgctgccacg tctgcgtaat cctcttcaaa tcttcatcag 1261 aaggctgctc gtacccgtcc tggtaccaga tgagcctggc gataaggaac tgctggttgg 1321 ctgtcaactg ggggatgttt ttctgccggt ttgtcaccaa cagcttgtcg gagagaaacc 1381 ttgggaccac ttcgtgaatc cttgctgctt caggaggtgg aggttcacac tgcataatgg 1441 gcggcatgtg gtcgtccacc gtcgtcgtgc tgacaggcag tttgtccttc tccttctgtg 1501 ctttcttctc tttccgcttc atggcgcact gagtctcggg tactacgcac tcaggccgga 1561 tccccgggaa ttccggcgat acagtcaact gtctttgacc tttgttacta ctctcttccg 1621 atgatgatgt cgcacttatt ctatgctgtc tcaatgttag aggcatatca gtctccactg 1681 aagccaatct atctgtgacg gcatctttat tcacattatc ttgtacaaat aatcctgtta 1741 acaatgcttt tatatcctgt aaagaatcca ttttcaaaat catgtcaagg tcttctcgag 1801 gaaaaatcag tagaaatagc tgttccagtc tttctagcct tgattccact tctgtcagat 1861 gtgccctagt cagcggagac cttttggttt tgggagagta gcgacactcc cagttgttct 1921 tcagacactt ggcgcacttc ggtttttctt tggagcactt gagcttttta agtcggcaaa 1981 tatcgcatgc ttgttcgata gaagacagta gcttcatggt ggcatcgcta gccactaaga 2041 tctgcatttg acctagttta aaagtaaaac ataagcgtgt cacctcagga tcattgtatt 2101 tcttgctttt acacctaata ttcttcaaaa ttagcaacta ttccttcaaa atgccttaaa 2161 tacgttaata ttccgcaagc ctcccctcaa ttttacaagg ctaagtcact taatacgaaa 2221 ttcgtcattc aaggtttcaa cgtcaacagc ggaaaatcca gagcgctctc ttaccaagcg 2281 tccacagctt tccccactaa ctgttccgga tattcaaact cccccgaccg aactccaatc 2341 tgcctcaaac ctaagcacgg tgttaggcaa actgaaaaca aaaccaaacc cacaagactc 2401 gcgaaataaa aatcccattg aaacacaaga aggggcacct ttctcacact gggattccaa 2461 agtaaaggct tccagaaacc acacaaacta cctcttttcc gccttccccg cgaagtaaac 2521 tcgacagaat cgccgatcgg ttaggcgtcc gttagtttcc gtcaacgcgc gcgcgcgcca 2581 accgccctcg cccccctcag gtcaacaagg cccactgtcc cggaaaggcc cagcgccgcg 2641 tcacctcgcc ctcccctccc ccacgccctc ccctcagcgg cccacccaac cccaagaagc 2701 ctccaccttg acctcaccca ttcccccctc tcacaccccc ccaaggtcgt tacggctgcg 2761 ggccagtacc tgttagcgga taccaggatc ctgccaatca ccaaccacgt ccacccacag 2821 ggacacaaac aagctcaccc aacaaagcca accgccccta aatgctccgg gctggcggag 2881 cgcaaattta tgcaacgagt gttgcgtcac ttatcacccc tcacgtagac gccgtctccg 2941 tgcgccgctg aaagtagttc gccgcgccca acctcccgag aggcagttta ggcttttcaa 3001 ctgagcccca aattcctcat agccgtaaga aaggctccta aaaaattatt tcccttctcc 3061 tctatgcgct tctcttcctt ctttaggaaa atataagaaa attatcaggc ggatgaattt 3121 gagttgaaga aaccttcctc gaaagctctg gaaacttcct tgcagcgccg gcactgattg 3181 gttaggcctg cgaaggcgag gtcggcgctg attggtgggg gacgcggtgg ctctgttgtt 3241 gctgggctcc acctggggtg gcttagcgcc gaggttgcta agtaactgag cgcgcgtgcg 3301 ccgcacaggt ctttcctttt ttttcttgag acggagtttc gctcttgttt cccaggctgg 3361 agtgcaatgg cgcgatctcg gctcatcgca acctccgcct cccgggttca agcgattctc 3421 ctgcctcagc ctcccaagta gctgggatta caggcatgcg ccaccacgtc cggctaattt 3481 tttgtatttt tagtagagac gggggtttca ccatgtttgt caggctggtc tcgaactcct 3541 gacctcaggt aatccacccg cctcggcctg ccaaagtggg ccgccgcgcc tcagaagcgg 3601 gacggcgtgg cacccgatat cgggcgcgcc ggcctccgcg ccgggttttg gcgcctcccg 3661 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc 3721 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag 3781 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg 3841 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg 3901 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac 3961 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc 4021 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct 4081 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag 4141 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg 4201 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg 4261 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg 4321 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact 4381 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt 4441 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat 4501 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag 4561 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag 4621 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg 4681 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag 4741 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa 4801 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaatcgct agcgccgcca 4861 ccatgggccc taaaaagaag cgtaaagtcg cccccccgac cgatgtcagc ctgggggacg 4921 agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg 4981 atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg 5041 ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc 5101 ttggaattga cgagtacggt ggggaattcg agatgcctgt ggacaggatc ctggaggcag 5161 agcttgctgt ggaacagaag agtgaccagg gcgttgaggg tcctggggga accgggggta 5221 gcggcagcag cccaaatgac cctgtgacta acatctgtca ggcagctgac aaacagctat 5281 tcacgcttgt tgagtgggcg aagaggatcc cacacttttc ctccttgcct ctggatgatc 5341 aggtcatatt gctgcgggca ggctggaatg aactcctcat tgcctccttt tcacaccgat 5401 ccattgatgt tcgagatggc atcctccttg ccacaggtct tcacgtgcac cgcaactcag 5461 cccattcagc aggagtagga gccatctttg atcgggtgct gacagagcta gtgtccaaaa 5521 tgcgtgacat gaggatggac aagacagagc ttggctgcct gagggcaatc attctgttta 5581 atccagaggt gaggggtttg aaatccgccc aggaagttga acttctacgt gaaaaagtat 5641 atgccgcttt ggaagaatat actagaacaa cacatcccga tgaaccagga agatttgcaa 5701 aacttttgct tcgtctgcct tctttacgtt ccataggcct taagtgtttg gagcatttgt 5761 ttttctttcg ccttattgga gatgttccaa ttgatacgtt cctgatggag atgcttgaat 5821 caccttctga ttcataatct agacgagcag acatgataag atacattgat gagtttggac 5881 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg 5941 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt 6001 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca 6061 aatgtggtaa aatccgataa gcgtaccttc tgaggcggaa agaaccagct gtggaatgtg 6121 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 6181 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 6241 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 6301 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 6361 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 6421 ttttttggag gcctaggctt ttgcaaaaag cttgattctt ctgacacaac agtctcgaac 6481 ttaaggctag aggccggccg ccaccatgac cgagtacaag cccacggtgc gcctcgccac 6541 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 6601 cacgcgccac accgtagacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 6661 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgctgc 6721 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 6781 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 6841 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 6901 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 6961 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 7021 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 7081 catgacccgc aagcccggtg cctgaaataa aatatcttta ttttcattac atctgtgtgt 7141 tggtttttta tttaaatttt ctaaatacat tcaaatatgt atccgctctg agctgcatgt 7201 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 7261 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 7321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 7381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 7441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 7501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 7561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 7621 agcgttctcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 7681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 7741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 7801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgataggag 7861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 7921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 7981 tagcaatggc aacaaccttg cgcaaactat taactggcga actacttact ctagcttccc 8041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 8101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 8161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 8221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 8281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 8341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgtcca 8401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 8461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 8521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 8581 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 8641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 8701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 8761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 8821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 8881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 8941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 9001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 9061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 9121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 9181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 9241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 9301 acaggtttcc cgactggaaa gcgggcagtg agcgcaa //