|
Sequences at the top of each column are written 5´ to 3´ according to convention. Open squares at the
left of each row are place holders for nucleotides within a restriction endonuclease recognition sequence; arrowheads
indicate the point of cleavage. Sequences of complementary strands and their cleavage sites are implied. Enzymes
written in blue type recognize only one sequence while enzymes written in black type
recognize multiple sequences.
Tetra- and Hexa-Nucleotide Recognition Sequences | Penta-Nucleotide
Recognition Sequences
Palindromic Tetra- and Hexa-Nucleotide Recognition Sequences PDF
of this table
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
 |
Tsp509I |
|
|
|
FatI |
|
|
|
BfuCI
DpnII
MboI
Sau3AI
|
|
|
|
|
|
|
|
 |
|
HpyCH4IV |
|
|
CviAII |
MspI
HpaII |
|
BfaI |
|
HinP1I |
|
Csp6I
CviQI |
|
TaqI |
|
MseI |
 |
|
|
AluI
CviKI-1 |
|
|
|
BstUI |
|
DpnI |
|
HaeIII
PhoI
CviKI-1 |
RsaI |
|
|
HpyCH4V |
|
 |
|
|
|
|
|
|
|
|
BstKTI |
HhaI |
|
|
|
|
|
|
 |
|
TaiI |
|
|
NlaIII |
|
|
|
|
|
|
|
|
|
|
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| A T |
ApoI |
|
HindIII |
|
PciI
AflIII |
AgeI
BsrFI
BsaWI |
MluI
AflIII |
SpeI |
BglII
BstYII |
|
|
TatI |
|
|
|
|
| A T |
|
AclI |
|
|
|
|
|
|
|
|
|
|
|
ClaI
BspDI |
|
AseI |
| A T |
|
|
|
SspI |
|
|
|
|
|
AfeI |
StuI |
ScaI |
|
|
|
|
| A T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| AT |
|
|
|
|
NspI |
|
|
|
|
HaeII |
|
|
|
|
NsiI
|
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| C G |
MfeI |
|
|
|
NcoI
StyI
BtgI
BsaJI |
TspMI
XmaI
AcoI
AvaI
BsaJI
BsoBI |
BsaJI
BtgI |
AvrII
BsaJI
StyI |
|
|
EagI
EaeI |
BsiWI |
SfcI |
PaeR7I
TliI
XhoI
AvaI
BsoBI
SmlI |
SfcI |
AflII
SmlI |
| C G |
|
|
|
NdeI |
|
BmeT110I |
|
|
|
|
|
|
|
BmeT110I |
|
|
| C G |
|
PmlI
BsaAI |
PvuII
MspA1I |
|
|
SmaI |
MspA1I |
|
|
|
|
|
|
|
|
|
| C G |
|
|
|
|
|
|
SacII |
|
PvuI
BsiEI |
|
BsiEI |
|
|
|
|
|
| C G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PstI |
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| G C |
EcoRI
ApoI |
|
|
|
|
NgoMIV
BsrFI |
BssHII |
NheI |
BamHI
BstYI |
KasI
BanI |
PspOMI |
Acc65I
BanI |
|
SalI |
ApaLI |
|
| G C |
|
BsaHI |
|
|
|
|
|
|
|
NarI
BsaHI |
|
|
AccI |
AccI |
|
|
| G C |
|
ZraI |
Ecl136II
Eco53KI
|
EcoRV |
Cac8I |
NaeI
Cac8I |
Cac8I |
Cac8I |
NlaIV |
SfoI
NlaIV |
NlaIV |
NlaIV |
BstZ17I
Hpy8I
Hpy166II
|
HincII
Hpy8I
Hpy166II |
Hpy8I
Hpy166II |
HpaI
HincII
Hpy8I
Hpy166II |
| G C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| G C |
|
AatII |
SacI
BanII
BsiHKAI
Bsp1286I |
|
SphI
NspI |
|
|
BmtI |
|
BbeI
HaeII |
ApaI
BanII
BaeGI
Bsp1286I |
KpnI |
|
|
Bme1580I
Bsp1286I
BsiHKAI |
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| T A |
|
|
|
|
BspHI |
BspEI
BsaWI
AcoI |
|
XbaI |
BclI |
|
EaeI |
BsrGI
TatI |
|
|
|
|
| T A |
|
|
|
|
Hpy188III |
Hpy188III |
Hpy188III |
Hpy188III |
|
|
|
|
|
BstBI |
|
|
| T A |
|
SnaBI
BsaAI |
|
|
|
|
NruI |
|
|
FspI |
MscI |
|
PsiI |
|
|
DraI |
| T A |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| TA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Palindromic Penta-Nucleotide Recognition Sequences PDF
of this table top
| |
AA TT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
 |
N |
 |
|
|
|
|
|
|
BssKI
StyD4I |
|
|
|
|
|
MaeIII |
|
|
|
|
 |
N |
|
|
|
|
|
|
|
|
|
DdeI |
HinfI |
|
Sau96I |
|
|
|
|
|
 |
N |
|
|
|
|
|
|
|
ScrFI |
|
|
|
Fnu4HI |
BmgT120I |
|
|
|
|
|
|
N |
|
|
|
HpyCH4II |
|
|
|
|
|
|
|
|
|
|
|
Hpy188I |
|
|
|
N |
|
|
|
|
|
|
|
|
|
|
|
|
FmuI |
|
|
|
|
|
|
N |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| |
A |
|
| T |
|
|
|
|
|
|
PspGI |
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
|
|
TfiI |
ApeKI
TseI |
AvaII |
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
BstNI |
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
Hpy99I |
|
|
|
|
|
|
|
|
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
Tsp45I |
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
NciI |
|
|
|
|
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
TauI
BspUI |
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
