LOCUS pGGA 2174 bp DNA circular 07-FEB-2017 DEFINITION Cloning vector pGGA, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pGGA ORGANISM Cloning vector pGGA Unclassified. REFERENCE 1 (bases 1 to 2174) AUTHORS Kucera,R.B. TITLE Direct Submission JOURNAL Submitted (07-FEB-2017) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..2174 /organism="Cloning vector pGGA" /mol_type="other DNA" primer_bind 234..260 /note="GG assembly forward analysis primer sequence" regulatory 254..271 /regulatory_class="promoter" /note="SP6 promoter (transcript reverse)" misc_feature 284..301 /note="upstream multiple cloning site (XhoI-EcoRI)" misc_feature 315..318 /note="4 base BsaI overhang created during Golden Gate assembly (BsaI site 320-325)" misc_feature 319..355 /note="DNA sequence removed and replaced during Golden Gate assembly" misc_feature 356..359 /note="4 base BsaI overhang created during Golden Gate assembly (BsaI site 349-354)" misc_feature 373..406 /note="downstream multiple cloning site (PacI-NotI)" primer_bind complement(411..436) /note="GG assembly reverse analysis primer sequence" regulatory complement(419..436) /regulatory_class="promoter" /note="T7 promoter (transcript forward)" gene complement(547..1206) /gene="cat" CDS complement(547..1206) /gene="cat" /note="camR (confers resistance to chloramphenicol)" /codon_start=1 /product="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" rep_origin 1410..1998 /note="pUC19 origin of replication () (RNAII -35 to RNA/DNA switch point)" BASE COUNT 579 a 533 c 500 g 562 t ORIGIN 1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt 61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg 121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag 181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg 241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt 301 cgaagacttg gtacggagcg agaccgcttt ccagatctga taacttgtgg tctcaccatt 361 cctgtagtct tcttaattaa gacgtcagaa ttctcgaggc ggccgcatgt gcgtctccct 421 atagtgagtc gtattaattt cgcgggcgga acccctattt gtttattttt ctaaatacat 481 tcaaatatgt atccgctcat gagtagcacc aggcgtttaa gggcaccaat aactgcctta 541 aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct 601 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac 661 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat 721 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa 781 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc 841 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga 901 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac 961 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag 1021 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc 1081 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc 1141 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt 1201 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag 1261 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt 1321 tcgccaaaag ttgtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 1381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 1441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 1501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 1561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 1621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 1681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 1741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 1801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 1861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 1921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 1981 gggcggagcc tatggaaaaa cgccagcaat gcggcctttt tacggttcct ggccttttgc 2041 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 2101 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 2161 gtgagcgagg aagc //