Carrier Protein 39 (Western) Protocol 1: For Western Blotting

Protocol

  1. Dissolve your peptide in water to a concentration of 1 mM.
  2. Thaw the carrier protein at room temperature.
  3. Mix the following (in order):
  4. Incubate at room temperature for 15-60 minutes or at 4°C overnight.
  5. Add 12.5 µl of 3X SDS-PAGE Sample Buffer.
  6. Heat at 95°C for 5 minutes.
  7. Load 5-10 µl/lane (~ 25-50 ng) for Western blotting.