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RELATED INFORMATION Technical Support New Products NEB Price List FAVORITE TOOLS Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home >  Technical Reference > Restriction Endonucleases Restriction Endonucleases Overview Quality, Performance and Convenience Restriction Endonucleases Overview Quality Control Overview Homing Endonucleases Nicking Endonucleases Time Saver Qualified Enzymes High Fidelity (HF) Restriction Enzymes NEB Enzyme List Why Choose Recombinant Enzymes? Optimizing Reactions Cloning Guide Troubleshooting Guide Avoiding Ribonuclease Contamination General Molecular Biology Data Publications General FAQs for Restriction Enzymes Product Specific MSDS, Manuals, Application Notes, FAQs and Protocols for Restriction Enzymes Product Specific MSDS, Manuals, Application Notes, FAQs and Protocols for Buffers Favorite Tools NEBcutter Enzyme Finder Double Digest Finder NEBuffer System NEBuffer Chart Double Digests Dilutent Buffer Compatibility The Switch to NEBuffer 4 and a Simplified Buffer System DNA Sequences and Maps DNA Sequences and Maps Frequencies of Restriction Sites Properties of Restriction Enzymes Activity in a PCR Mix Activity in a Vent PCR Mix Activity at 37°C for restriction enzymes with alternate incubation temperatures Cleavage Close to the End of DNA fragments (linearized vector) Cleavage Close to the End of DNA Fragments (oligonucleotides) Cleavage of Supercoiled DNA Heat Inactivation Site Preferences Star Activity Reduced Star Activities of HF Enzymes Extended Digests with Restriction Endonucleases Isoschizomers/Recognition Sequences Alphabetical List of Sequences Isoschizomers Cross Index of Recognition Sequences Interrupted Palindromes Nonpalindromic Sequences Multiple Recognition Sequences Sequences greater than 6 bases Generating New Cleavage Sites Recleavable Filled-in 5' Overhangs Recleavable Blunt Overhangs Compatible Cohesive Overhangs Alteration of Apparent Recognition Specificities using Methylases Methylases Dam, Dcm and CpG Methylases Procedure for Altering Cleavage Specificities using Methylases Restriction of Foreign DNA by E. coli K-12 Dam and Dcm Methylases of E. coli Genomic Analysis of DNA Megabase Mapping Digestion of Agarose-Embedded DNA Digestion of Agarose-Embedded DNA: Info for Specific Enzymes Average Fragment Size Generated by Endonuclease Cleavage Feature Articles Nicking Endonucleases: The Discovery and Engineering of Restriction Enzyme Variants Restriction Enzymes at NEB: Over 30 Years of Innovation