LOCUS M13KE 7222 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector M13KE, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector M13KE ORGANISM Cloning vector M13KE other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7222) AUTHORS Zwick,M.B., Bonnycastle,L.L., Noren,K.A., Venturini,S., Leong,E., Barbas,C.F. III, Noren,C.J. and Scott,J.K. TITLE The maltose-binding protein as a scaffold for monovalent display of peptides derived from phage libraries JOURNAL Anal Biochem 264 (1), 87-97 (1998) PUBMED 9784192 REFERENCE 2 (bases 1 to 7222) AUTHORS Noren,K.A. and Noren,C.J. TITLE Construction of high-complexity combinatorial phage display peptide libraries JOURNAL Methods 23 (2), 169-178 (2001) PUBMED 11181036 REFERENCE 3 (bases 1 to 7222) AUTHORS Stewart,F.J. TITLE Sequence of M13KE JOURNAL Unpublished REFERENCE 4 (bases 1 to 7222) AUTHORS Paschal,B.M. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT Corrects errors in M13KE sequence dated 11 Nov 1997 based on 2002 sequencing of complete M13KE phage genome at NEB (Stewart, F.J., unpublished). FEATURES Location/Qualifiers source 1..7222 /organism="Cloning vector M13KE" /mol_type="other DNA" gene join(6821..7222,1..831) /gene="II" CDS join(6821..7222,1..831) /gene="II" /codon_start=1 /product="pII" /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGINLSA RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI DHLRNVSNGQTKSTRSQNWESTVIWNETSRHRTLVAYLKHVELQHYIQQLSSKPSAKM TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQG KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV DFSSQRPDWYNEPVLKIA" gene 496..831 /gene="X" CDS 496..831 /gene="X" /codon_start=1 /product="pX" /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW YNEPVLKIA" gene 843..1106 /gene="V" CDS 843..1106 /gene="V" /codon_start=1 /product="pV" /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK" gene 1108..1209 /gene="VII" CDS 1108..1209 /gene="VII" /codon_start=1 /product="pVII" /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR" gene 1206..1304 /gene="IX" CDS 1206..1304 /gene="IX" /codon_start=1 /product="pIX" /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS" gene 1301..1522 /gene="VIII" CDS 1301..1522 /gene="VIII" /codon_start=1 /product="pVIII" /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY IGYAWAMVVVIVGATIGIKLFKKFTSKAS" gene 1578..2852 /gene="III" CDS 1578..2852 /gene="III" /exception="alternative start codon" /codon_start=1 /transl_table=11 /product="pIII" /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKSHTENSFTNVWKDDKT LDRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEG GGTKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFR NRQGALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDLFV CEYQGQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDF DYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGAT GDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKI NLFRGVFAFLLYVATFMYVFSTFANILRNKES" gene 2855..3193 /gene="VI" CDS 2855..3193 /gene="VI" /codon_start=1 /product="pVI" /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ KIVSYLDWDK" gene 3195..4241 /gene="I" CDS 3195..4241 /gene="I" /codon_start=1 /product="pI" /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL YSLITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTV SIKKGNSNEIVKCN" gene 3915..4241 /gene="XI" /note="also called I*" CDS 3915..4241 /gene="XI" /note="also called I*" /codon_start=1 /product="pXI" /translation="MKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVVSQT YDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGNSN EIVKCN" gene 4219..5499 /gene="IV" CDS 4219..5499 /gene="IV" /codon_start=1 /product="pIV" /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGE SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYI DELPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS SNVLSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL" rep_origin 5487..5867 /note="M13 origin of replication (- +)" gene 6216..6695 /gene="lacZalpha" CDS 6216..6695 /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSLHACRSSNSLAVVLQRRDWENPGVTQLNRLAAHPPFA SWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQM HGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVPIG" BASE COUNT 1761 a 1523 c 1525 g 2413 t ORIGIN 1 aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttata tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgagctacag cattatattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1201 caaagatgag tgttttagtg tattcttttg cctctttcgt tttaggttgg tgccttcgta 1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1561 ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtg gtacctttct 1621 attctcactc ggccgaaact gttgaaagtt gtttagcaaa atcccataca gaaaattcat 1681 ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggctgtc 1741 tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat 1801 gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt 1861 ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta 1921 ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa 1981 accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc 2041 agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc 2101 aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt 2161 atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgagg 2221 atttatttgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg 2281 ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg 2341 gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg 2401 attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg 2461 aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg 2521 ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg 2581 gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt 2641 taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt 2701 ttgtctttgg cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat 2761 tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt 2821 ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt 2881 attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct 2941 taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg 3001 gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt 3061 tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct 3121 ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga 3181 ttgggataaa taatatggct gtttattttg taactggcaa attaggctct ggaaagacgc 3241 tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc 3301 ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc 3361 ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc ggtaatgatt 3421 cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact tggtttaata 3481 cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta catgctcgta 3541 aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat aaacaggcgc 3601 gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt actttacctt 3661 ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct aaattacatg 3721 ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt tggctttata 3781 ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt aattatgatt 3841 ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc aaaccattaa 3901 atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt 3961 gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa cctaagccgg 4021 aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt gactcttctc 4081 agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata 4141 gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt actgtttcca 4201 ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt 4261 gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct gcgcgatttt 4321 gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga tgtaaaaggt 4381 actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt ctttatttct 4441 gttttacgtg caaataattt tgatatggta ggttctaacc cttccattat tcagaagtat 4501 aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca ggaatatgat 4561 gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaact 4621 tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt gtttgtaaag 4681 tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct attagttgtt 4741 agtgctccta aagatatttt agataacctt cctcaattcc tttcaactgt tgatttgcca 4801 actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga tgctttagat 4861 ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa tactgaccgc 4921 ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg cgatgtttta 4981 gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgccacgt 5041 attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt tccttttatt 5101 actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac gattgagcgt 5161 caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt 5221 ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt 5281 actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac tcttttactc 5341 ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt cctgtctaaa 5401 atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga aagcacgtta 5461 tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 5521 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 5581 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 5641 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 5701 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 5761 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 5821 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa 5881 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 5941 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 6001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 6061 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 6121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 6181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 6241 atgcctgcag gtcctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 6301 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 6361 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 6421 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc 6481 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca 6541 tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc 6601 cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga 6661 cgcgaattat ttttgatggc gttcctattg gttaaaaaat gagctgattt aacaaaaatt 6721 taatgcgaat tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt 6781 cctgtttttg gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt 6841 acgattaccg ttcatcgatt ctcttgtttg ctccagactc tcaggcaatg acctgatagc 6901 ctttgtagat ctctcaaaaa tagctaccct ctccggcatt aatttatcag ctagaacggt 6961 tgaatatcat attgatggtg atttgactgt ctccggcctt tctcaccctt ttgaatcttt 7021 acctacacat tactcaggca ttgcatttaa aatatatgag ggttctaaaa atttttatcc 7081 ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag ggtcataatg tttttggtac 7141 aaccgattta gctttatgct ctgaggcttt attgcttaat tttgctaatt ctttgccttg 7201 cctgtatgat ttattggatg tt //