|
Sequences at the top of each column are written 5´ to 3´ according to convention. Open squares at the
left of each row are place holders for nucleotides within a restriction endonuclease recognition sequence; arrowheads
indicate the point of cleavage. Sequences of complementary strands and their cleavage sites are implied. Enzymes
written in blue type recognize only one sequence while enzymes written in black type
recognize multiple sequences.
Tetra- and Hexa-Nucleotide Recognition Sequences | Penta-Nucleotide
Recognition Sequences
Palindromic Tetra- and Hexa-Nucleotide Recognition Sequences PDF
of this table
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
 |
Tsp509I |
|
|
|
FatI |
|
|
|
BfuCI
DpnII
MboI
Sau3AI
|
|
|
|
|
|
|
|
 |
|
HpyCH4IV |
|
|
CviAII |
MspI
HpaII |
|
BfaI |
|
HinP1I |
|
Csp6I |
|
TaqI |
|
MseI |
 |
|
|
AluI
CviJI |
|
|
|
BstUI |
|
DpnI |
|
HaeIII
PhoI
CviJI |
RsaI |
|
|
HpyCH4V |
|
 |
|
|
|
|
|
|
|
|
|
HhaI |
|
|
|
|
|
|
 |
|
TaiI |
|
|
NlaIII |
|
|
|
|
|
|
|
|
|
|
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| A T |
ApoI |
|
HindIII |
|
PciI
AflIII |
AgeI
BsrFI
BsaWI |
MluI
AflIII |
SpeI |
BglII
BstYI |
|
|
TatI |
|
|
|
|
| A T |
|
AclI |
|
|
|
|
|
|
|
|
|
|
|
ClaI
BspDI |
|
AseI |
| A T |
|
|
|
SspI |
|
|
|
|
|
AfeI |
StuI |
ScaI |
|
|
|
|
| A T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| AT |
|
|
|
|
NspI |
|
|
|
|
HaeII |
|
|
|
|
NsiI
BfrBI |
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| C G |
MfeI |
|
|
|
NcoI
StyI
BtgI |
XmaI
AvaI
BsoBI |
BtgI |
AvrII
StyI |
|
|
EagI
EaeI |
BsiWI |
SfcI |
PaeR7I
TliI
XhoI
AvaI
BsoBI
SmlI |
SfcI |
AflII
SmlI |
| C G |
|
|
|
NdeI |
|
|
|
|
|
|
|
|
|
|
|
|
| C G |
|
PmlI
BsaAI |
PvuII
MspA1I |
|
|
SmaI |
MspA1I |
|
|
|
|
|
|
|
|
|
| C G |
|
|
|
|
|
|
SacII |
|
PvuI
BsiEI |
|
BsiEI |
|
|
|
|
|
| C G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PstI |
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| G C |
EcoRI
ApoI |
|
|
|
|
NgoMIV
BsrFI |
BssHII |
NheI |
BamHI
BstYI |
KasI
BanI |
PspOMI |
Acc65I
BanI |
|
SalI |
ApaLI |
|
| G C |
|
BsaHI |
|
|
|
|
|
|
|
NarI
BsaHI |
|
|
AccI |
AccI |
|
|
| G C |
|
ZraI |
Ecl136II |
EcoRV |
|
NaeI |
|
|
|
SfoI |
|
|
BstZ17I |
HincII |
|
HpaI
HincII |
| G C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| G C |
|
AatII |
SacI
BanII
BsiHKAI
Bsp1286I |
|
SphI
NspI |
|
|
BmtI |
|
BbeI
HaeII |
ApaI
BanII
Bme1580I
Bsp1286I |
KpnI |
|
|
Bme1580I
Bsp1286I
BsiHKAI |
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| T A |
|
|
|
|
BspHI |
BspEI
BsaWI |
|
XbaI |
BclI |
|
EaeI |
BsrGI
TatI |
|
|
|
|
| T A |
|
|
|
|
|
|
|
|
|
|
|
|
|
BstBI |
|
|
| T A |
|
SnaBI
BsaAI |
|
|
|
|
NruI |
|
|
FspI |
MscI |
|
PsiI |
|
|
DraI |
| T A |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| TA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Palindromic Penta-Nucleotide Recognition Sequences PDF
of this table top
| |
AA TT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
 |
N |
 |
|
|
|
|
|
|
BssKI
StyD4I |
|
|
|
|
|
MaeIII |
|
|
|
|
 |
N |
|
|
|
|
|
|
|
|
|
DdeI |
HinfI |
|
Sau96I |
|
|
|
|
|
 |
N |
|
|
|
|
|
|
|
ScrFI |
|
|
|
Fnu4HI |
|
|
|
|
|
|
|
N |
|
|
|
HpyCH4II |
|
|
|
|
|
|
|
|
|
|
|
Hpy188I |
|
|
|
N |
|
|
|
|
|
|
|
|
|
|
|
|
FmuI |
|
|
|
|
|
|
N |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
| |
A |
|
| T |
|
|
|
|
|
|
PspGI |
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
|
|
TfiI |
ApeKI
TseI |
AvaII |
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
BstNI |
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
A |
|
| T |
|
|
|
|
|
|
|
Hpy99I |
|
|
|
|
|
|
|
|
|
| |
AATT |
ACGT |
AGCT |
ATAT |
CATG |
CCGG |
CGCG |
CTAG |
GATC |
GCGC |
GGCC |
GTAC |
TATA |
TCGA |
TGCA |
TTAA |
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
Tsp45I |
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
NciI |
|
|
|
|
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
AspCNI |
|
|
|
|
|
|
|
G |
|
| C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
