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Home >  Technical Reference > Restriction Endonucleases > Nonpalindromic Sequences Enzymes with Nonpalindromic Sequences Single Letter Code Enzyme Recognition Sequence AciI CCGC(-3/-1) AcuI CTGAAG(16/14) AlwI GGATC(4/5) BaeI (10/15)ACNNNNGTAYC(12/7) BbsI GAAGAC(2/6) BbvCI CCTCAGC(-5/-2) BbvI GCAGC(8/12) BccI CCATC(4/5) BceAI ACGGC(12/14) BcgI (10/12)CGANNNNNNTGC(12/10) BciVI GTATCC(6/5) BfuAI ACCTGC(4/8) BmgBI CACGTC(-3/-3) BmrI ACTGGG(5/4) BpmI CTGGAG(16/14) Bpu10I CCTNAGC(-5/-2) BpuEI CTTGAG(16/14) BsaI GGTCTC(1/5) BsaI-HF™ GGTCTC(1/5) BsaI-HF™ GGTCTC(1/5) BsaXI (9/12)ACNNNNNCTCC(10/7) BseRI GAGGAG(10/8) BseYI CCCAGC(-5/-1) BsgI GTGCAG(16/14) BsmAI GTCTC(1/5) BsmBI CGTCTC(1/5) BsmFI GGGAC(10/14) BsmI GAATGC(1/-1) BspCNI CTCAG(9/7) BspMI ACCTGC(4/8) BspQI GCTCTTC(1/4) BsrBI CCGCTC(-3/-3) BsrDI GCAATG(2/0) BsrI ACTGG(1/-1) BssSI CACGAG(-5/-1) BtgZI GCGATG(10/14) BtsCI GGATG(2/0) BtsI GCAGTG(2/0) CspCI (11/13)CAANNNNNGTGG(12/10) EarI CTCTTC(1/4) EciI GGCGGA(11/9) EcoP15I CAGCAG(25/27) FauI CCCGC(4/6) FokI GGATG(9/13) HgaI GACGC(5/10) HphI GGTGA(8/7) HpyAV CCTTC(6/5) I-CeuI CGTAACTATAACGGTCCTAAGGTAGCGAA(-9/-13) I-SceI TAGGGATAACAGGGTAAT(-9/-13) MboII GAAGA(8/7) MlyI GAGTC(5/5) MmeI TCCRAC(20/18) MnlI CCTC(7/6) Nb.BbvCI CCTCAGC Nb.BbvCI CCTCAGC Nb.BsmI GAATGC Nb.BsmI GAATGC Nb.BsrDI GCAATG Nb.BsrDI GCAATG Nb.BtsI GCAGTG Nb.BtsI GCAGTG NmeAIII GCCGAG(21/19) Nt.AlwI GGATC(4/-5) Nt.AlwI GGATC(4/-5) Nt.BbvCI CCTCAGC(-5/-7) Nt.BbvCI CCTCAGC(-5/-7) Nt.BsmAI GTCTC(1/-5) Nt.BsmAI GTCTC(1/-5) Nt.BspQI GCTCTTC(1/-7) Nt.BspQI GCTCTTC(1/-7) Nt.BstNBI GAGTC(4/-5) Nt.BstNBI GAGTC(4/-5) Nt.CviPII (0/-1)CCD Nt.CviPII (0/-1)CCD PI-PspI TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17) PI-SceI ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19) PleI GAGTC(4/5) SapI GCTCTTC(1/4) SfaNI GCATC(5/9) New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201