At NEB, enzyme production is linked to basic research in the cloning and overexpression of
restriction-modification systems. This focus allows us to provide extremely pure enzymes at
concentrations that deliver more flexibility for your experimental design.
Whether you are quickly screening large numbers of clones, or setting up overnight digests, you will benefit from the high quality of our enzymes. Typically, a restriction digest involves the incubation of 1 µl of enzyme with 1 µg of purified DNA in a final volume of 50 µl for 1 hour. However, to speed up the screening process, choose one of NEB’s enzymes that are Time-Saver Qualified. These enzymes will digest 1 µg of DNA in 5 minutes using 1 µl of enzyme under recommended reaction conditions. Unlike other suppliers, there is no special formulation, change in concentration or need to buy more expensive new lines of enzymes to achieve digestion in 5 minutes. In fact, 63% of our enzymes will digest 1 µg of DNA in 5 minutes, while 83% will fully digest in 15 minutes (see table). That means >200 of our restriction enzymes have the power to get the job done fast.
In an effort to provide you with as much information as possible, NEB has tested all of its enzymes on unit assay substrate as well as plasmid substrate. By definition, Time-Saver qualified restriction enzymes digest unit assay substrate in 5 minutes. The rate of cutting for plasmid substrate is also given; however, we recommend that this be used as a guide and is not definitive for all plasmids. Restriction enzymes can often show site preference, presumably determined by the sequence flanking the recognition site. In addition, supercoiled DNA may have varying rates of cleavage.
Also, since all of our enzymes are rigorously tested for nuclease contamination, you can safely set up digests for long periods of time without any degradation of your sample. Only NEB can offer you enzymes with power and purity — the power to digest in 5 minutes and the purity to withstand
overnight digestions with no loss of sample.
|
