Frequencies of Restriction Sites, NEB

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Home > Technical Reference > Restriction Endonucleases > Frequencies of Restriction Sites



Frequencies of Restriction Sites

The table below summarizes the frequencies with which restriction endonuclease sites occur in commonly used DNA molecules. Detailed restriction maps can be found on DNA sequences and maps. The sites listed in these tables were identified by computer analysis of published sequences. Although we have tried to ensure their accuracy, the sites have not necessarily been confirmed by experimentation. When the same specificity is displayed by several enzymes, the site is listed by the name of the enzyme that is available from New England Biolabs. Other enzymes with the same specificity are listed in the table of isoschizomers. Recognition sequences are written 5´ to 3´.

A | B | C | D | E | F | H | K | L | M | N | P | R | S | T | X | Z |

Enzyme	Sequence	Adeno-2		LITMUS 28i		pBR 322		pGLuc- Basic		pMAL- p5X		pTXB1		pUC19
Enzyme	Sequence	Lambda		M13 mp18		pGLuc- Basic2		pKLAC2		pSNAP_f		pTYB 21		T7
AatII	GACGTC	3	10	1	0	1	1	1	0	0	5	1	0	1	1
Acc65I	GGTACC	8	2	1	1	0	1	1	0	0	2	0	1	1	5
AccI	GTMKAC	17	9	0	1	2	3	3	5	1	2	5	3	1	33
AciI	CCGC	582	516	27	42	67	66	64	81	80	75	102	92	34	199
AclI	AACGTT	3	7	3	2	4	2	2	2	5	3	12	9	2	19
AcuI	CTGAAG	23	40	2	0	2	9	4	8	4	5	2	2	2	1
AfeI	AGCGCT	13	2	0	1	4	0	0	0	2	0	1	2	0	0
AflII	CTTAAG	4	3	0	0	0	0	0	0	0	0	0	0	0	19
AflIII	ACRYGT	25	20	1	3	1	5	1	4	2	5	3	4	1	23
AgeI	ACCGGT	5	13	1	0	0	0	0	1	0	1	1	0	0	2
AgeI-HF™	ACCGGT	5	13	1	0	0	-	0	1	0	-	1	0	0	2
AhdI	GACNNNNNGTC	9	9	2	0	1	1	2	1	2	1	2	2	1	14
AleI	CACNNNNGTG	10	20	0	1	0	0	0	1	0	1	0	2	0	8
AluI	AGCT	158	143	15	27	17	31	25	38	28	27	30	34	16	140
AlwI	GGATC	35	58	10	3	12	21	20	18	12	20	15	16	10	1
AlwNI	CAGNNNCTG	25	41	1	1	1	2	2	5	2	4	1	2	1	15
ApaI	GGGCCC	12	1	0	0	0	0	0	0	1	1	1	1	0	0
ApaLI	GTGCAC	7	4	2	0	3	2	3	3	6	4	4	4	3	1
ApeKI	GCWGC	179	199	9	10	21	22	23	27	25	20	27	29	12	116
ApoI	RAATTY	29	58	3	11	0	3	3	19	5	3	5	7	1	13
AscI	GGCGCGCC	2	2	0	0	0	0	0	0	0	1	0	0	0	0
AseI	ATTAAT	3	17	1	7	1	4	4	5	4	7	10	4	3	12
AsiSI	GCGATCGC	1	0	0	0	0	0	0	0	0	0	0	0	0	0
AvaI	CYCGRG	40	8	2	2	1	2	3	2	1	2	3	1	1	4
AvaII	GGWCC	73	35	2	1	8	4	3	7	9	6	6	8	2	54
AvrII	CCTAGG	2	2	1	0	0	1	1	0	0	1	0	0	0	3
BaeGI <top>	GKGCMC	45	10	2	1	3	4	5	8	8	8	6	5	3	16
BaeI	ACNNNNGTAYC	5	10	1	3	0	1	0	1	0	0	0	1	0	3
BamHI	GGATCC	3	5	1	1	1	1	1	1	1	1	1	1	1	0
BamHI-HF^™	GGATCC	3	5	1	1	1	-	1	1	1	-	1	1	1	0
BanI	GGYRCC	57	25	4	7	9	7	6	7	4	8	8	12	4	33
BanII	GRGCYC	57	7	2	2	2	5	6	5	2	5	3	6	1	1
BbsI	GAAGAC	27	24	0	0	3	2	1	3	3	2	4	4	0	38
BbvCI	CCTCAGC	9	7	0	2	0	0	0	0	0	0	0	0	0	10
BbvI	GCAGC	179	199	9	10	21	22	23	27	25	20	27	29	12	116
BccI	CCATC	62	145	5	14	9	25	11	22	16	8	14	25	3	121
BceAI	ACGGC	80	115	3	7	3	7	5	13	11	8	13	10	2	47
BcgI	CGANNNNNNTGC	10	28	1	0	3	1	1	6	4	1	4	6	1	19
BciVI	GTATCC	9	26	2	0	2	4	4	3	4	3	4	4	2	23
BclI	TGATCA	5	8	0	0	0	2	1	2	2	1	1	2	0	1
BcoDI	GTCTC	60	37	3	5	3	9	3	11	8	4	8	9	4	95
BfaI	CTAG	54	13	7	5	5	12	10	19	3	14	8	8	4	60
BfuAI	ACCTGC	39	41	0	3	1	5	4	5	4	4	2	3	1	18
BfuCI	GATC	87	116	16	6	22	38	33	35	23	31	24	29	15	6
BglI	GCCNNNNNGGC	20	29	2	1	3	1	1	3	1	7	2	3	2	2
BglII	AGATCT	11	6	1	1	0	3	1	1	1	2	0	2	0	1
BlpI	GCTNAGC	8	6	0	0	0	0	0	0	1	0	1	1	0	20
BmgBI	CACGTC	15	17	0	0	0	1	0	1	1	2	0	0	0	8
BmrI	ACTGGG	22	4	2	1	5	-	3	2	5	-	11	8	2	6
BmtI	GCTAGC	4	1	0	0	1	0	0	4	0	1	1	1	0	1
BpmI	CTGGAG	32	25	1	2	4	6	4	3	4	1	5	6	1	23
Bpu10I	CCTNAGC	23	19	0	4	1	0	0	0	1	2	0	2	0	39
BpuEI	CTTGAG	19	13	4	4	6	6	6	7	5	9	7	9	4	56
BsaAI	YACGTR	22	14	2	5	1	4	2	4	0	3	2	4	0	35
BsaBI	GATNNNNATC	2	21	0	2	1	4	2	2	2	1	1	3	0	7
BsaHI	GRCGYC	44	40	2	1	6	5	5	6	5	8	12	7	3	8
BsaI	GGTCTC	18	2	1	0	1	1	1	3	2	2	2	2	1	29
BsaI-HF™	GGTCTC	18	2	1	0	1	-	1	3	2	-	2	2	1	29
BsaJI	CCNNGG	234	105	6	9	8	16	21	18	10	17	15	15	5	85
BsaWI	WCCGGW	28	81	4	6	5	4	4	8	7	5	8	6	3	32
BsaXI	ACNNNNNCTCC	29	19	1	4	0	4	3	3	1	1	2	3	1	12
BseRI	GAGGAG	63	19	0	1	0	2	1	2	0	3	0	0	0	13
BseYI	CCCAGC	31	32	1	3	2	5	5	3	4	5	4	4	1	29
BsgI	GTGCAG	34	41	0	0	1	1	3	4	6	3	5	4	0	21
BsiEI	CGRYCG	50	22	4	3	7	6	7	11	8	6	9	8	5	17
BsiHKAI	GWGCWC	38	28	4	3	8	6	8	8	9	9	7	7	5	24
BsiWI	CGTACG	4	1	1	0	0	0	0	0	1	0	1	0	0	0
BslI	CCNNNNNNNGG	216	176	8	17	20	21	15	18	16	26	31	25	6	90
BsmAI	GTCTC	60	37	3	5	3	-	3	11	8	-	8	10	4	95
BsmBI	CGTCTC	21	14	1	1	1	2	0	2	2	0	2	2	2	16
BsmFI	GGGAC	59	38	0	2	4	9	7	4	1	5	4	6	0	46
BsmI	GAATGC	10	46	1	1	1	4	1	3	1	5	1	0	0	15
BsoBI	CYCGRG	40	8	2	2	1	2	3	2	1	2	3	1	1	4
Bsp1286I	GDGCHC	105	38	5	5	10	12	15	16	11	15	11	12	5	40
BspCNI	CTCAG	75	80	4	24	7	10	8	10	10	9	20	16	5	142
BspDI	ATCGAT	2	15	0	2	1	0	0	2	0	0	0	0	0	3
BspEI	TCCGGA	8	24	0	0	1	0	0	1	2	0	1	1	0	0
BspHI	TCATGA	3	8	2	1	4	2	2	2	1	2	2	3	3	13
BspMI	ACCTGC	39	41	0	3	1	5	4	5	4	4	2	3	1	18
BspQI	GCTCTTC	7	10	0	0	1	3	3	2	1	3	1	1	1	4
BsrBI	CCGCTC	28	17	3	4	2	6	7	6	4	6	9	5	3	17
BsrDI	GCAATG	14	44	2	3	2	4	3	7	4	4	4	4	2	18
BsrFI	RCCGGY	40	61	2	1	7	6	6	9	2	6	11	9	1	3
BsrGI	TGTACA	5	5	0	1	0	0	0	1	0	1	1	2	0	13
BsrI	ACTGG	86	110	11	19	18	17	14	23	26	19	32	28	11	118
BssHII	GCGCGC	52	6	1	0	0	1	1	1	2	2	1	1	0	1
BssKI	CCNGG	233	185	9	11	16	28	24	25	27	28	46	29	12	11
BssSI	CACGAG	11	8	2	0	3	5	4	5	3	4	2	4	3	31
BstAPI	GCANNNNNTGC	20	34	0	0	2	2	2	3	2	0	3	2	1	12
BstBI	TTCGAA	1	7	1	0	0	1	1	2	0	0	0	1	0	7
BstEII	GGTNACC	10	13	0	0	0	1	0	1	1	0	1	1	0	1
BstNI	CCWGG	136	71	5	7	6	19	14	15	14	19	19	14	5	2
BstUI	CGCG	303	157	11	17	23	15	17	26	31	19	41	35	10	65
BstXI	CCANNNNNNTGG	10	13	0	0	0	2	0	2	4	1	4	3	0	11
BstYI	RGATCY	22	21	8	2	8	16	14	12	9	11	10	12	7	2
BstZ17I	GTATAC	3	3	0	0	1	1	1	3	0	1	1	1	0	8
Bsu36I	CCTNAGG	7	2	0	1	0	1	0	1	1	1	0	0	0	30
BtgI	CCRYGG	82	46	1	2	2	2	7	4	3	6	1	4	0	26
BtgZI	GCGATG	23	45	2	4	3	7	6	4	6	6	4	3	0	24
BtsCI	GGATG	78	150	4	4	12	16	11	20	17	7	12	18	5	97
BtsI	GCAGTG	22	34	2	1	2	4	5	7	5	5	4	4	3	20
BtsIMutI	CAGTG	-	-	-	-	-	-	-	-	-	-	-	-	-	-
Cac8I <top>	GCNNGC	285	238	12	28	31	29	33	33	32	41	49	42	14	104
ClaI	ATCGAT	2	15	0	2	1	0	0	2	0	0	0	0	0	3
CspCI	CAANNNNNGTGG	6	7	0	1	0	0	0	1	0	1	0	0	0	9
CviAII	CATG	183	181	9	14	26	25	23	38	23	21	23	31	11	148
CviKI-1	RGCY	680	692	46	103	73	105	101	131	86	119	112	121	45	562
CviQI	GTAC	83	113	3	19	3	11	3	15	7	14	6	11	3	168
DdeI <top>	CTNAG	97	104	4	30	8	11	9	17	11	11	20	19	6	282
DpnI	GATC	87	116	16	6	22	38	33	35	23	31	24	29	15	6
DpnII	GATC	87	116	16	6	22	38	33	35	23	31	24	29	15	6
DraI	TTTAAA	12	13	2	5	3	3	3	5	1	6	3	2	3	9
DraIII	CACNNNGTG	10	10	1	1	0	1	1	2	0	3	1	1	0	16
DraIII-HF™	CACNNNGTG	10	10	1	1	0	-	1	2	0	-	1	1	0	16
DrdI	GACNNNNNNGTC	6	3	2	1	2	3	3	5	2	2	4	4	2	11
EaeI <top>	YGGCCR	70	39	2	3	6	8	10	10	5	15	4	8	3	2
EagI	CGGCCG	19	2	0	0	1	2	2	4	1	2	2	2	0	0
EagI-HF^™	CGGCCG	19	2	0	0	1	-	2	4	1	-	2	2	0	0
EarI	CTCTTC	29	34	2	2	2	5	4	11	6	4	3	5	3	46
EciI	GGCGGA	29	32	3	2	4	10	9	6	6	8	9	8	3	2
Eco53kI	GAGCTC	16	2	1	1	0	1	1	2	1	2	0	1	1	0
EcoNI	CCTNNNNNAGG	10	9	0	0	1	0	0	3	0	0	2	1	0	1
EcoO109I	RGGNCCY	44	3	0	0	4	1	0	1	2	5	1	3	1	22
EcoP15I	CAGCAG	50	72	3	4	7	4	4	7	10	6	6	5	3	36
EcoRI	GAATTC	5	5	1	1	1	1	1	1	1	1	1	1	1	0
EcoRI-HF^™	GAATTC	5	5	1	1	1	-	1	1	1	-	1	1	1	0
EcoRV	GATATC	9	21	1	0	1	1	1	1	1	1	1	3	0	0
EcoRV-HF^™	GATATC	9	21	1	0	1	-	1	1	1	-	1	3	0	0
FatI <top>	CATG	183	181	9	14	26	25	23	38	23	21	23	31	11	148
FauI	CCCGC	147	90	5	10	10	10	10	14	17	11	28	22	5	24
Fnu4HI	GCNGC	411	380	16	17	42	46	46	52	42	47	49	52	19	156
FokI	GGATG	78	150	4	4	12	16	11	20	17	7	12	18	5	97
FseI	GGCCGGCC	3	0	0	0	0	0	0	0	0	0	0	0	0	0
FspEI	CC	-	-	-	-	-	-	-	-	-	-	-	-	-	-
FspI	TGCGCA	17	15	2	1	4	2	2	3	2	2	1	2	2	7
HaeII <top>	RGCGCY	76	48	4	6	11	5	5	6	9	3	7	12	3	26
HaeIII	GGCC	216	149	12	15	22	21	24	31	23	36	34	36	11	68
HgaI	GACGC	87	102	3	7	11	5	6	10	12	7	20	15	4	70
HhaI	GCGC	375	215	19	26	31	-	28	36	39	-	41	46	17	103
HincII	GTYRAC	25	35	2	1	2	3	3	9	7	4	7	6	1	61
HindIII	AAGCTT	12	6	1	1	1	1	1	1	1	4	0	2	1	0
HindIII-HF™	AAGCTT	12	6	1	1	1	-	1	1	1	-	0	2	1	0
HinfI	GANTC	72	148	6	26	10	21	15	31	9	11	16	20	6	218
HinP1I	GCGC	375	215	19	26	31	31	28	36	39	27	41	46	17	103
HpaI	GTTAAC	6	14	1	0	0	0	0	3	1	1	1	2	0	18
HpaII	CCGG	171	328	12	18	26	24	25	32	25	24	50	35	13	58
HphI	GGTGA	99	168	9	18	12	11	8	15	19	14	21	20	7	102
Hpy166II	GTNNAC	116	125	10	10	8	14	13	29	20	13	27	24	5	199
Hpy188I	TCNGA	80	170	10	31	15	24	20	24	19	17	19	28	10	153
Hpy188III	TCNNGA	103	185	12	28	19	32	26	32	22	25	27	28	13	173
Hpy99I	CGWCG	61	102	5	8	9	12	12	14	9	13	18	16	5	29
HpyAV	CCTTC	84	106	7	14	10	16	12	24	14	11	16	20	6	110
HpyCH4III	ACNGT	122	187	7	31	14	22	11	25	20	15	18	22	8	174
HpyCH4IV	ACGT	83	143	13	22	10	15	11	21	10	19	23	22	5	170
HpyCH4V	TGCA	207	273	10	18	21	32	28	39	28	30	26	27	13	116
KasI <top>	GGCGCC	20	1	0	1	4	1	1	1	1	1	1	4	1	2
KpnI	GGTACC	8	2	1	1	0	1	1	0	0	2	0	1	1	5
KpnI-HF™	GGTACC	8	2	1	1	0	-	1	0	0	-	0	1	1	5
LpnPI <top>	CCDG	-	-	-	-	-	-	-	-	-	-	-	-	-	-
MboI <top>	GATC	87	116	16	6	22	38	33	35	23	31	24	29	15	6
MboII	GAAGA	113	130	10	10	11	18	13	38	15	14	14	18	8	140
MfeI	CAATTG	4	8	0	0	0	0	0	2	1	2	1	1	0	8
MfeI-HF^™	CAATTG	4	8	0	0	0	-	0	2	1	-	1	1	0	8
MluCI	AATT	87	189	8	62	8	18	15	43	22	19	31	44	7	79
MluI	ACGCGT	5	7	0	0	0	0	0	0	1	2	2	1	0	1
MlyI	GAGTC	40	61	6	8	4	8	8	17	5	6	11	10	4	115
MmeI	TCCRAC	25	18	3	3	4	5	3	8	3	5	4	4	2	33
MnlI	CCTC	397	262	11	62	26	35	30	56	24	41	35	39	13	342
MscI	TGGCCA	17	18	0	1	1	1	2	0	1	2	0	1	0	2
MseI	TTAA	115	195	15	63	15	18	16	41	24	23	32	39	13	207
MslI	CAYNNNNRTG	35	62	3	3	7	7	5	10	10	6	7	9	3	38
MspA1I	CMGCKG	95	75	4	4	6	9	8	14	11	8	10	12	6	35
MspI	CCGG	171	328	12	18	26	24	25	32	25	24	50	35	13	58
MspJI	CNNR	-	-	-	-	-	-	-	-	-	-	-	-	-	-
MwoI	GCNNNNNNNGC	391	347	13	19	34	33	38	33	30	42	41	43	13	170
NaeI <top>	GCCGGC	13	1	0	1	4	3	3	3	0	2	5	5	0	0
NarI	GGCGCC	20	1	0	1	4	1	1	1	1	1	1	4	1	2
Nb.BbvCI	CCTCAGC	9	7	0	2	0	-	0	0	0	-	0	0	0	10
Nb.BsmI	GAATGC	10	46	1	1	1	-	1	3	1	-	1	0	0	15
Nb.BsrDI	GCAATG	14	44	2	3	2	-	3	7	4	-	4	4	2	18
Nb.BtsI	GCAGTG	22	34	2	1	2	-	5	7	5	-	4	4	3	20
NciI	CCSGG	97	114	4	4	10	9	10	10	13	9	27	15	7	9
NcoI	CCATGG	20	4	1	0	0	2	4	1	1	3	0	1	0	1
NcoI-HF^™	CCATGG	20	4	1	0	0	-	4	1	1	-	0	1	0	1
NdeI	CATATG	2	7	0	3	1	0	0	1	1	1	1	1	1	7
NgoMIV	GCCGGC	13	1	0	1	4	3	3	3	0	2	5	5	0	0
NheI	GCTAGC	4	1	0	0	1	-	0	4	0	-	1	1	0	1
NheI-HF^™	GCTAGC	4	1	0	0	1	-	0	4	0	-	1	1	0	1
NlaIII	CATG	183	181	9	14	26	25	23	38	23	21	23	31	11	148
NlaIV	GGNNCC	178	82	13	18	24	25	18	22	14	20	22	30	11	99
NmeAIII	GCCGAG	17	8	1	0	3	3	4	2	3	2	2	4	1	14
NotI	GCGGCCGC	7	0	0	0	0	1	1	1	1	1	1	1	0	0
NotI-HF^™	GCGGCCGC	7	0	0	0	0	-	1	1	1	-	1	1	0	0
NruI	TCGCGA	5	5	0	0	1	0	1	1	0	1	1	0	0	3
NsiI	ATGCAT	9	14	1	0	0	3	3	6	0	1	0	0	0	8
NspI	RCATGY	41	32	1	6	4	7	6	9	3	5	5	5	3	24
Nt.AlwI	GGATC	35	58	10	3	12	-	20	18	12	-	15	16	10	1
Nt.BbvCI	CCTCAGC	9	7	0	2	0	-	0	0	0	-	0	0	0	10
Nt.BsmAI	GTCTC	60	37	3	5	3	-	3	11	8	-	8	10	4	95
Nt.BspQI	GCTCTTC	7	10	0	0	1	-	3	2	1	-	1	1	1	4
Nt.BstNBI	GAGTC	40	61	6	8	4	8	8	17	5	6	11	10	4	115
Nt.CviPII	CCD	4148	4641	261	570	457	595	511	806	570	609	716	729	251	3575
PacI <top>	TTAATTAA	1	0	0	1	0	0	0	0	0	1	0	0	0	1
PaeR7I	CTCGAG	6	1	1	0	0	1	2	1	0	1	1	0	0	0
PciI	ACATGT	9	2	1	3	1	2	1	3	1	2	1	1	1	6
PflFI	GACNNNGTC	12	2	0	0	1	2	1	1	1	1	1	2	0	1
PflMI	CCANNNNNTGG	18	14	0	0	2	3	0	3	1	5	2	2	0	8
PhoI	GGCC	216	149	12	15	22	21	24	31	23	36	34	36	11	68
PleI	GAGTC	40	61	6	8	4	8	8	17	5	6	11	10	4	115
PmeI	GTTTAAAC	1	2	0	0	0	0	0	0	0	1	1	0	0	2
PmlI	CACGTG	10	3	0	0	0	1	0	0	0	1	0	1	0	1
PpuMI	RGGWCCY	23	3	0	0	2	1	0	1	2	1	0	0	0	12
PshAI	GACNNNNGTC	2	7	0	0	1	1	0	1	0	0	1	2	0	6
PsiI	TTATAA	4	12	1	2	0	2	2	2	1	1	1	1	0	5
PspGI	CCWGG	136	71	5	7	6	19	14	15	14	19	19	14	5	2
PspOMI	GGGCCC	12	1	0	0	0	0	0	0	1	1	1	1	0	0
PspXI	VCTCGAGB	3	1	0	0	0	1	2	0	0	1	1	0	0	0
PstI	CTGCAG	30	28	1	1	1	2	2	3	1	4	1	1	1	0
PstI-HF™	CTGCAG	30	28	1	1	1	-	2	3	1	-	1	1	1	0
PvuI	CGATCG	7	3	2	1	1	1	2	3	2	1	1	2	2	0
PvuI-HF™	CGATCG	7	3	2	1	1	-	2	3	2	-	1	2	2	0
PvuII	CAGCTG	24	15	1	3	1	5	3	3	3	3	3	4	2	3
PvuII-HF^™	CAGCTG	24	15	1	3	1	-	3	3	3	-	3	4	2	3
RsaI <top>	GTAC	83	113	3	19	3	11	3	15	7	14	6	11	3	168
RsrII	CGGWCCG	2	5	0	0	0	1	1	0	1	1	0	0	0	1
SacI <top>	GAGCTC	16	2	1	1	0	12	1	2	1	2	0	1	1	0
SacI-HF^™	GAGCTC	16	2	1	1	0	-	1	2	1	-	0	1	1	0
SacII	CCGCGG	33	4	0	0	0	0	1	2	0	1	1	1	0	0
SalI	GTCGAC	3	2	0	1	1	1	3	1	1	1	4	1	1	0
SalI-HF^™	GTCGAC	3	2	0	1	1	-	3	1	1	-	4	1	1	0
SapI	GCTCTTC	7	10	0	0	1	3	3	2	1	3	1	1	1	4
Sau3AI	GATC	87	116	16	6	22	38	33	35	23	31	24	29	15	6
Sau96I	GGNCC	164	74	6	4	15	7	8	14	20	21	26	24	6	79
SbfI	CCTGCAGG	3	5	1	1	0	0	0	1	1	1	0	1	1	0
SbfI-HF^™	CCTGCAGG	3	5	1	1	0	-	0	1	1	-	0	1	1	0
ScaI	AGTACT	5	5	1	0	1	1	1	2	1	2	1	2	1	4
ScaI-HF^™	AGTACT	5	5	1	0	1	-	1	2	1	-	1	2	1	4
ScrFI	CCNGG	233	185	9	11	16	28	24	25	27	28	46	29	12	11
SexAI	ACCWGGT	9	5	0	0	0	2	1	3	0	0	0	0	0	0
SfaNI	GCATC	85	169	5	7	22	20	19	18	20	17	23	19	8	96
SfcI	CTRYAG	47	38	7	7	4	9	6	9	4	10	6	8	4	48
SfiI	GGCCNNNNNGGCC	3	0	0	0	0	0	0	0	0	1	0	0	0	1
SfoI	GGCGCC	20	1	0	1	4	1	1	1	1	1	1	4	1	2
SgrAI	CRCCGGYG	6	6	0	0	1	0	0	0	0	0	1	1	0	0
SmaI	CCCGGG	12	3	0	1	0	1	1	1	0	1	0	0	1	0
SmlI	CTYRAG	29	17	5	4	6	7	8	8	5	10	8	9	4	75
SnaBI	TACGTA	0	1	1	1	0	0	0	1	0	1	0	0	0	13
SpeI	ACTAGT	3	0	0	0	0	0	0	0	0	1	1	1	0	2
SphI	GCATGC	8	6	0	1	1	4	5	2	0	2	2	1	1	0
SphI-HF^™	GCATGC	8	6	0	1	1	-	5	2	0	-	2	1	1	0
SspI	AATATT	5	20	3	6	1	1	1	6	2	1	3	5	1	6
SspI-HF^™	AATATT	5	20	3	6	1	-	1	6	2	-	3	5	1	6
StuI	AGGCCT	11	6	1	0	0	1	1	1	0	0	1	1	0	1
StyD4I	CCNGG	233	185	9	11	16	28	24	25	27	28	46	29	12	11
StyI	CCWWGG	44	10	2	0	1	5	5	4	1	4	2	4	0	36
StyI-HF™	CCWWGG	44	10	2	0	1	-	5	4	1	-	2	4	0	36
SwaI	ATTTAAAT	1	0	1	1	0	0	0	0	0	1	1	1	0	1
Taq^αI <top>	TCGA	50	121	5	12	7	17	17	32	16	15	22	17	4	111
TfiI	GAWTC	32	87	0	18	6	13	7	14	4	5	5	10	2	103
TliI	CTCGAG	6	1	1	0	0	1	2	1	0	1	1	0	0	0
TseI	GCWGC	179	199	9	10	21	22	23	27	25	20	27	29	12	116
Tsp45I	GTSAC	73	81	6	9	9	8	6	9	7	5	12	13	4	108
Tsp509I	AATT	87	189	8	62	8	-	15	43	22	-	31	33	7	79
TspMI	CCCGGG	12	3	0	1	0	1	1	1	0	1	0	0	1	0
TspRI	CASTG	83	119	7	9	11	15	12	22	14	16	16	15	10	94
Tth111I	GACNNNGTC	12	2	0	0	1	2	1	1	1	1	1	2	0	1
XbaI <top>	TCTAGA	5	1	1	1	0	1	1	1	0	1	1	1	1	3
XcmI	CCANNNNNNNNNTGG	14	12	0	0	0	1	0	1	3	0	3	4	0	8
XhoI	CTCGAG	6	1	1	0	0	1	2	1	0	1	1	0	0	0
XmaI	CCCGGG	12	3	0	1	0	1	1	1	0	1	0	0	1	0
XmnI	GAANNNNTTC	5	24	0	2	2	3	4	3	1	3	7	2	1	12
ZraI <top>	GACGTC	3	10	1	0	1	1	1	0	0	5	1	0	1	1