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Home >  Technical Reference > Restriction Endonucleases Average Fragment Sizes Generated by Endonuclease Cleavage Average fragment sizes were generated from long contiguous stretches of DNA sequence available from public databases. For each organism, the length of DNA sequence evaluated was: E.coli, 4.6 Mb; M.tuberculosis, 4.4 Mb; P.abyssi, 1.8 Mb; S.cerevisiae, 12.1 Mb; A.thaliana, 1.8 Mb; C.elegans, 100 Mb; D.melanogaster, 3.8 Mb; M.musculus, 2.6 Mb and H.sapiens, 22 Mb.   Enzyme Sequence Escherichia coli Mycobacterium tuberculosis Pyrococcus abyssi Saccharomyces cerevisiae Arabidopsis thaliana Caenorhabditis elegans Drosophilia melanogaster Mus musculus Homo sapiens ApaI GGGCCC 68,000 3,000 5,000 15,000 42,000 38,000 13,000 5,000 5,000 AscI GGCGCGCC 28,000 9,000 350,000 450,000 920,000 510,000 200,000 280,000 670,000 AvrII CCTAGG 290,000 19,000 5,000 18,000 20,000 17,000 25,000 4,000 5,000 BamHI GGATCC 9,000 3,000 4,000 7,000 8,000 9,000 7,000 4,000 7,000 BbvCI CCTCAGC 34,000 13,000 11,000 29,000 29,000 56,000 25,000 7,000 4,000 BglI GCCNNNNNGGC 2,000 600 6,000 12,000 26,000 16,000 5,000 5,000 5,000 BssHII GCGCGC 2,000 900 100,000 27,000 120,000 14,000 12,000 19,000 33,000 DraI TTTAAA 3,000 120,000 2,000 1,000 900 500 800 1,000 1,000 EagI CGGCCG 16,000 600 11,000 27,000 37,000 17,000 11,000 30,000 32,000 EcoRI GAATTC 7,000 4,000 2,000 3,000 3,000 2,000 4,000 4,000 4,000 FseI GGCCGGCC 930,000 7,000 140,000 520,000 610,000 450,000 150,000 160,000 170,000 HindIII AAGCTT 8,000 18,000 1,000 3,000 2,000 3,000 3,000 4,000 4,000 NaeI GCCGGC 16,000 400 12,000 21,000 25,000 17,000 5,000 22,000 21,000 NarI GGCGCC 49,000 700 26000 15,000 47,000 14,000 7,000 17,000 11,000 NheI GCTAGC 30,000 6,000 6,000 10,000 12,000 19,000 10,000 7,000 11,000 NotI GCGGCCGC 200,000 4,000 180,000 290,000 610,000 260,000 83,000 120,000 130,000 PacI TTAATTAA 32,000 No Sites 42,000 28,000 8,000 14,000 7,000 34,000 21,000 PmeI GTTTAAAC 53,000 2,200,000 88,000 57,000 37,000 52,000 46,000 100,000 70,000 RsrII CGGWCCG 12,000 3,000 140,000 82,000 77,000 62,000 28,000 120,000 260,000 SacI GAGCTC 31,000 4,000 2,000 8,000 5,000 5,000 6,000 3,000 5,000 SacII CCGCGG 7,000 700 17,000 24,000 24,000 27,000 15,000 23,000 35,000 SalI GTCGAC 9,000 1,000 14,000 11,000 12,000 10,000 8,000 48,000 83,000 SapI GCTCTTC 13,000 22,000 5,000 12,000 12,000 14,000 17,000 9,000 14,000 SbfI CCTGCAGG 68,000 27,000 290,000 120,000 120,000 280,000 64,000 23,000 31,000 SfiI GGCCN5GGCC 150,000 4,000 57,000 170,000 460,000 280,000 81,000 23,000 62,000 SgrAI CRCCGGYG 8,000 900 55,000 46,000 34,000 59,000 27,000 98,000 170,000 SmaI CCCGGG 11,000 2,000 12,000 42,000 40,000 31,000 15,000 7,000 7,000 SpeI ACTAGT 59,000 45,000 8,000 5,000 5,000 8,000 10,000 9,000 8,000 SphI GCATGC 8,000 3,000 9,000 8,000 9,000 10,000 5,000 4,000 5,000 SrfI GCCCGGGC 120,000 10,000 220,000 570,000 No Sites 1,100,000 170,000 120,000 120,000 SspI AATATT 2,000 32,000 2,000 1,000 1,000 800 900 3,000 1,000 SwaI ATTTAAAT 40,000 4,411,523 29,000 18,000 12,000 8,000 6,000 31,000 15,000 XbaI TCTAGA 120,000 40,000 5,000 4,000 4,000 4,000 8,000 3,000 4,000 XhoI CTCGAG 40,000 3,000 2,000 9,000 6,000 9,000 7,000 15,000 22,000