LOCUS pUC19 2686 bp DNA circular 18-OCT-2007 DEFINITION Cloning vector pUC19, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pUC19 ORGANISM Cloning vector pUC19 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2686) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (18-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank accession L09137. FEATURES Location/Qualifiers source 1..2686 /organism="Cloning vector pUC19" /mol_type="other DNA" gene complement(146..469) /gene="lacZalpha" CDS complement(146..469) /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI CSDAA" misc_feature 396..452 /gene="lacZalpha" /note="multiple cloning site (EcoRI-HindIII)" -10_signal complement(514..519) /note="Plac promoter (drives lacZalpha; counter-clockwise); TATGTT" -35_signal complement(538..543) /note="Plac promoter (drives lacZalpha; counter-clockwise); TTTACA" protein_bind complement(563..575) /note="CAP protein binding site" /bound_moiety="catabolite activator protein (CAP)" rep_origin complement(867..1455) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" misc_RNA complement(867..1419) /note="RNAII transcript (complementary strand)" -35_signal 1273..1278 /note="RNAI promoter (clockwise); TTGAAG" -10_signal 1295..1300 /note="RNAI promoter (clockwise); GCTACA" misc_RNA 1309..1416 /note="RNAI transcript" -10_signal complement(1429..1434) /note="RNAII promoter (counter-clockwise); CGTAAT" -35_signal complement(1450..1455) /note="RNAII promoter (counter-clockwise); TTGAGA" gene complement(1626..2486) /gene="bla" CDS complement(1626..2486) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(2418..2486) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." -10_signal complement(2530..2535) /note="bla promoter (counter-clockwise); GAGACA" -35_signal complement(2551..2556) /note="bla promoter (counter-clockwise); TTCAAA" BASE COUNT 667 a 675 c 685 g 659 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct 481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 1981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 2281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 2521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 2581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 2641 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc //