Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.
The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535). Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (96 Index Primers)
This product is related to the following categories:
SPRIselect or AMPure® XP Beads (Beckman Coulter, Inc.)
DNA LoBind® Tubes (Eppendorf®)/PCR Plates
Magnetic Stand
Bioanalyzer® (Agilent Technologies, Inc.)
Freshly Prepared 80% Ethanol
Please Refer to the Kit Specific Protocol for using the NEBNext Multiplex Oligos for Illumina: The following kits are designed for use with the NEBNext Multiplex Oligos for Illumina:
#E7760, NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
#E7765, NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads
#E7770, NEBNext Ultra II RNA library Prep Kit for Illumina
#E7775, NEBNext Ultra II RNA Library Prep with Sample Purification Beads
#E7805, NEBNext Ultra II FS DNA Library Prep Kit for Illumina
#E6177, NEBNext Ultra II FS DNA Library Prep Kit with Sample Purification Beads
#E7645, NEBNext Ultra II DNA Library Prep Kit for Illumina
#E7103, NEBNext Ultra II DNA Library Prep with Sample Purification Beads
Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.
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