|
Restriction endonucleases that recognize the same sequence are isoschizomers. The list below contains isoschizomers
for commercially available restriction endonucleases. It also specifies which isoschizomer is available from New
England Biolabs. Additional prototypes that are not commercially available are also listed.
All recognition sequences are written 5´ to 3´ using the single
letter code nomenclature with the point of cleavage indicated by a "/". Numbers in parentheses indicate point
of cleavage for non-palindromic enzymes. For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´
Isoschizomers with alternative cleavage sites are indicated with a " ^ ". Enzymes that
are not currently commercially available are indicated with a " x ".
| A | B | C | D | E | F | G | H | I | K | L | M | N | O | P | R | S | T | U | V | X | Z |
| Enzyme |
Sequence |
NEB Enzyme |
Catalog # |
Sequence |
OtherIsoschizomers |
| AarI |
CACCTGC (4/8) |
|
|
|
|
| AasI |
GACNNNN/NNGTC |
DrdI |
R0530 |
GACNNNN/NNGTC |
DrdI, DseDI |
| AatI |
AGG/CCT |
StuI |
R0187 |
AGG/CCT |
Eco147I, PceI, SseBI, StuI |
| AatII |
GACGT/C |
AatII |
R0117 |
GACGT/C |
ZraI^ |
| |
|
ZraI^ |
R0659 |
GAC/GTC |
|
| AccI |
GT/MKAC |
AccI |
R0161 |
GT/MKAC |
FblI, XmiI |
| AccII |
CG/CG |
BstUI |
R0518 |
CG/CG |
Bsh1236I, BstFNI, BstUI, MvnI |
| AccIII |
T/CCGGA |
BspEI |
R0540 |
T/CCGGA |
BseAI, Bsp13I, BspEI, Kpn2I, MroI |
| Acc16I |
TGC/GCA |
FspI |
R0135 |
TGC/GCA |
AviII, FspI, NsbI |
| Acc36I |
ACCTGC (4/8) |
BfuAI |
R0701 |
ACCTGC (4/8) |
BfuAI, BspMI, BveI |
| |
|
BspMI |
R0502 |
ACCTGC (4/8) |
|
| Acc65I |
G/GTACC |
Acc65I |
R0599 |
G/GTACC |
Asp718I, KpnI^ |
| |
|
KpnI^ |
R0142 |
GGTAC/C |
|
| AccB1I |
G/GYRCC |
BanI |
R0118 |
G/GYRCC |
BanI, BshNI, BspT107I |
| AccB7I |
CCANNNN/NTGG |
PflMI |
R0509 |
CCANNNN/NTGG |
PflBI, PflMI,Van91I |
| AccBSI |
CCGCTC (-3/-3) |
BsrBI |
R0102 |
CCGCTC (-3/-3) |
BsrBI, MbiI |
| AceIIIx |
CAGCTC (7/11) |
|
|
|
|
| AciI |
CCGC (-3/-1) |
AciI |
R0551 |
CCGC (-3/-1) |
SsiI |
| AclI |
AA/CGTT |
AclI |
R0598 |
AA/CGTT |
Psp1406I |
| AclWI |
GGATC (4/5) |
AlwI |
R0513 |
GGATC (4/5) |
AlwI, BspPI |
| AcsI |
R/AATTY |
ApoI |
R0566 |
R/AATTY |
ApoI, XapI |
| AcuI |
CTGAAG (16/14) |
AcuI |
R0641 |
CTGAAG (16/14) |
Eco57I |
| AcvI |
CAC/GTG |
PmlI |
R0532 |
CAC/GTG |
BbrPI, Eco72I, PmaCI, PmlI, PspCI |
| AcyI |
GR/CGYC |
BsaHI |
R0556 |
GR/CGYC |
BsaHI, BstACI, Hin1I, Hsp92I |
| AdeI |
CACNNN/GTG |
DraIII |
R0510 |
CACNNN/GTG |
DraIII |
| AfaI |
GT/AC |
RsaI |
R0167 |
GT/AC |
Csp6I^, RsaI |
| AfeI |
AGC/GCT |
AfeI |
R0652 |
AGC/GCT |
Aor51HI, Eco47III, FunI |
| AflII |
C/TTAAG |
AflII |
R0520 |
C/TTAAG |
BfrI, BspTI, Bst98I, MspCI,Vha464I |
| AflIII |
A/CRYGT |
AflIII |
R0541 |
A/CRYGT |
|
| AgeI |
A/CCGGT |
AgeI |
R0552 |
A/CCGGT |
AsiAI, BshTI, CspAI, PinAI |
| AhaIIIx |
TTT/AAA |
DraI |
R0129 |
TTT/AAA |
DraI |
| AhdI |
GACNNN/NNGTC |
AhdI |
R0584 |
GACNNN/NNGTC |
AspEI, DriI, Eam1105I, EclHKI |
| AhlI |
A/CTAGT |
SpeI |
R0133 |
A/CTAGT |
BcuI, SpeI |
| AjnI |
/CCWGG |
BstNI^ |
R0168 |
CC/WGG |
BseBI^, BstNI^, BstOI^,
Bst2UI^, EcoRII, MvaI^, Psp6I, PspGI |
| |
|
PspGI |
R0611 |
/CCWGG |
|
| AleI |
CACNN/NNGTG |
AleI |
R0734 |
CACNN/NNGTG |
OliI |
| AlfI |
(10/12)GCANNNNNNTGC(12/10) |
|
|
|
|
| AloI |
(7/12) GAACNNNNNNTCC (12/7) |
|
|
|
|
| AluI |
AG/CT |
AluI |
R0137 |
AG/CT |
|
| AlwI |
GGATC (4/5) |
AlwI |
R0513 |
GGATC (4/5) |
AclWI, BspPI |
| Alw21I |
GWGCW/C |
BsiHKAI |
R0570 |
GWGCW/C |
AspHI, Bbv12I, BsiHKAI |
| Alw26I |
GTCTC (1/5) |
BsmAI |
R0529 |
GTCTC (1/5) |
BsmAI, BsoMAI |
| Alw44I |
G/TGCAC |
ApaLI |
R0507 |
G/TGCAC |
ApaLI,VneI |
| AlwNI |
CAGNNN/CTG |
AlwNI |
R0514 |
CAGNNN/CTG |
CaiI |
| Ama87I |
C/YCGRG |
AvaI |
R0152 |
C/YCGRG |
AvaI, BsiHKCI, BsoBI, Eco88I, NspIII |
| |
|
BsoBI |
R0586 |
C/YCGRG |
|
| Aor51HI |
AGC/GCT |
AfeI |
R0652 |
AGC/GCT |
AfeI, Eco47III, FunI |
| ApaI |
GGGCC/C |
ApaI |
R0114 |
GGGCC/C |
Bsp120I^, PspOMI^ |
| |
|
PspOMI^ |
V0215 |
G/GGCCC |
|
| ApaBIx |
GCANNNNN/TGC |
BstAPI^ |
V0259 |
GCANNNN/NTGC |
BstAPI^ |
| ApaLI |
G/TGCAC |
ApaLI |
R0507 |
G/TGCAC |
Alw44I,VneI |
| ApeKI |
G/CWGC |
ApeKI |
R0643 |
G/CWGC |
TseI |
| |
|
TseI |
R0591 |
G/CWGC |
|
| ApoI |
R/AATTY |
ApoI |
R0566 |
R/AATTY |
AcsI, XapI |
| AscI |
GG/CGCGCC |
AscI |
R0558 |
GG/CGCGCC |
|
| AseI |
AT/TAAT |
AseI |
R0526 |
AT/TAAT |
PshBI,VspI |
| AsiAI |
A/CCGGT |
AgeI |
R0552 |
A/CCGGT |
AgeI, BshTI, CspAI, PinAI |
| AsiSI |
GCGAT/CGC |
AsiSI |
R0630 |
GCGAT/CGC |
SgfI |
| AspI |
GACN/NNGTC |
PflFI |
R0595 |
GACN/NNGTC |
PflFI, PsyI, TelI, Tth111I |
| |
|
Tth111I |
R0185 |
GACN/NNGTC |
|
| Asp700I |
GAANN/NNTTC |
XmnI |
R0194 |
GAANN/NNTTC |
MroXI, PdmI, XmnI |
| Asp718I |
G/GTACC |
Acc65I |
R0599 |
G/GTACC |
Acc65I, KpnI^ |
| |
|
KpnI^ |
R0142 |
GGTAC/C |
|
| AspA2I |
C/CTAGG |
AvrII |
R0174 |
C/CTAGG |
AvrII, BlnI, XmaJI |
| AspEI |
GACNNN/NNGTC |
AhdI |
R0584 |
GACNNN/NNGTC |
AhdI, DriI, Eam1105I, EclHKI |
| AspHI |
GWGCW/C |
BsiHKAI |
R0570 |
GWGCW/C |
Alw21I, Bbv12I, BsiHKAI |
| AspLEI |
GCG/C |
HhaI |
R0139 |
GCG/C |
BstHHI, CfoI, HhaI, Hin6I^, HinP1I^,
HspAI^ |
| |
|
HinP1I^ |
R0124 |
G/CGC |
|
| AspS9I |
G/GNCC |
Sau96I |
R0165 |
G/GNCC |
Cfr13I, PspPI, Sau96I |
| AsuIx |
G/GNCC |
Sau96I |
R0165 |
G/GNCC |
AspS9I, Cfr13I, PspPI, Sau96I |
| AsuII |
TT/CGAA |
BstBI |
R0519 |
TT/CGAA |
Bpu14I, Bsp119I, BspT104I, BstBI, Csp45I, NspV,SfuI |
| AsuC2I |
CC/SGG |
NciI |
R0196 |
CC/SGG |
BcnI, NciI |
| AsuHPI |
GGTGA (8/7) |
HphI |
R0158 |
GGTGA (8/7) |
HphI |
| AsuNHI |
G/CTAGC |
BmtI^ |
R0658 |
GCTAG/C |
NheI, BmtI^ |
| |
|
NheI |
R0131 |
G/CTAGC |
|
| AvaI |
C/YCGRG |
AvaI |
R0152 |
C/YCGRG |
Ama87I, BsiHKCI, BsoBI, Eco88I, NspIII |
| |
|
BsoBI |
R0586 |
C/YCGRG |
|
| AvaII |
G/GWCC |
AvaII |
R0153 |
G/GWCC |
Bme18I, Eco47I, SinI,VpaK11BI |
| AvaIIIx |
ATGCAT |
NsiI^ |
R0127 |
ATGCA/T |
BfrBI, EcoT22I^, Mph1103I^, NsiI^,
Zsp2I^ |
| AviII |
TGC/GCA |
FspI |
R0135 |
TGC/GCA |
Acc16I, FspI, NsbI |
| AvrII |
C/CTAGG |
AvrII |
R0174 |
C/CTAGG |
AspA2I, BlnI, XmaJI |
| AxyI |
CC/TNAGG |
Bsu36I |
R0524 |
CC/TNAGG |
Bse21I, Bsu36I, Eco81I |
| top |
| BaeI |
(10/15) ACNNNNGTAYC (12/7) |
BaeI |
R0613 |
(10/15) ACNNNNGTAYC (12/7) |
|
| BaeGI |
GKGCM/C |
BaeGI |
R0708 |
GKGCM/C |
Bme1580I, BseSI |
| |
|
Bme1580I |
|
GKGCM/C |
|
| BalI |
TGG/CCA |
MscI |
R0534 |
TGG/CCA |
MlsI, MluNI, MscI, Msp20I |
| BamHI |
G/GATCC |
BamHI |
R0136 |
G/GATCC |
|
| BanI |
G/GYRCC |
BanI |
R0118 |
G/GYRCC |
AccB1I, BshNI, BspT107I |
| BanII |
GRGCY/C |
BanII |
R0119 |
GRGCY/C |
Eco24I, EcoT38I, FriOI |
| BanIII |
AT/CGAT |
BspDI |
R0557 |
AT/CGAT |
Bsa29I, BseCI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI |
| |
|
ClaI |
R0197 |
AT/CGAT |
|
| BbeI |
GGCGC/C |
KasI^ |
R0544 |
G/GCGCC |
EgeI^^, EheI^^,
KasI^, Mly113I^^^,
NarI^^^, SfoI^^ |
| |
|
NarI^^^ |
R0191 |
GG/CGCC |
|
| |
|
SfoI^^ |
R0606 |
GGC/GCC |
|
| BbrPI |
CAC/GTG |
PmlI |
R0532 |
CAC/GTG |
AcvI, Eco72I, PmaCI, PmlI |
| BbsI |
GAAGAC (2/6) |
BbsI |
R0539 |
GAAGAC (2/6) |
BpiI, BpuAI, BstV2I |
| BbuI |
GCATG/C |
SphI |
R0182 |
GCATG/C |
PaeI, SpaHI, SphI |
| BbvI |
GCAGC (8/12) |
BbvI |
R0173 |
GCAGC (8/12) |
BseXI, BstV1I |
| BbvIIx |
GAAGAC (2/6) |
BbsI |
R0539 |
GAAGAC (2/6) |
BbsI, BpiI, BpuAI, BstV2I |
| Bbv12I |
GWGCW/C |
BsiHKAI |
R0570 |
GWGCW/C |
Alw21I, AspHI, BsiHKAI |
| BbvCI |
CCTCAGC (-5/-2) |
BbvCI |
R0601 |
CCTCAGC (-5/-2) |
|
| BccI |
CCATC(4/5) |
BccI |
R0704 |
CCATC(4/5) |
|
| Bce83Ix |
CTTGAG (16/14) |
BpuEI |
R0633 |
CTTGAG (16/14) |
BpuEI |
| BceAI |
ACGGC (12/14) |
BceAI |
R0623 |
ACGGC (12/14) |
|
| BcefIx |
ACGGC (12/13) |
BceAI^ |
R0623 |
ACGGC (12/14) |
BceAI^ |
| BcgI |
(10/12) CGANNNNNNTGC (12/10) |
BcgI |
R0545 |
(10/12) CGANNNNNNTGC (12/10) |
|
| BciVI |
GTATCC (6/5) |
BciVI |
R0596 |
GTATCC (6/5) |
BfuI |
| BclI |
T/GATCA |
BclI |
R0160 |
T/GATCA |
FbaI, Ksp22I |
| BcnI |
CC/SGG |
NciI |
R0196 |
CC/SGG |
AsuC2I, NciI |
| BcuI |
A/CTAGT |
SpeI |
R0133 |
A/CTAGT |
AhlI, SpeI |
| BetIx |
W/CCGGW |
BsaWI |
R0567 |
W/CCGGW |
BsaWI |
| BfaI |
C/TAG |
BfaI |
R0568 |
C/TAG |
FspBI, MaeI, XspI |
| BfiI |
ACTGGG (5/4) |
BmrI |
R0600 |
ACTGGG (5/4) |
BmrI |
| BfmI |
C/TRYAG |
SfcI |
R0561 |
C/TRYAG |
BstSFI, SfcI |
| BfrI |
C/TTAAG |
AflII |
R0520 |
C/TTAAG |
AflII, BspTI, Bst98I, MspCI,Vha464I |
| BfrBIx |
ATGCA/T |
NsiI^ |
R0127 |
ATGCA/T |
EcoT22I^, Mph1103I^, NsiI^,
Zsp2I^ |
| BfuI |
GTATCC (6/5) |
BciVI |
R0596 |
GTATCC (6/5) |
BciVI |
| BfuAI |
ACCTGC (4/8) |
BfuAI |
R0701 |
ACCTGC (4/8) |
Acc36I, BspMI, BveI |
| |
|
BspMI |
R0502 |
ACCTGC (4/8) |
|
| BfuCI |
/GATC |
BfuCI |
|
/GATC |
Bsp143I, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI |
| |
|
DpnII |
R0543 |
/GATC |
|
| |
|
MboI |
R0147 |
/GATC |
|
| |
|
Sau3AI |
R0169 |
/GATC |
|
| BglI |
GCCNNNN/NGGC |
BglI |
R0143 |
GCCNNNN/NGGC |
|
| BglII |
A/GATCT |
BglII |
R0144 |
A/GATCT |
|
| BinIx |
GGATC (4/5) |
AlwI |
R0513 |
GGATC (4/5) |
AclWI, AlwI, BspPI |
| BlnI |
C/CTAGG |
AvrII |
R0174 |
C/CTAGG |
AspA2I, AvrII, XmaJI |
| BlpI |
GC/TNAGC |
BlpI |
R0585 |
GC/TNAGC |
Bpu1102I, Bsp1720I, CelII |
| Bme18I |
G/GWCC |
AvaII |
R0153 |
G/GWCC |
AvaII, Eco47I, SinI,VpaK11BI |
| Bme1390I |
CC/NGG |
BssKI^ |
R0592 |
/CCNGG |
BssKI^, BstSCI^, MspR9I, ScrFI,
StyD4I^ |
| |
|
ScrFI |
R0110 |
CC/NGG |
|
| |
|
StyD4I^ |
R0638 |
/CCNGG |
|
| Bme1580I |
GKGCM/C |
Bme1580I |
|
GKGCM/C |
BaeGI, BseSI |
| |
|
BaeGI |
R0708 |
GKGCM/C |
|
| BmgIx |
GKGCCC |
|
|
|
|
| BmgBI |
CACGTC (-3/-3) |
BmgBI |
R0628 |
CACGTC (-3/-3) |
BtrI |
| BmrI |
ACTGGG (5/4) |
BmrI |
R0600 |
ACTGGG (5/4) |
BfiI |
| BmtI |
GCTAG/C |
BmtI |
R0658 |
GCTAG/C |
AsuNHI^, NheI^ |
| |
|
NheI^ |
R0131 |
G/CTAGC |
|
| BmyI |
GDGCH/C |
Bsp1286I |
R0120 |
GDGCH/C |
Bsp1286I, MhlI, SduI |
| BoxI |
GACNN/NNGTC |
PshAI |
R0593 |
GACNN/NNGTC |
BstPAI, PshAI |
| BpiI |
GAAGAC (2/6) |
BbsI |
R0539 |
GAAGAC (2/6) |
BbsI, BpuAI, BstV2I |
| BplI |
(8/13) GAGNNNNNCTC (13/8) |
|
|
|
|
| BpmI |
CTGGAG (16/14) |
BpmI |
R0565 |
CTGGAG (16/14) |
GsuI |
| Bpu10I |
CCTNAGC (-5/-2) |
Bpu10I |
V0149 |
CCTNAGC (-5/-2) |
|
| Bpu14I |
TT/CGAA |
BstBI |
R0519 |
TT/CGAA |
AsuII, Bsp119I, BspT104I, BstBI, Csp45I, NspV,SfuI |
| Bpu1102I |
GC/TNAGC |
BlpI |
R0585 |
GC/TNAGC |
BlpI, Bsp1720I, CelII |
| BpuAI |
GAAGAC (2/6) |
BbsI |
R0539 |
GAAGAC (2/6) |
BbsI, BpiI, BstV2I |
| BpuEI |
CTTGAG (16/14) |
BpuEI |
R0633 |
CTTGAG (16/14) |
|
| BsaI |
GGTCTC (1/5) |
BsaI |
R0535 |
GGTCTC (1/5) |
Bso31I, BspTNI, Eco31I |
| Bsa29I |
AT/CGAT |
BspDI |
R0557 |
AT/CGAT |
BanIII, BseCI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI |
| |
|
ClaI |
R0197 |
AT/CGAT |
|
| BsaAI |
YAC/GTR |
BsaAI |
R0531 |
YAC/GTR |
BstBAI |
| BsaBI |
GATNN/NNATC |
BsaBI |
R0537 |
GATNN/NNATC |
Bse8I, BseJI, MamI |
| BsaHI |
GR/CGYC |
BsaHI |
R0556 |
GR/CGYC |
AcyI, BstACI, Hin1I, Hsp92I |
| BsaJI |
C/CNNGG |
BsaJI |
R0536 |
C/CNNGG |
BseDI, BssECI |
| BsaMI |
GAATGC (1/-1) |
BsmI |
R0134 |
GAATGC (1/-1) |
BsmI, Mva1269I, PctI |
| BsaWI |
W/CCGGW |
BsaWI |
R0567 |
W/CCGGW |
|
| BsaXI |
(9/12) ACNNNNNCTCC (10/7) |
BsaXI |
R0609 |
(9/12) ACNNNNNCTCC (10/7) |
|
| BsbIx |
CAACAC |
|
|
|
|
| Bsc4I |
CCNNNNN/NNGG |
BslI |
R0555 |
CCNNNNN/NNGG |
BseLI, BsiYI, BslI |
| BscGIx |
CCCGT |
|
|
|
|
| Bse1I |
ACTGG (1/-1) |
BsrI |
R0527 |
ACTGG (1/-1) |
BseNI, BsrI, BsrSI |
| Bse8I |
GATNN/NNATC |
BsaBI |
R0537 |
GATNN/NNATC |
BsaBI, BseJI, MamI |
| Bse21I |
CC/TNAGG |
Bsu36I |
R0524 |
CC/TNAGG |
AxyI, Bsu36I, Eco81I |
| Bse118I |
R/CCGGY |
BsrFI |
R0562 |
R/CCGGY |
BsrFI, BssAI, Cfr10I |
| BseAI |
T/CCGGA |
BspEI |
R0540 |
T/CCGGA |
AccIII, Bsp13I, BspEI, Kpn2I, MroI |
| BseBI |
CC/WGG |
BstNI |
R0168 |
CC/WGG |
AjnI^, BstNI, BstOI, Bst2UI, EcoRII^,
MvaI, Psp6I^, PspGI^ |
| |
|
PspGI^ |
R0611 |
/CCWGG |
|
| BseCI |
AT/CGAT |
BspDI |
R0557 |
AT/CGAT |
BanIII, Bsa29I, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI |
| |
|
ClaI |
R0197 |
AT/CGAT |
|
| BseDI |
C/CNNGG |
BsaJI |
R0536 |
C/CNNGG |
BsaJI, BssECI |
| Bse3DI |
GCAATG (2/0) |
BsrDI |
R0574 |
GCAATG (2/0) |
BseMI, BsrDI |
| BseGI |
GGATG (2/0) |
BtsCI |
R0647 |
GGATG (2/0) |
BstF5I, FokI^, BtsCI |
| |
|
BstF5I |
|
GGATG (2/0) |
|
| |
|
FokI^ |
R0109 |
GGATG (9/13) |
|
| BseJI |
GATNN/NNATC |
BsaBI |
R0537 |
GATNN/NNATC |
BsaBI, Bse8I, MamI |
| BseLI |
CCNNNNN/NNGG |
BslI |
R0555 |
CCNNNNN/NNGG |
Bsc4I, BsiYI, BslI |
| BseMI |
GCAATG (2/0) |
BsrDI |
R0574 |
GCAATG (2/0) |
Bse3DI, BsrDI |
| BseMII |
CTCAG (10/8) |
BspCNI^ |
R0624 |
CTCAG (9/7) |
BspCNI^ |
| BseNI |
ACTGG (1/-1) |
BsrI |
R0527 |
ACTGG (1/-1) |
Bse1I, BsrI, BsrSI |
| BsePI |
G/CGCGC |
BssHII |
R0199 |
G/CGCGC |
BssHII, PauI |
| BseRI |
GAGGAG (10/8) |
BseRI |
R0581 |
GAGGAG (10/8) |
|
| BseSI |
GKGCM/C |
BaeGI |
R0708 |
GKGCM/C |
BaeGI, Bme1580I |
| |
|
Bme1580I |
|
GKGCM/C |
|
| BseXI |
GCAGC (8/12) |
BbvI |
R0173 |
GCAGC (8/12) |
BbvI, BstV1I |
| BseX3I |
C/GGCCG |
EagI |
R0505 |
C/GGCCG |
BstZI, EagI, EclXI, Eco52I |
| BseYI |
CCCAGC (-5/-1) |
BseYI |
R0635 |
CCCAGC (-5/-1) |
|
| BsgI |
GTGCAG (16/14) |
BsgI |
R0559 |
GTGCAG (16/14) |
|
| Bsh1236I |
CG/CG |
BstUI |
R0518 |
CG/CG |
AccII, BstFNI, BstUI, MvnI |
| Bsh1285I |
CGRY/CG |
BsiEI |
R0554 |
CGRY/CG |
BsiEI, BstMCI |
| BshFI |
GG/CC |
HaeIII |
R0108 |
GG/CC |
BspANI, BsuRI, HaeIII, PalI, PhoI |
| |
|
PhoI |
R0705 |
GG/CC |
|
| BshNI |
G/GYRCC |
BanI |
R0118 |
G/GYRCC |
AccB1I, BanI, BspT107I |
| BshTI |
A/CCGGT |
AgeI |
R0552 |
A/CCGGT |
AgeI, AsiAI, CspAI, PinAI |
| BsiIx |
CACGAG (-5/-1) |
BssSI |
R0587 |
CACGAG (-5/-1) |
BssSI, Bst2BI |
| BsiEI |
CGRY/CG |
BsiEI |
R0554 |
CGRY/CG |
Bsh1285I, BstMCI |
| BsiHKAI |
GWGCW/C |
BsiHKAI |
R0570 |
GWGCW/C |
Alw21I, AspHI, Bbv12I |
| BsiHKCI |
C/YCGRG |
AvaI |
R0152 |
C/YCGRG |
Ama87I, AvaI, BsoBI, Eco88I, NspIII |
| |
|
BsoBI |
R0586 |
C/YCGRG |
|
| BsiSI |
C/CGG |
HpaII |
R0171 |
C/CGG |
HapII, HpaII, MspI |
| |
|
MspI |
R0106 |
C/CGG |
|
| BsiWI |
C/GTACG |
BsiWI |
R0553 |
C/GTACG |
Pfl23II, PspLI, SunI |
| |
|
ClaI |
R0197 |
AT/CGAT |
|
| BsiYI |
CCNNNNN/NNGG |
BslI |
R0555 |
CCNNNNN/NNGG |
Bsc4I, BseLI, BslI |
| BslI |
CCNNNNN/NNGG |
BslI |
R0555 |
CCNNNNN/NNGG |
Bsc4I, BseLI, BsiYI |
| BslFI |
(11/15)GGGAC(10/14) |
BsmFI |
R0572 |
GGGAC(10/14) |
BsmFI |
| BsmI |
GAATGC (1/-1) |
BsmI |
R0134 |
GAATGC (1/-1) |
BsaMI, Mva1269I, PctI |
| BsmAI |
GTCTC (1/5) |
BsmAI |
R0529 |
GTCTC (1/5) |
Alw26I, BsoMAI |
| BsmBI |
CGTCTC (1/5) |
BsmBI |
R0580 |
CGTCTC (1/5) |
Esp3I |
| BsmFI |
GGGAC (10/14) |
BsmFI |
R0572 |
GGGAC (10/14) |
BslFI |
| Bso31I |
GGTCTC (1/5) |
BsaI |
R0535 |
GGTCTC (1/5) |
BsaI, BspTNI, Eco31I |
| BsoBI |
C/YCGRG |
AvaI |
R0152 |
C/YCGRG |
Ama87I, AvaI, BsiHKCI, Eco88I, NspIII |
| |
|
BsoBI |
R0586 |
C/YCGRG |
|
| BsoMAI |
GTCTC (1/5) |
BsmAI |
R0529 |
GTCTC (1/5) |
Alw26I, BsmAI |
| Bsp13I |
T/CCGGA |
BspEI |
R0540 |
T/CCGGA |
AccIII, BseAI, BspEI, Kpn2I, MroI |
| Bsp19I |
C/CATGG |
NcoI |
R0193 |
C/CATGG |
NcoI |
| Bsp24Ix |
(8/13) GACNNNNNNTGG (12/7) |
|
|
|
|
| Bsp68I |
TCG/CGA |
NruI |
R0192 |
TCG/CGA |
NruI |
| Bsp106I |
AT/CGAT |
BspDI |
R0557 |
AT/CGAT |
BanIII, Bsa29I, BseCI, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI |
| |
|
ClaI |
R0197 |
AT/CGAT |
|
| Bsp119I |
TT/CGAA |
BstBI |
R0519 |
TT/CGAA |
AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV,SfuI |
| Bsp120I |
G/GGCCC |
ApaI^ |
R0114 |
GGGCC/C |
ApaI^, PspOMI |
| |
|
PspOMI |
V0215 |
G/GGCCC |
|
| Bsp143I |
/GATC |
BfuCI |
|
/GATC |
BfuCI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI |
| |
|
DpnII |
R0543 |
/GATC |
|
| |
|
MboI |
R0147 |
/GATC |
|
| |
|
Sau3AI |
R0169 |
/GATC |
|
| Bsp143II |
RGCGC/Y |
HaeII |
R0107 |
RGCGC/Y |
BstH2I, HaeII |
| Bsp1286I |
GDGCH/C |
Bsp1286I |
R0120 |
GDGCH/C |
BmyI, MhlI, SduI |
| Bsp1407I |
T/GTACA |
BsrGI |
R0575 |
T/GTACA |
BsrGI, BstAUI, SspBI |
| Bsp1720I |
GC/TNAGC |
BlpI |
R0585 |
GC/TNAGC |
BlpI, Bpu1102I, CelII |
| BspANI |
GG/CC |
HaeIII |
R0108 |
GG/CC |
BshFI, BsuRI, HaeIII, PalI, PhoI |
| |
|
PhoI |
R0705 |
GG/CC |
|
| BspCI |
CGAT/CG |
PvuI |
R0150 |
CGAT/CG |
Ple19I, PvuI |
| BspCNI |
CTCAG (9/7) |
BspCNI |
R0624 |
CTCAG (9/7) |
BseMII^ |
| BspDI |
AT/CGAT |
BspDI |
R0557 |
AT/CGAT |
BanIII, Bsa29I, BseCI, Bsp106I, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI |
| |
|
ClaI |
R0197 |
AT/CGAT |
|
| BspEI |
T/CCGGA |
BspEI |
R0540 |
T/CCGGA |
AccIII, BseAI, Bsp13I, Kpn2I, MroI |
| BspGIx |
CTGGAC |
|
|
|
|
| BspHI |
T/CATGA |
BspHI |
R0517 |
T/CATGA |
PagI, RcaI |
| BspLI |
GGN/NCC |
NlaIV |
R0126 |
GGN/NCC |
NlaIV, PspN4I |
| BspLU11I |
A/CATGT |
PciI |
V0275 |
A/CATGT |
PciI |
| BspMI |
ACCTGC (4/8) |
BfuAI |
R0701 |
ACCTGC (4/8) |
Acc36I, BfuAI, BveI |
| |
|
BspMI |
R0502 |
ACCTGC (4/8) |
|
| BspMIIx |
T/CCGGA |
BspEI |
R0540 |
T/CCGGA |
AccIII, BseAI, Bsp13I, BspEI, Kpn2I, MroI |
| BspMAI |
CTGCA/G |
PstI |
R0140 |
CTGCA/G |
PstI |
| BspNCIx |
CCAGA |
|
|
|
|
| BspPI |
GGATC (4/5) |
AlwI |
R0513 |
GGATC (4/5) |
AclWI, AlwI |
| BspQI |
GCTCTTC (1/4) |
BspQI |
R0569 |
GCTCTTC (1/4) |
SapI |
| |
|
SapI |
R0569 |
GCTCTTC (1/4) |
|
| BspTI |
C/TTAAG |
AflII |
R0520 |
C/TTAAG |
AflII, BfrI, Bst98I, MspCI,Vha464I |
| BspT104I |
TT/CGAA |
BstBI |
R0519 |
TT/CGAA |
AsuII, Bpu14I, Bsp119I, BstBI, Csp45I, NspV,SfuI |
| BspT107I |
G/GYRCC |
BanI |
R0118 |
G/GYRCC |
AccB1I, BanI, BshNI |
| BspTNI |
GGTCTC (1/5) |
BsaI |
R0535 |
GGTCTC (1/5) |
BsaI, Bso31I, Eco31I |
| BspXI |
AT/CGAT |
BspDI |
R0557 |
AT/CGAT |
BanIII, Bsa29I, BseCI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, ZhoI |
| |
|
ClaI |
R0197 |
AT/CGAT |
|
| BsrI |
ACTGG (1/-1) |
BsrI |
R0527 |
ACTGG (1/-1) |
Bse1I, BseNI, BsrSI |
| BsrBI |
CCGCTC (-3/-3) |
BsrBI |
R0102 |
CCGCTC (-3/-3) |
AccBSI, MbiI |
| BsrDI |
GCAATG (2/0) |
BsrDI |
R0574 |
GCAATG (2/0) |
Bse3DI, BseMI |
| BsrFI |
R/CCGGY |
BsrFI |
R0562 |
R/CCGGY |
Bse118I, BssAI, Cfr10I |
| BsrGI |
T/GTACA |
BsrGI |
R0575 |
T/GTACA |
Bsp1407I, BstAUI, SspBI |
| BsrSI |
ACTGG (1/-1) |
BsrI |
R0527 |
ACTGG (1/-1) |
Bse1I, BseNI, BsrI |
| BssAI |
R/CCGGY |
BsrFI |
R0562 |
R/CCGGY |
Bse118I, BsrFI, Cfr10I |
| BssECI |
C/CNNGG |
BsaJI |
R0536 |
C/CNNGG |
BsaJI, BseDI |
| BssHI |
C/TCGAG |
PaeR7I |
R0177 |
C/TCGAG |
PaeR7I, Sfr274I, SlaI, TliI, XhoI |
| |
|
TliI |
R0612 |
C/TCGAG |
|
| |
|
XhoI |
R0146 |
C/TCGAG |
|
| BssHII |
G/CGCGC |
BssHII |
R0199 |
G/CGCGC |
BsePI, PauI |
| BssKI |
/CCNGG |
BssKI |
R0592 |
/CCNGG |
Bme1390I^, BstSCI, MspR9I^, ScrFI^,
StyD4I |
| |
|
ScrFI^ |
R0110 |
CC/NGG |
|
| |
|
StyD4I |
R0638 |
/CCNGG |
|
| BssNAI |
GTA/TAC |
BstZ17I |
R0594 |
GTA/TAC |
Bst1107I, BstZ17I |
| BssSI |
CACGAG (-5/-1) |
BssSI |
R0587 |
CACGAG (-5/-1) |
Bst2BI |
| BssT1I |
C/CWWGG |
StyI |
R0500 |
C/CWWGG |
Eco130I, EcoT14I, ErhI, StyI |
| Bst6I |
CTCTTC (1/4) |
EarI |
R0528 |
CTCTTC (1/4) |
Eam1104I, EarI, Ksp632I |
| Bst98I |
C/TTAAG |
AflII |
R0520 |
C/TTAAG |
AflII, BfrI, BspTI, MspCI,Vha464I |
| Bst1107I |
GTA/TAC |
BstZ17I |
R0594 |
GTA/TAC |
BssNAI, BstZ17I |
| BstACI |
GR/CGYC |
BsaHI |
R0556 |
GR/CGYC |
AcyI, BsaHI, Hin1I, Hsp92I |
| BstAPI |
GCANNNN/NTGC |
BstAPI |
V0259 |
GCANNNN/NTGC |
|
| BstBI |
TT/CGAA |
BstBI |
R0519 |
TT/CGAA |
AsuII, Bpu14I, Bsp119I, BspT104I, Csp45I, NspV,SfuI |
| Bst2BI |
CACGAG (-5/-1) |
BssSI |
R0587 |
CACGAG (-5/-1) |
BssSI |
| BstBAI |
YAC/GTR |
BsaAI |
R0531 |
YAC/GTR |
BsaAI |
| Bst4CI |
ACN/GT |
HpyCH4III |
R0618 |
ACN/GT |
HpyCH4III, TaaI |
| BstC8I |
GCN/NGC |
Cac8I |
R0579 |
GCN/NGC |
Cac8I |
| BstDEI |
C/TNAG |
DdeI |
R0175 |
C/TNAG |
DdeI |
| BstDSI |
C/CRYGG |
BtgI |
R0608 |
C/CRYGG |
BtgI |
| BstEII |
G/GTNACC |
BstEII |
R0162 |
G/GTNACC |
BstPI, | |
