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Home >  Technical Reference > Restriction Endonucleases
Alphabetized List of Recognition Specificities

All restriction endonuclease recognition specificities available from New England Biolabs are listed below. All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/". Numbers in parentheses indicate point of cleaveage for non-palindromic enzymes.

For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´

Recognition SequenceEnzyme
AA/CGTTAclI
A/AGCTTHindIII
AAT/ATTSspI
/AATTTsp509I
A/CATGTPciI
A/CCGGTAgeI
ACCTGC(4/8)BfuAI
ACCTGC(4/8)BspMI
A/CCWGGTSexAI
A/CGCGTMluI
ACGGC(12/14)BceAI
A/CGTHpyCH4IV
ACN/GTHpyCH4III
(10/15)ACNNNNGTAYC(12/7)BaeI
(9/12)ACNNNNNCTCC(10/7)BsaXI
A/CRYGTAflIII
A/CTAGTSpeI
ACTGG(1/-1)BsrI
ACTGGG(5/4)BmrI
A/GATCTBglII
AGC/GCTAfeI
AG/CTAluI
AGG/CCTStuI
AGT/ACTScaI
AGT/ACTScaI-HF™
AT/CGATBspDI
AT/CGATClaI
ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19)PI-SceI
ATGCA/TNsiI
AT/TAATAseI
ATTT/AAATSwaI
(11/13)CAANNNNNGTGG(12/10)CspCI
C/AATTGMfeI
CACGAG(-5/-1)BssSI
CACGTC(-3/-3)BmgBI
CAC/GTGPmlI
CACNNN/GTGDraIII
CACNN/NNGTGAleI
CAGCAG(25/27)EcoP15I
CAG/CTGPvuII
CAG/CTGPvuII-HF™
CAGNNN/CTGAlwNI
NNCASTGNN/TspRI
CA/TATGNdeI
CATG/NlaIII
/CATGFatI
C/ATGCviAII
CAYNN/NNRTGMslI
CCANNNNN/NNNNTGGXcmI
CCANNNNN/NTGGBstXI
CCANNNNN/NTGGBstXI
CCANNNN/NTGGPflMI
CCATC(4/5)BccI
C/CATGGNcoI
CCCAGC(-5/-1)BseYI
CCCGC(4/6)FauI
C/CCGGGXmaI
C/CCGGGTspMI
CCC/GGGSmaI
(0/-1)CCDNt.CviPII
CCGC(-3/-1)AciI
CCGC/GGSacII
CCGCTC(-3/-3)BsrBI
C/CGGHpaII
C/CGGMspI
/CCNGGStyD4I
CC/NGGScrFI
/CCNGGBssKI
C/CNNGGBsaJI
CCNNNNN/NNGGBslI
C/CRYGGBtgI
CC/SGGNciI
C/CTAGGAvrII
CCTC(7/6)MnlI
CCTCAGCNb.BbvCI
CCTCAGC(-5/-7)Nt.BbvCI
CCTCAGC(-5/-2)BbvCI
CCTGCA/GGSbfI
CCTNAGC(-5/-2)Bpu10I
CC/TNAGGBsu36I
CCTNN/NNNAGGEcoNI
CCTTC(6/5)HpyAV
CC/WGGBstNI
/CCWGGPspGI
C/CWWGGStyI
(10/12)CGANNNNNNTGC(12/10)BcgI
CGAT/CGPvuI
CG/CGBstUI
C/GGCCGEagI
CG/GWCCGRsrII
CGRY/CGBsiEI
CGTAACTATAACGGTCCTAAGGTAGCGAA(-9/-13)I-CeuI
C/GTACGBsiWI
CGTCTC(1/5)BsmBI
CGWCG/Hpy99I
CMG/CKGMspA1I
CR/CCGGYGSgrAI
C/TAGBfaI
CTCAG(9/7)BspCNI
C/TCGAGPaeR7I
C/TCGAGXhoI
C/TCGAGTliI
CTCTTC(1/4)EarI
CTGAAG(16/14)AcuI
CTGCA/GPstI
CTGGAG(16/14)BpmI
C/TNAGDdeI
C/TRYAGSfcI
C/TTAAGAflII
CTTGAG(16/14)BpuEI
C/TYRAGSmlI
C/YCGRGAvaI
C/YCGRGBsoBI
GAAGA(8/7)MboII
GAAGAC(2/6)BbsI
GAANN/NNTTCXmnI
GAATGCNb.BsmI
GAATGC(1/-1)BsmI
G/AATTCEcoRI
GACGC(5/10)HgaI
GACGT/CAatII
GAC/GTCZraI
GACN/NNGTCTth111I
GACN/NNGTCPflFI
GACNN/NNGTCPshAI
GACNNN/NNGTCAhdI
GACNNNN/NNGTCDrdI
GAGCT/CSacI
GAGGAG(10/8)BseRI
GAGTC(4/5)PleI
GAGTC(4/-5)Nt.BstNBI
GAGTC(5/5)MlyI
G/ANTCHinfI
GAT/ATCEcoRV
GA/TCDpnI
/GATCDpnII
/GATCBfuCI
/GATCMboI
/GATCSau3AI
GATNN/NNATCBsaBI
G/AWTCTfiI
GCAATGNb.BsrDI
GCAATG(2/0)BsrDI
GCAGC(8/12)BbvI
GCAGTG(2/0)BtsI
GCAGTGNb.BtsI
GCANNNN/NTGCBstAPI
GCATC(5/9)SfaNI
GCATG/CSphI
GCATG/CSphI-HF™
GCCGAG(21/19)NmeAIII
GCC/GGCNaeI
G/CCGGCNgoMIV
GCCNNNN/NGGCBglI
GCGAT/CGCAsiSI
GCGATG(10/14)BtgZI
G/CGCHinP1I
GCG/CHhaI
G/CGCGCBssHII
GC/GGCCGCNotI
GC/NGCFnu4HI
GCN/NGCCac8I
GCNNNNN/NNGCMwoI
G/CTAGCNheI
G/CTAGCNheI-HF™
GCTAG/CBmtI
GCTCTTC(1/4)BspQI
GCTCTTC(1/-7)Nt.BspQI
GCTCTTC(1/4)SapI
GC/TNAGCBlpI
G/CWGCApeKI
G/CWGCTseI
GDGCH/CBsp1286I
GGATC(4/5)AlwI
GGATC(4/-5)Nt.AlwI
G/GATCCBamHI
GGATG(2/0)BtsCI
GGATG(9/13)FokI
GG/CCHaeIII
GG/CCPhoI
GGCCGG/CCFseI
GGCCNNNN/NGGCCSfiI
GGC/GCCSfoI
GG/CGCCNarI
G/GCGCCKasI
GG/CGCGCCAscI
GGCGGA(11/9)EciI
GGGAC(10/14)BsmFI
GGGCC/CApaI
G/GGCCCPspOMI
G/GNCCSau96I
GGN/NCCNlaIV
GGTAC/CKpnI
G/GTACCAcc65I
GGTCTC(1/5)BsaI
GGTGA(8/7)HphI
G/GTNACCBstEII
G/GWCCAvaII
G/GYRCCBanI
GKGCM/CBaeGI
GR/CGYCBsaHI
GRGCY/CBanII
G/TACCviQI
GT/ACRsaI
GTA/TACBstZ17I
GTATCC(6/5)BciVI
G/TCGACSalI
G/TCGACSalI-HF™
GTCTC(1/5)BsmAI
G/TGCACApaLI
GTGCAG(16/14)BsgI
GT/MKACAccI
GTN/NACHpy166II
/GTSACTsp45I
GTT/AACHpaI
GTTT/AAACPmeI
GTY/RACHincII
GWGCW/CBsiHKAI
R/AATTYApoI
RCATG/YNspI
R/CCGGYBsrFI
R/GATCYBstYI
RGCGC/YHaeII
RG/CYCviKI-1
RG/GNCCYEcoO109I
RG/GWCCYPpuMI
TAC/GTASnaBI
TAGGGATAACAGGGTAAT(-9/-13)I-SceI
T/CATGABspHI
T/CCGGABspEI
TCCRAC(20/18)MmeI
T/CGATaqαI
TCG/CGANruI
TCN/GAHpy188I
TC/NNGAHpy188III
T/CTAGAXbaI
T/GATCABclI
TG/CAHpyCH4V
TGC/GCAFspI
TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17)PI-PspI
TGG/CCAMscI
T/GTACABsrGI
T/TAAMseI
TTAAT/TAAPacI
TTA/TAAPsiI
TT/CGAABstBI
TTT/AAADraI
VC/TCGAGBPspXI
W/CCGGWBsaWI
YAC/GTRBsaAI
Y/GGCCREaeI