LOCUS pCMV-CLuc_2 6905 bp DNA circular 09-JUL-2010 DEFINITION Cloning vector pCMV-CLuc_2, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pCMV-CLuc_2 ORGANISM Cloning vector pCMV-CLuc_2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6905) AUTHORS Shea,C. and Tzertzinis,G. TITLE Direct Submission JOURNAL Submitted (09-JUL-2010) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..6905 /organism="Cloning vector pCMV-CLuc_2" /mol_type="other DNA" promoter 209..863 /note="CMV promoter (clockwise)" promoter 863..880 /note="T7 promoter" misc_RNA 913..922 /note="Kozak consensus sequence for CLuc" gene 919..2580 /gene="CLuc" CDS 919..2580 /gene="CLuc" /codon_start=1 /product="Cypridina noctiluca luciferase" /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECID SSCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTY VLGQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADP IIANPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLC GDLKMMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPI NFYYYTISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQ FQGPCKEILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVG GEAVSVPYSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGIC GNYNQDFSDDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHK PDRVERCMYEYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK" promoter 3223..3558 /note="SV40 promoter (clockwise)" gene 3610..4404 /gene="aph(3')-II" CDS 3610..4404 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(4797..4826) /note="Plac promoter (-35 signal TTTACA, -10 signal TATGTT)" rep_origin complement(5150..5738) /note="pUC19 origin of replication () (RNAII -35 to RNA/DNA switch point) (old sequence errors)" gene complement(5909..6769) /gene="bla" CDS complement(5909..6769) /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant V82I,A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1691 a 1752 c 1730 g 1732 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccgccaccat gaagacctta attcttgccg ttgcattagt ctactgcgcc 961 actgttcatt gccaggactg tccttacgaa cctgatccac caaacacagt tccaacttcc 1021 tgtgaagcta aagaaggaga atgtattgat agcagctgtg gcacctgcac gagagacata 1081 ctatcagatg gactgtgtga aaataaacca ggaaaaacat gttgccgaat gtgtcagtat 1141 gtaattgaat gcagagtaga ggccgcagga tggtttagaa cattctatgg aaagagattc 1201 cagttccagg aacctggtac atacgtgttg ggtcaaggaa ccaagggcgg cgactggaag 1261 gtgtccatca ccctggagaa cctggatgga accaaggggg ctgtgctgac caagacaaga 1321 ctggaagtgg ctggagacat cattgacatc gctcaagcta ctgagaatcc catcactgta 1381 aacggtggag ctgaccctat catcgccaac ccgtacacca tcggcgaggt caccatcgct 1441 gttgttgaga tgccaggctt caacatcacc gtcattgagt tcttcaaact gatcgtgatc 1501 gacatcctcg gaggaagatc tgtaagaatc gccccagaca cagcaaacaa aggaatgatc 1561 tctggcctct gtggagatct taaaatgatg gaagatacag acttcacttc agatccagaa 1621 caactcgcta ttcagcctaa gatcaaccag gagtttgacg gttgtccact ctatggaaat 1681 cctgatgacg ttgcatactg caaaggtctt ctggagccgt acaaggacag ctgccgcaac 1741 cccatcaact tctactacta caccatctcc tgcgccttcg cccgctgtat gggtggagac 1801 gagcgagcct cacacgtgct gcttgactac agggagacgt gcgctgctcc cgaaactaga 1861 ggaacctgcg ttttgtctgg acatactttc tacgatacat ttgacaaagc aagataccaa 1921 ttccagggtc cctgcaagga gattcttatg gccgccgact gtttctggaa cacttgggat 1981 gtgaaggttt cacacaggaa tgttgactct tacactgaag tagagaaagt acgaatcagg 2041 aaacaatcga ctgtagtaga actcattgtt gatggaaaac agattctggt tggaggagaa 2101 gccgtgtccg tcccgtacag ctctcagaac acttccatct actggcaaga tggtgacata 2161 ctgactacag ccatcctacc tgaagctctg gtggtcaagt tcaacttcaa gcaactgctc 2221 gtcgtacata ttagagatcc attcgatggt aagacttgcg gtatttgcgg taactacaac 2281 caggatttca gtgatgattc ttttgatgct gaaggagcct gtgatctgac ccccaaccca 2341 ccgggatgca ccgaagaaca gaaacctgaa gctgaacgac tctgcaatag tctcttcgcc 2401 ggtcaaagtg atcttgatca gaaatgtaac gtgtgccaca agcctgaccg tgtcgaacga 2461 tgcatgtacg agtattgcct gaggggacaa cagggtttct gtgaccacgc atgggagttc 2521 aagaaagaat gctacataaa gcatggagac accctagaag taccagatga atgcaaatag 2581 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgtct 2641 agaaataatt cttactgtca tgccaagtaa gatgcttttc tgtgctgcaa tagcaggcat 2701 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 2761 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 2821 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 2881 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg 2941 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 3001 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 3061 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 3121 tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa 3181 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 3241 caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 3301 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3361 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 3421 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 3481 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 3541 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 3601 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 3661 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 3721 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 3781 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 3841 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 3901 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 3961 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 4021 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 4081 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 4141 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 4201 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 4261 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 4321 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 4381 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 4441 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 4501 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 4561 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4621 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4681 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 4741 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 4801 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 4861 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 4921 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 4981 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 5041 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 5101 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 5161 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 5221 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 5281 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 5341 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 5401 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 5461 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 5521 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 5581 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5641 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 5701 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5761 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 5821 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5881 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5941 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 6001 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 6061 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 6121 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 6181 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 6241 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 6301 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 6361 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 6421 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 6481 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 6541 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 6601 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6661 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6721 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 6781 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6841 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6901 acgtc //