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- Enzymes powerful enough to digest in 5 minutes
- Pure enough for overnight incubation
- No special formulation
- No added expense
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 Look for this Time-Saver™ icon
on product page. Over 150 of NEB enzymes are Time-Saver qualified and will digest 1 µg
of DNA in 5 minutes. |
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| At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction-modification
systems. This focus allows us to provide extremely pure enzymes at concentrations that deliver more
flexibility to your experimental design. |
| Whether you are quickly screening large numbers of clones or setting up overnight digests,
you will benefit from the high quality of our enzymes. Typically, a restriction digest
involves the incubation of 1 µl of enzyme with 1 µg of purified DNA in a
final volume of 50 µl for 1 hour. However, to speed up the screening process, choose
one of NEB’s enzymes that are Time-Saver qualified. These enzymes will digest 1 µg
of DNA in 5 minutes using 1 µl of enzyme under recommended reaction conditions.
Unlike other suppliers, there is no special formulation, change in concentration or need
to buy more expensive new lines of enzymes to achieve digestion in 5 minutes. In fact,
63% of our enzymes will digest 1 µg of DNA in 5 minutes, while 83% will fully digest
1 µg of DNA in 15 minutes (see table below). That means
over 199 of our restriction enzymes have the power to get the job done fast. |
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Power and purity of Time-Saver qualified
enzymes: 1 µl of SalI digests 1 µg
of DNA in 5 minutes with no indication of nuclease contamination in longer digests or
overnight (O/N) samples. Marker (M) is the 1
kb DNA Ladder (NEB #N3232). |
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In an effort to provide you with as much information as possible, NEB has tested all of its enzymes
on unit assay substrate as well as plasmid substrate. By definition, Time-Saver qualified restriction
enzymes digest unit assay substrate in 5 minutes. The rate of cutting for plasmid substrate is also
given; however, we recommend that this be used as a guide and is not definitive for all plasmids.
Restriction enzymes can often show site preference, presumably determined by the sequence flanking
the recognition site. In addition, supercoiled DNA may have
varying rates of cleavage.
Since all of our enzymes are rigorously tested for nuclease contamination, you can also safely set
up digests for long periods of time without sample degradation. Only NEB can offer enzymes with power
and purity – the power to digest in 5 minutes and the purity to withstand overnight digestions
with no loss of sample. |
Please check out other technical reference information related to restriction enzymes:
NEBuffer Activity | High
Fidelity (HF) Restriction Enzymes | Double Digestion | Heat
Inactivation |
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Recombinant |
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Digest in 5 minutes |
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Enzymes that are Time-Saver qualified will digest unit assay substrate
in 5 minutes under recommended reaction conditions |
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Digest in 15 minutes |
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Not completely digested in 15 minutes |
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Not tested. |
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fast digest, fastdigest, fast digestion, time saver
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