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REBASE
Home >  Technical Reference > Restriction Endonucleases > Recognition Specificities
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Alphabetized List of Recognition Specificities

All restriction endonuclease recognition specificities available from New England Biolabs are listed below. For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. For example, CCTC(7/6) and (6/7)GAGG both represent an MnlI site.

All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/".

Numbers in parentheses indicate point of cleaveage for non-palindromic enzymes.
For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´

Recognition SequenceEnzyme
AA/CGTTAclI  
A/AGCTTHindIII  HindIII-HF™  
AAT/ATTSspI  SspI-HF™  
/AATTMluCI  Tsp509I  
A/CATGTPciI  
A/CCGGTAgeI  AgeI-HF™  AgeI-HF™ RE-Mix™  
ACCTGC(4/8)BfuAI  BspMI  
A/CCWGGTSexAI  
A/CGCGTMluI  
ACGGC(12/14)BceAI  
A/CGTHpyCH4IV  
ACN/GTHpyCH4III  
(10/15)ACNNNNGTAYC(12/7)BaeI  
(9/12)ACNNNNNCTCC(10/7)BsaXI  
A/CRYGTAflIII  
A/CTAGTSpeI  SpeI RE-Mix™  SpeI-HF™  
ACTGG(1/-1)BsrI  
ACTGGG(5/4)BmrI  
A/GATCTBglII  
AGC/GCTAfeI  
AG/CTAluI  
AGG/CCTStuI  
AGT/ACTScaI  ScaI-HF™ RE-Mix™  ScaI-HF™  
AT/CGATBspDI  ClaI  
ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19)PI-SceI  
ATGCA/TNsiI  
AT/TAATAseI  
ATTT/AAATSwaI  
(11/13)CAANNNNNGTGG(12/10)CspCI  
C/AATTGMfeI  MfeI-HF™ RE-Mix™  MfeI-HF™  
CACGAG(-5/-1)BssSI  
CACGTC(-3/-3)BmgBI  
CAC/GTGPmlI  
CACNNN/GTGDraIII  DraIII-HF™  
CACNN/NNGTGAleI  
CAGCAG(25/27)EcoP15I  
CAG/CTGPvuII  PvuII-HF™  
CAGNNN/CTGAlwNI  
CAGTG(2/0)BtsIMutI  
NNCASTGNN/TspRI  
CA/TATGNdeI  
C/ATGCviAII  
/CATGFatI  
CATG/NlaIII  
CAYNN/NNRTGMslI  
CC(12/16)FspEI  
CCANNNNN/NNNNTGGXcmI  
CCANNNNN/NTGGBstXI  
CCANNNN/NTGGPflMI  
CCATC(4/5)BccI  
C/CATGGNcoI  NcoI-HF™ RE-Mix™  NcoI-HF™  
CCCAGC(-5/-1)BseYI  
CCCGC(4/6)FauI  
CCC/GGGSmaI  
C/CCGGGTspMI  XmaI  
(0/-1)CCDNt.CviPII  
CCDG(10/14)LpnPI  
CCGC(-3/-1)AciI  
CCGC/GGSacII  
CCGCTC(-3/-3)BsrBI  
C/CGGHpaII  MspI  
/CCNGGBssKI  
CC/NGGScrFI  
/CCNGGStyD4I  
C/CNNGGBsaJI  
CCNNNNN/NNGGBslI  
C/CRYGGBtgI  
CC/SGGNciI  
C/CTAGGAvrII  
CCTC(7/6)MnlI  
CCTCAGC(-5/-2)BbvCI  
CCTCAGCNb.BbvCI  
CCTCAGC(-5/-7)Nt.BbvCI  
CCTGCA/GGSbfI  SbfI-HF™  
CCTNAGC(-5/-2)Bpu10I  
CC/TNAGGBsu36I  
CCTNN/NNNAGGEcoNI  
CCTTC(6/5)HpyAV  
CC/WGGBstNI  
/CCWGGPspGI  
C/CWWGGStyI  StyI-HF™  
(10/12)CGANNNNNNTGC(12/10)BcgI  
CGAT/CGPvuI  PvuI-HF™  
CG/CGBstUI  
C/GGCCGEagI  EagI-HF™  
CG/GWCCGRsrII  
CGRY/CGBsiEI  
CGTAACTATAACGGTCCTAAGGTAGCGAA(-9/-13)I-CeuI  
C/GTACGBsiWI  
CGTCTC(1/5)BsmBI  
CGWCG/Hpy99I  
CMG/CKGMspA1I  
CNNR(9/13)MspJI  
CR/CCGGYGSgrAI  
C/TAGBfaI  
CTCAG(9/7)BspCNI  
C/TCGAGPaeR7I  TliI  XhoI  XhoI RE-Mix™  
CTCTTC(1/4)EarI  
CTGAAG(16/14)AcuI  
CTGCA/GPstI  PstI-HF™  
CTGGAG(16/14)BpmI  
C/TNAGDdeI  
C/TRYAGSfcI  
C/TTAAGAflII  
CTTGAG(16/14)BpuEI  
C/TYRAGSmlI  
C/YCGRGAvaI  BsoBI  
GAAGA(8/7)MboII  
GAAGAC(2/6)BbsI  
GAANN/NNTTCXmnI  
GAATGC(1/-1)BsmI  
GAATGCNb.BsmI  
G/AATTCEcoRI  EcoRI-HF™ RE-Mix™  EcoRI-HF™  
GACGC(5/10)HgaI  
GACGT/CAatII  
GAC/GTCZraI  
GACN/NNGTCPflFI  Tth111I  
GACNN/NNGTCPshAI  
GACNNN/NNGTCAhdI  
GACNNNN/NNGTCDrdI  
GAG/CTCEco53kI  
GAGCT/CSacI  SacI-HF™  
GAGGAG(10/8)BseRI  
GAGTC(5/5)MlyI  
GAGTC(4/-5)Nt.BstNBI  
GAGTC(4/5)PleI  
G/ANTCHinfI  
GAT/ATCEcoRV  EcoRV-HF™ RE-Mix™  EcoRV-HF™  
/GATCBfuCI  
GA/TCDpnI  
/GATCDpnII  MboI  Sau3AI  
GATNN/NNATCBsaBI  
G/AWTCTfiI  
GCAATG(2/0)BsrDI  
GCAATGNb.BsrDI  
GCAGC(8/12)BbvI  
GCAGTG(2/0)BtsI  
GCAGTGNb.BtsI  
GCANNNN/NTGCBstAPI  
GCATC(5/9)SfaNI  
GCATG/CSphI  SphI-HF™  
GCCGAG(21/19)NmeAIII  
GCC/GGCNaeI  
G/CCGGCNgoMIV  
GCCNNNN/NGGCBglI  
GCGAT/CGCAsiSI  
GCGATG(10/14)BtgZI  
GCG/CHhaI  
G/CGCHinP1I  
G/CGCGCBssHII  
GC/GGCCGCNotI  NotI-HF™ RE-Mix™  NotI-HF™  
GC/NGCFnu4HI  
GCN/NGCCac8I  
GCNNNNN/NNGCMwoI  
GCTAG/CBmtI  BmtI-HF™  
G/CTAGCNheI  NheI-HF™ RE-Mix™  NheI-HF™  
GCTCTTC(1/4)BspQI  
GCTCTTC(1/-7)Nt.BspQI  
GCTCTTC(1/4)SapI  
GC/TNAGCBlpI  
G/CWGCApeKI  TseI  
GDGCH/CBsp1286I  
GGATC(4/5)AlwI  
GGATC(4/-5)Nt.AlwI  
G/GATCCBamHI  BamHI-HF™  
GGATG(2/0)BtsCI  
GGATG(9/13)FokI  
GG/CCHaeIII  PhoI  
GGCCGG/CCFseI  
GGCCNNNN/NGGCCSfiI  
G/GCGCCKasI  
GG/CGCCNarI  
GGC/GCCSfoI  
GG/CGCGCCAscI  AscI RE-Mix™  
GGCGGA(11/9)EciI  
GGGAC(10/14)BsmFI  
GGGCC/CApaI  
G/GGCCCPspOMI  
G/GNCCSau96I  
GGN/NCCNlaIV  
G/GTACCAcc65I  
GGTAC/CKpnI  KpnI-HF™  KpnI-HF™ RE-Mix™  
GGTCTC(1/5)BsaI  BsaI-HF™  
GGTGA(8/7)HphI  
G/GTNACCBstEII  BstEII-HF™  BstEII-HF™ RE-Mix™  
G/GWCCAvaII  
G/GYRCCBanI  
GKGCM/CBaeGI  
GR/CGYCBsaHI  
GRGCY/CBanII  
G/TACCviQI  
GT/ACRsaI  
GTA/TACBstZ17I  
GTATCC(6/5)BciVI  
G/TCGACSalI  SalI-HF™ RE-Mix™  SalI-HF™  
GTCTC(1/5)BcoDI  BsmAI  
GTCTC(1/-5)Nt.BsmAI  
G/TGCACApaLI  
GTGCAG(16/14)BsgI  
GT/MKACAccI  
GTN/NACHpy166II  
/GTSACTsp45I  
GTT/AACHpaI  
GTTT/AAACPmeI  
GTY/RACHincII  
GWGCW/CBsiHKAI  
R/AATTYApoI  
RCATG/YNspI  
R/CCGGYBsrFI  
R/GATCYBstYI  
RGCGC/YHaeII  
RG/CYCviKI-1  
RG/GNCCYEcoO109I  
RG/GWCCYPpuMI  
TAC/GTASnaBI  
TAGGGATAACAGGGTAAT(-9/-13)I-SceI  
T/CATGABspHI  
T/CCGGABspEI  
TCCRAC(20/18)MmeI  
T/CGATaqαI  
TCG/CGANruI  
TCN/GAHpy188I  
TC/NNGAHpy188III  
T/CTAGAXbaI  XbaI RE-Mix™  
T/GATCABclI  
TG/CAHpyCH4V  
TGC/GCAFspI  
TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17)PI-PspI  
TGG/CCAMscI  
T/GTACABsrGI  
T/TAAMseI  
TTAAT/TAAPacI  PacI RE-Mix™  
TTA/TAAPsiI  
TT/CGAABstBI  
TTT/AAADraI  
VC/TCGAGBPspXI  
W/CCGGWBsaWI  
YAC/GTRBsaAI  
Y/GGCCREaeI