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Product Information
PstI has a High Fidelity version PstI-HF® (NEB #R3140).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.Product Source
A E. coli strain that carries the PstI gene from Providencia stuartii 164 (ATCC 49762).- This product is related to the following categories:
- Restriction Endonucleases P R Products,
- Time-Saver Qualified Restriction Enzymes Products
- This product can be used in the following applications:
- BioBrick® Assembly,
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Product Citation Tool
Additional Citations
- Yang GQ, Chen YM, Wang JP, Guo C, Zhao L, Wang XY, Guo Y, Li L, Li DZ, Guo ZH. (2016) Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants. Plant Methods; Aug 4;12, 39.
- Guo B, DeFaveri J, Sotelo G, Nair A, Merilä J. (2015) Population genomic evidence for adaptive differentiation in Baltic Sea three-spined sticklebacks. BMC Biol; Mar 24;13:19,
- Combosch DJ, Vollmer SV. (2015) Trans-Pacific RAD-Seq population genomics confirms introgressive hybridization in Eastern Pacific Pocillopora corals. Mol Phylogenet Evol; Jul;88, 154-62.
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Featured Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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TIME-SAVER™ Protocol for Restriction Enzyme Digests
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NEB® TV Ep. 15 – Applications of Restriction Enzymes
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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