LOCUS FLuc_Control_Plasmid 3925 bp DNA circular 09-SEP-2014 DEFINITION Cloning vector FLuc_Control_Plasmid, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector FLuc_Control_Plasmid ORGANISM Cloning vector FLuc_Control_Plasmid Unclassified. REFERENCE 1 (bases 1 to 3925) AUTHORS Chong,R. TITLE Direct Submission JOURNAL Submitted (09-SEP-2014) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..3925 /organism="Cloning vector FLuc_Control_Plasmid" /mol_type="other DNA" promoter 26..43 /note="T7 promoter (transcript start 43 clockwise)" gene 88..1791 /gene="FLuc" CDS 88..1791 /gene="FLuc" /codon_start=1 /product="Photinus pyralis (firefly) luciferase" /translation="MKIEEGKGHHHHHHGSMEDAKNIKKGPAPFYPLEDGTAGEQLHK AMKRYALVPGTIAFTDAHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSL QFFMPVLGALFIGVAVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLP IIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGST GLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGF RVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAP LSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDL DTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFI VDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKT MTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL E" terminator 1861..1983 /note="T7 Tphi transcription terminator" rep_origin complement(2182..2770) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point); single base point mutation (pUC19 1189 G -> pFLuc 2504 C)" gene complement(2942..3802) /gene="bla" CDS complement(2942..3802) /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant V82I,A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1066 a 912 c 953 g 994 t ORIGIN 1 gctagtggtg ctagccccgc gaaattaata cgactcacta tagggtctag aaataatttt 61 gtttaacttt aagaaggaga tataaccatg aaaatcgaag aaggtaaagg tcaccatcac 121 catcaccacg gatccatgga agacgccaaa aacataaaga aaggcccggc gccattctat 181 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg 241 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa 301 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat 361 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg 421 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc 481 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa 541 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa 601 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt 661 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata 721 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc 781 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact 841 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat 901 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga 961 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc 1021 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg 1081 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg 1141 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg 1201 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat 1261 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct 1321 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat 1381 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt 1441 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa 1501 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat 1561 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa 1621 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga 1681 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc 1741 agagagatcc tcataaaggc caagaagggc ggaaagtcca aactcgagta aggttaacct 1801 gcaggaggcc tttaattaag gtggtgcggc cgcgctagcg gtcccggggg atcgatccgg 1861 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag 1921 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta 1981 tatccggaag cttggcactg gccgaccggg gtcgagcact gactcgctgc gctcggtcgt 2041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 2461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccgctaagac acgacttatc 2521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 2581 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 2641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 2701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 2761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 2821 ctcacagatc cgggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 2881 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 2941 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 3001 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 3061 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 3121 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 3181 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 3241 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 3301 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 3361 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 3421 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 3481 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 3541 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 3601 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 3661 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 3721 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 3781 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 3841 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 3901 ttccgcgcac atttccccga aaagt //