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Home >  Technical Reference > Restriction Endonucleases > Reduced Star Activities of HF Enzymes
Reduced Star Activities of HF Enzymes

The following table indicates the number of units of HF-enzyme that can be used compared to the wild type counterpart before any significant star activity is detected. The HF Factor refers to the X-fold increase that is achieved by choosing an HF enzyme. This data clearly illustrates the flexibility that is offered by using an HF restriction enzyme.

Enzyme Buffer1 Max. Units with no Star Activity2 HF Factor
AgeI-HF™ NEBuffer 4 + BSA 250 8
AgeI NEBuffer 1 32  
BamHI-HF™ NEBuffer 4 4,000 125
BamHI NEBuffer 3 + BSA 32  
BsaI-HF™ NEBuffer 4 + BSA 8,000 250
BsaI NEBuffer 4 + BSA 32  
EagI-HF™ NEBuffer 4 500 2
EagI NEBuffer 3 250  
EcoRI-HF™ NEBuffer 4 16,000 64
EcoRI NEBuffer EcoRI 250  
EcoRV-HF™ NEBuffer 4 64,000 64
EcoRV NEBuffer 3 + BSA 1,000  
KpnI-HF™ NEBuffer 4 250 16
KpnI NEBuffer 1 + BSA 16  
MfeI-HF™ NEBuffer 4 500 15
MfeI NEBuffer 4 32  
NcoI-HF™ NEBuffer 4 16,000 133
NcoI NEBuffer 3 120  
NheI-HF™ NEBuffer 4 + BSA 32,000 266
NheI NEBuffer 2 + BSA 120  
NotI-HF™ NEBuffer 4 + BSA 64,000 16
NotI NEBuffer 3 + BSA 4,000  
PstI-HF™ NEBuffer 4 4,000 33
PstI NEBuffer 3+ BSA 120  
PvuII-HF™ NEBuffer 4 500 31
PvuII NEBuffer 2 16  
SacI-HF™ NEBuffer 4 + BSA 4,000 33
SacI NEBuffer 1 + BSA 120  
SalI-HF™ NEBuffer 4 2,000 500
SalI NEBuffer 3 + BSA 4  
SbfI-HF™ NEBuffer 4 250 31
SbfI NEBuffer 4 8  
ScaI-HF™ NEBuffer 4 250 62
ScaI NEBuffer 3 4  
SphI-HF™ NEBuffer 4 2,000 62
SphI NEBuffer 2 32  
SspI-HF™ NEBuffer 4 500  
SspI NEBuffer SspI ND3  
StyI-HF™ NEBuffer 4 4,000 125
StyI NEBuffer 3 + BSA 32  
  1. Wild type enzymes were tested in supplied buffer for comparisons.
  2. Wei, H. et al (2008) Nucleic Acids Reseach 36, e50.
  3. Not determined.

Please check out other technical reference information related to restriction enzymes:
NEBuffer Activity | Star Activity | High Fidelity (HF) Restriction Enzymes  | Double Digestion | Time-Saver Enzymes

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